Investigation into omacetaxine solution stability for in vitro study

Lamin Marenah, Elaine Allen, Joanne Mountford, Tessa Holyoake, Heather Jorgensen, Moira Elliott

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1 Citation (Scopus)

Abstract

Omacetaxine is a natural product extract originating from Chinese medicine and finding therapeutic use as a potent myelosuppressive agent in leukemia. When planning in vitro cell biology experiments to assess omacetaxine activity against primary leukemic stem cells, it became apparent that the literature rarely describes the in vitro stability of the molecule, although accessible chromatographic methods have been published. Clearly whole organisms vs their component cells will differ in the way in which they handle xenobiotics, with the latter more dependent on physiochemical parameters such as pH and temperature in the absence of active metabolism or excretion. This could impact on the cells’ experience of drug in culture. We therefore report here on examination of a modified, high-performance liquid chromatography (HPLC) method with assessment of degradant production from a 72 h solution stability study, clearly demonstrating that omacetaxine is highly stable in representative cell culture conditions (37 °C, neutral pH) and persists for many days in marked contrast to its short-half life in vivo.
Original languageEnglish
Pages (from-to)545-547
Number of pages3
JournalBiomedical Chromatography
Volume26
Issue number5
Early online date8 Aug 2011
DOIs
Publication statusPublished - May 2012

Keywords

  • omacetaxine solution stability
  • myelosuppressive agent
  • leukemia

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  • Cite this

    Marenah, L., Allen, E., Mountford, J., Holyoake, T., Jorgensen, H., & Elliott, M. (2012). Investigation into omacetaxine solution stability for in vitro study. Biomedical Chromatography, 26(5), 545-547. https://doi.org/10.1002/bmc.1686