Investigating the interaction forces between T cells and antigen-presenting cells using an optical trapping system

A. J. Wright, R. A. Benson, R. W. Bowman, G. M. Gibson, M. J. Padgett, J. M. Girkin, J. Brewer, P. Garside

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

2 Citations (Scopus)

Abstract

The interactions between T cells and antigen-presenting cells (APCs) are crucial in triggering a successful antigen-specific, adaptive immune response leading to protection against a particular pathogen or disease. At present very little is known about the magnitudes of the forces involved in these interactions. We present results showing for the first time that optical tweezers can be used to measure these cell-cell interaction forces. We were able to see a significant difference in the force distributions taken with and without antigen, as evidenced by a Mann-Whitney U-test. The T cells of interest were trapped directly and no exogenous beads were added to the sample. Interaction forces between T cells and APCs in the presence of specific antigen ranged from 0-6.5 pN, whereas, when the specific antigen was absent the interaction forces ranged from 0-1.5 pN. The accuracy of the system will be discussed in terms of how we tracked the position of the optically trapped cell and the methods we used to minimise cell roll.
LanguageEnglish
Title of host publicationOptical trapping and optical micromanipulation VIII
EditorsK. Dholakia, G.C. Spalding
Place of PublicationBellingham
DOIs
Publication statusPublished - 2011

Publication series

NameProceedings of SPIE
PublisherSPIE
Volume8097
ISSN (Print)0277-786X

Fingerprint

Optical Tweezers
Optical Devices
Viral Tumor Antigens
Antigen-Presenting Cells
T-Lymphocytes
Antigens
Adaptive Immunity
Nonparametric Statistics
Cell Communication

Keywords

  • tolerance
  • cellular interactions
  • mouse bone-marrow
  • t cells
  • dendritic cells
  • optical trapping
  • beam
  • tweezers
  • force measurements
  • particles
  • angular-momentum
  • microscopy
  • immune cells
  • interaction forces
  • antigen-presenting cells

Cite this

Wright, A. J., Benson, R. A., Bowman, R. W., Gibson, G. M., Padgett, M. J., Girkin, J. M., ... Garside, P. (2011). Investigating the interaction forces between T cells and antigen-presenting cells using an optical trapping system. In K. Dholakia, & G. C. Spalding (Eds.), Optical trapping and optical micromanipulation VIII (Proceedings of SPIE ; Vol. 8097). Bellingham. https://doi.org/10.1117/12.895113
Wright, A. J. ; Benson, R. A. ; Bowman, R. W. ; Gibson, G. M. ; Padgett, M. J. ; Girkin, J. M. ; Brewer, J. ; Garside, P. / Investigating the interaction forces between T cells and antigen-presenting cells using an optical trapping system. Optical trapping and optical micromanipulation VIII. editor / K. Dholakia ; G.C. Spalding. Bellingham, 2011. (Proceedings of SPIE ).
@inproceedings{f7abee2252c249c591577367d5c878fb,
title = "Investigating the interaction forces between T cells and antigen-presenting cells using an optical trapping system",
abstract = "The interactions between T cells and antigen-presenting cells (APCs) are crucial in triggering a successful antigen-specific, adaptive immune response leading to protection against a particular pathogen or disease. At present very little is known about the magnitudes of the forces involved in these interactions. We present results showing for the first time that optical tweezers can be used to measure these cell-cell interaction forces. We were able to see a significant difference in the force distributions taken with and without antigen, as evidenced by a Mann-Whitney U-test. The T cells of interest were trapped directly and no exogenous beads were added to the sample. Interaction forces between T cells and APCs in the presence of specific antigen ranged from 0-6.5 pN, whereas, when the specific antigen was absent the interaction forces ranged from 0-1.5 pN. The accuracy of the system will be discussed in terms of how we tracked the position of the optically trapped cell and the methods we used to minimise cell roll.",
keywords = "tolerance, cellular interactions, mouse bone-marrow, t cells, dendritic cells, optical trapping, beam, tweezers, force measurements, particles, angular-momentum, microscopy, immune cells, interaction forces , antigen-presenting cells",
author = "Wright, {A. J.} and Benson, {R. A.} and Bowman, {R. W.} and Gibson, {G. M.} and Padgett, {M. J.} and Girkin, {J. M.} and J. Brewer and P. Garside",
year = "2011",
doi = "10.1117/12.895113",
language = "English",
isbn = "9780819487070",
series = "Proceedings of SPIE",
publisher = "SPIE",
editor = "Dholakia, {K. } and Spalding, {G.C. }",
booktitle = "Optical trapping and optical micromanipulation VIII",

}

Wright, AJ, Benson, RA, Bowman, RW, Gibson, GM, Padgett, MJ, Girkin, JM, Brewer, J & Garside, P 2011, Investigating the interaction forces between T cells and antigen-presenting cells using an optical trapping system. in K Dholakia & GC Spalding (eds), Optical trapping and optical micromanipulation VIII. Proceedings of SPIE , vol. 8097, Bellingham. https://doi.org/10.1117/12.895113

Investigating the interaction forces between T cells and antigen-presenting cells using an optical trapping system. / Wright, A. J.; Benson, R. A.; Bowman, R. W.; Gibson, G. M.; Padgett, M. J.; Girkin, J. M.; Brewer, J.; Garside, P.

Optical trapping and optical micromanipulation VIII. ed. / K. Dholakia; G.C. Spalding. Bellingham, 2011. (Proceedings of SPIE ; Vol. 8097).

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

TY - GEN

T1 - Investigating the interaction forces between T cells and antigen-presenting cells using an optical trapping system

AU - Wright, A. J.

AU - Benson, R. A.

AU - Bowman, R. W.

AU - Gibson, G. M.

AU - Padgett, M. J.

AU - Girkin, J. M.

AU - Brewer, J.

AU - Garside, P.

PY - 2011

Y1 - 2011

N2 - The interactions between T cells and antigen-presenting cells (APCs) are crucial in triggering a successful antigen-specific, adaptive immune response leading to protection against a particular pathogen or disease. At present very little is known about the magnitudes of the forces involved in these interactions. We present results showing for the first time that optical tweezers can be used to measure these cell-cell interaction forces. We were able to see a significant difference in the force distributions taken with and without antigen, as evidenced by a Mann-Whitney U-test. The T cells of interest were trapped directly and no exogenous beads were added to the sample. Interaction forces between T cells and APCs in the presence of specific antigen ranged from 0-6.5 pN, whereas, when the specific antigen was absent the interaction forces ranged from 0-1.5 pN. The accuracy of the system will be discussed in terms of how we tracked the position of the optically trapped cell and the methods we used to minimise cell roll.

AB - The interactions between T cells and antigen-presenting cells (APCs) are crucial in triggering a successful antigen-specific, adaptive immune response leading to protection against a particular pathogen or disease. At present very little is known about the magnitudes of the forces involved in these interactions. We present results showing for the first time that optical tweezers can be used to measure these cell-cell interaction forces. We were able to see a significant difference in the force distributions taken with and without antigen, as evidenced by a Mann-Whitney U-test. The T cells of interest were trapped directly and no exogenous beads were added to the sample. Interaction forces between T cells and APCs in the presence of specific antigen ranged from 0-6.5 pN, whereas, when the specific antigen was absent the interaction forces ranged from 0-1.5 pN. The accuracy of the system will be discussed in terms of how we tracked the position of the optically trapped cell and the methods we used to minimise cell roll.

KW - tolerance

KW - cellular interactions

KW - mouse bone-marrow

KW - t cells

KW - dendritic cells

KW - optical trapping

KW - beam

KW - tweezers

KW - force measurements

KW - particles

KW - angular-momentum

KW - microscopy

KW - immune cells

KW - interaction forces

KW - antigen-presenting cells

UR - http://www.scopus.com/inward/record.url?scp=80054056912&partnerID=8YFLogxK

U2 - 10.1117/12.895113

DO - 10.1117/12.895113

M3 - Conference contribution book

SN - 9780819487070

T3 - Proceedings of SPIE

BT - Optical trapping and optical micromanipulation VIII

A2 - Dholakia, K.

A2 - Spalding, G.C.

CY - Bellingham

ER -

Wright AJ, Benson RA, Bowman RW, Gibson GM, Padgett MJ, Girkin JM et al. Investigating the interaction forces between T cells and antigen-presenting cells using an optical trapping system. In Dholakia K, Spalding GC, editors, Optical trapping and optical micromanipulation VIII. Bellingham. 2011. (Proceedings of SPIE ). https://doi.org/10.1117/12.895113