Investigating the variability of airborne bacteria in a hospital Intensive Care Unit over a 24 hour period

L. Dougall, M.G. Booth, E. Khoo, H. Hood, S.J. MacGregor, J.G. Anderson, M. MacLean

Research output: Contribution to conferencePoster

Abstract

Current knowledge of the clinical microflora is limited, however it is currently estimated that 10-33% of nosocomial infections are transmitted via air. This study aims to assess the variability and dynamics of airborne contamination in a hospital setting, and contribute to an improved understanding of the airborne bioburden in clinical environments.
Environmental air monitoring, using a sieve-impactor air sampler, was conducted in an Intensive Care Unit, with 500L air samples collected every 15-min over 24-h periods (08:00-08:00h). Room activity was logged and bacterial contamination levels were recorded as CFU/m3 of air. A cascade impactor and aerosol spectrometer were also used to separate particles into size fractions correlating to human lung deposition.
A high degree of variability in airborne contamination was observed over the course of a 24-h period in the hospital ICU. Contamination counts ranged from 12-510 CFU/m3 over 24-h in an isolation room occupied for 10 days by a patient with C. difficile infection. Levels were lowest during the night and in unoccupied rooms, with an average value of 20 CFU/m3. Peaks in airborne contamination showed a direct relation to an increase in room activity, such as bed changes and visiting times.

This study demonstrates the variation and degree of airborne contamination that can occur in an ICU over a 24-h period. Numerous factors contributed to increasing microbial air contamination and consideration should be given to potential improved infection control strategies and decontamination technologies to reduce airborne contamination, with the ultimate aim of reducing healthcare-associated infections from environmental sources.

Conference

ConferenceHealthcare Infection Society (HIS) Conference 2018
Abbreviated titleHIS 2018
CountryUnited Kingdom
CityLiverpool
Period26/11/1828/11/18
Internet address

Fingerprint

Intensive Care Units
Air
Bacteria
Cross Infection
Decontamination
Environmental Monitoring
Infection Control
Aerosols
Particle Size
Technology
Lung
Infection

Keywords

  • airborne bacteria
  • intensive care unit
  • microorganisms

Cite this

Dougall, L., Booth, M. G., Khoo, E., Hood, H., MacGregor, S. J., Anderson, J. G., & MacLean, M. (2018). Investigating the variability of airborne bacteria in a hospital Intensive Care Unit over a 24 hour period. Poster session presented at Healthcare Infection Society (HIS) Conference 2018, Liverpool, United Kingdom.
Dougall, L. ; Booth, M.G. ; Khoo, E. ; Hood, H. ; MacGregor, S.J. ; Anderson, J.G. ; MacLean, M. / Investigating the variability of airborne bacteria in a hospital Intensive Care Unit over a 24 hour period. Poster session presented at Healthcare Infection Society (HIS) Conference 2018, Liverpool, United Kingdom.1 p.
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abstract = "Current knowledge of the clinical microflora is limited, however it is currently estimated that 10-33{\%} of nosocomial infections are transmitted via air. This study aims to assess the variability and dynamics of airborne contamination in a hospital setting, and contribute to an improved understanding of the airborne bioburden in clinical environments.Environmental air monitoring, using a sieve-impactor air sampler, was conducted in an Intensive Care Unit, with 500L air samples collected every 15-min over 24-h periods (08:00-08:00h). Room activity was logged and bacterial contamination levels were recorded as CFU/m3 of air. A cascade impactor and aerosol spectrometer were also used to separate particles into size fractions correlating to human lung deposition. A high degree of variability in airborne contamination was observed over the course of a 24-h period in the hospital ICU. Contamination counts ranged from 12-510 CFU/m3 over 24-h in an isolation room occupied for 10 days by a patient with C. difficile infection. Levels were lowest during the night and in unoccupied rooms, with an average value of 20 CFU/m3. Peaks in airborne contamination showed a direct relation to an increase in room activity, such as bed changes and visiting times. This study demonstrates the variation and degree of airborne contamination that can occur in an ICU over a 24-h period. Numerous factors contributed to increasing microbial air contamination and consideration should be given to potential improved infection control strategies and decontamination technologies to reduce airborne contamination, with the ultimate aim of reducing healthcare-associated infections from environmental sources.",
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Dougall, L, Booth, MG, Khoo, E, Hood, H, MacGregor, SJ, Anderson, JG & MacLean, M 2018, 'Investigating the variability of airborne bacteria in a hospital Intensive Care Unit over a 24 hour period' Healthcare Infection Society (HIS) Conference 2018, Liverpool, United Kingdom, 26/11/18 - 28/11/18, .

Investigating the variability of airborne bacteria in a hospital Intensive Care Unit over a 24 hour period. / Dougall, L.; Booth, M.G.; Khoo, E.; Hood, H.; MacGregor, S.J.; Anderson, J.G.; MacLean, M.

2018. Poster session presented at Healthcare Infection Society (HIS) Conference 2018, Liverpool, United Kingdom.

Research output: Contribution to conferencePoster

TY - CONF

T1 - Investigating the variability of airborne bacteria in a hospital Intensive Care Unit over a 24 hour period

AU - Dougall, L.

AU - Booth, M.G.

AU - Khoo, E.

AU - Hood, H.

AU - MacGregor, S.J.

AU - Anderson, J.G.

AU - MacLean, M.

PY - 2018/11/26

Y1 - 2018/11/26

N2 - Current knowledge of the clinical microflora is limited, however it is currently estimated that 10-33% of nosocomial infections are transmitted via air. This study aims to assess the variability and dynamics of airborne contamination in a hospital setting, and contribute to an improved understanding of the airborne bioburden in clinical environments.Environmental air monitoring, using a sieve-impactor air sampler, was conducted in an Intensive Care Unit, with 500L air samples collected every 15-min over 24-h periods (08:00-08:00h). Room activity was logged and bacterial contamination levels were recorded as CFU/m3 of air. A cascade impactor and aerosol spectrometer were also used to separate particles into size fractions correlating to human lung deposition. A high degree of variability in airborne contamination was observed over the course of a 24-h period in the hospital ICU. Contamination counts ranged from 12-510 CFU/m3 over 24-h in an isolation room occupied for 10 days by a patient with C. difficile infection. Levels were lowest during the night and in unoccupied rooms, with an average value of 20 CFU/m3. Peaks in airborne contamination showed a direct relation to an increase in room activity, such as bed changes and visiting times. This study demonstrates the variation and degree of airborne contamination that can occur in an ICU over a 24-h period. Numerous factors contributed to increasing microbial air contamination and consideration should be given to potential improved infection control strategies and decontamination technologies to reduce airborne contamination, with the ultimate aim of reducing healthcare-associated infections from environmental sources.

AB - Current knowledge of the clinical microflora is limited, however it is currently estimated that 10-33% of nosocomial infections are transmitted via air. This study aims to assess the variability and dynamics of airborne contamination in a hospital setting, and contribute to an improved understanding of the airborne bioburden in clinical environments.Environmental air monitoring, using a sieve-impactor air sampler, was conducted in an Intensive Care Unit, with 500L air samples collected every 15-min over 24-h periods (08:00-08:00h). Room activity was logged and bacterial contamination levels were recorded as CFU/m3 of air. A cascade impactor and aerosol spectrometer were also used to separate particles into size fractions correlating to human lung deposition. A high degree of variability in airborne contamination was observed over the course of a 24-h period in the hospital ICU. Contamination counts ranged from 12-510 CFU/m3 over 24-h in an isolation room occupied for 10 days by a patient with C. difficile infection. Levels were lowest during the night and in unoccupied rooms, with an average value of 20 CFU/m3. Peaks in airborne contamination showed a direct relation to an increase in room activity, such as bed changes and visiting times. This study demonstrates the variation and degree of airborne contamination that can occur in an ICU over a 24-h period. Numerous factors contributed to increasing microbial air contamination and consideration should be given to potential improved infection control strategies and decontamination technologies to reduce airborne contamination, with the ultimate aim of reducing healthcare-associated infections from environmental sources.

KW - airborne bacteria

KW - intensive care unit

KW - microorganisms

M3 - Poster

ER -

Dougall L, Booth MG, Khoo E, Hood H, MacGregor SJ, Anderson JG et al. Investigating the variability of airborne bacteria in a hospital Intensive Care Unit over a 24 hour period. 2018. Poster session presented at Healthcare Infection Society (HIS) Conference 2018, Liverpool, United Kingdom.