Investigating the immunologic effects of cocr nanoparticles

B. Ogunwale, A. Schmidt-Ott, R.M.D. Meek, J.M. Brewer

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The increase in metal-on-metal hip arthroplasties has led to concern regarding the effect of raised serum and tissue metal ion levels. Our aim was to determine changes in the integrity and function of cells of the immune system after exposure to CoCr nanoparticles in specific cell culture experiments. Nanometer-sized particles of CoCr were made from a manufacturer's forged CoCr used for metal-on-metal articulations. Primary, murine dendritic cells and T and B lymphocytes then were exposed to these CoCr particles under cell culture conditions and then assayed for viability and proliferation/activation. CoCr nanoparticles did not directly activate dendritic cells or regulate B cells. Although nanoparticles were not directly toxic to resting T cells, Signals 1- and 2-dependent T cell proliferation were reduced. This may explain the observed reduction in CD8+ T cells observed in patients with metal-on-metal implants.
Original languageEnglish
Pages (from-to)3010-3016
Number of pages6
JournalClinical Orthopaedics and Related Research
Volume467
Issue number11
DOIs
Publication statusPublished - Nov 2009

Keywords

  • total hip replacements
  • cd4 t-cells
  • spark discharge
  • mckee-farrar
  • lymph-nodes
  • metal-ions
  • in-vitro
  • prostheses
  • chromium
  • antigen

Cite this

Ogunwale, B., Schmidt-Ott, A., Meek, R. M. D., & Brewer, J. M. (2009). Investigating the immunologic effects of cocr nanoparticles. Clinical Orthopaedics and Related Research, 467(11), 3010-3016. https://doi.org/10.1007/s11999-009-0949-9