Abstract
Language | English |
---|---|
Pages | 1404-1408 |
Number of pages | 5 |
Journal | Biochimica et Biophysica Acta - General Subjects |
Volume | 1860 |
Issue number | 7 |
Early online date | 7 Apr 2016 |
DOIs | |
Publication status | Published - 1 Jul 2016 |
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Keywords
- ionization
- activation
- enzyme
- ionic liquid
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Ionization basis for activation of enzymes soluble in ionic liquids. / Ou, Guangnan; He, Biyan; Halling, Peter.
In: Biochimica et Biophysica Acta - General Subjects, Vol. 1860, No. 7, 01.07.2016, p. 1404-1408.Research output: Contribution to journal › Article
TY - JOUR
T1 - Ionization basis for activation of enzymes soluble in ionic liquids
AU - Ou, Guangnan
AU - He, Biyan
AU - Halling, Peter
PY - 2016/7/1
Y1 - 2016/7/1
N2 - BACKGROUND: The complex interactions between electrolytes and proteins have been studied for more than a century. However, understanding is not yet complete and does not provide a basis for predicting the activity of enzymes in ionic media. The use of ionic liquids (ILs) as reaction medium has opened up new opportunities for better understanding of the mechanism of enzymatic catalysis. Although a number of properties of ILs have been correlated with enzyme function, these relationships are not completely understood at a molecular level. METHODS: We propose that ILs must be able to promote ionization of protein ionizable groups in order to dissolve active enzymes. The biocompatible IL need to possess a functional group with large donor number and acceptor number in both cationic and anionic units, each of which is based on a high dielectric constant lead structure. We designed and synthesized two series of ILs and determined their ionizing-dissociating abilities and activities of lipases soluble in these new ILs. RESULTS: The results showed that the ionizing-dissociating abilities of ILs paralleled the catalytic activity trend of lipases dissolved in the ILs. The activities of lipases soluble in the newly designed ILs were comparable to those in water. CONCLUSIONS: We can conclude that ionizing-dissociating abilities of an IL can be used as a basis for predicting the activity of enzymes soluble in the IL. GENERAL SIGNIFICANCE: Ionization basis for activation of enzymes gives a deeper understanding of the behavior of enzymes in non-aqueous media at a molecular level.
AB - BACKGROUND: The complex interactions between electrolytes and proteins have been studied for more than a century. However, understanding is not yet complete and does not provide a basis for predicting the activity of enzymes in ionic media. The use of ionic liquids (ILs) as reaction medium has opened up new opportunities for better understanding of the mechanism of enzymatic catalysis. Although a number of properties of ILs have been correlated with enzyme function, these relationships are not completely understood at a molecular level. METHODS: We propose that ILs must be able to promote ionization of protein ionizable groups in order to dissolve active enzymes. The biocompatible IL need to possess a functional group with large donor number and acceptor number in both cationic and anionic units, each of which is based on a high dielectric constant lead structure. We designed and synthesized two series of ILs and determined their ionizing-dissociating abilities and activities of lipases soluble in these new ILs. RESULTS: The results showed that the ionizing-dissociating abilities of ILs paralleled the catalytic activity trend of lipases dissolved in the ILs. The activities of lipases soluble in the newly designed ILs were comparable to those in water. CONCLUSIONS: We can conclude that ionizing-dissociating abilities of an IL can be used as a basis for predicting the activity of enzymes soluble in the IL. GENERAL SIGNIFICANCE: Ionization basis for activation of enzymes gives a deeper understanding of the behavior of enzymes in non-aqueous media at a molecular level.
KW - ionization
KW - activation
KW - enzyme
KW - ionic liquid
UR - http://www.sciencedirect.com/science/journal/03044165
U2 - 10.1016/j.bbagen.2016.04.004
DO - 10.1016/j.bbagen.2016.04.004
M3 - Article
VL - 1860
SP - 1404
EP - 1408
JO - BBA - General Subjects
T2 - BBA - General Subjects
JF - BBA - General Subjects
SN - 0304-4165
IS - 7
ER -