Investigating pre-analytical requirements for serum and plasma based infrared spectro-diagnostic

Lila Lovergne, Jean Lovergne, Pascaline Bouzy, Valérie Untereiner, Marc Offroy, Roselyne Garnotel, Gérard Thiéfin, Matthew J. Baker, Ganesh D. Sockalingum

Research output: Contribution to journalArticle

Abstract

Infrared spectroscopy is a rapid, easy‐to‐operate, label‐free and therefore cost‐effective technique. Many studies performed on biofluids (e.g. serum, plasma, urine, sputum, bile, cerebrospinal fluid) have demonstrated its promising application as a clinical diagnostic tool. Given all these characteristics, infrared spectroscopy appears to be an ideal candidate to be implemented into the clinics. However, before considering its translation, a clear effort is needed to standardise protocols for biofluid spectroscopic analysis. To reach this goal, careful investigations to identify and track errors that can occur during the pre‐analytical phase is a crucial step. Here, we report for the first time, results of investigations into pre‐analytical factors that can affect the quality of the spectral data acquired on serum and plasma, such as the impact of long‐term freezing time storage of samples as well as the month‐ to‐month reproducibility of the spectroscopic analysis. The spectral data discrimination has revealed to be majorly impacted by a residual water content variation in serum and plasma dried samples.
LanguageEnglish
Article numbere201900177
Number of pages19
JournalJournal of Biophotonics
Early online date5 Jul 2019
DOIs
Publication statusE-pub ahead of print - 5 Jul 2019

Fingerprint

serums
Spectroscopic analysis
spectroscopic analysis
Infrared radiation
Plasmas
requirements
Infrared spectroscopy
Spectrum Analysis
Serum
infrared spectroscopy
cerebrospinal fluid
Cerebrospinal fluid
urine
Sputum
Bile
Freezing
Water content
freezing
moisture content
Cerebrospinal Fluid

Keywords

  • FTIR spectroscopy
  • biofluids
  • serum
  • plasma
  • pre-analytical requirements
  • water interference correction

Cite this

Lovergne, L., Lovergne, J., Bouzy, P., Untereiner, V., Offroy, M., Garnotel, R., ... Sockalingum, G. D. (2019). Investigating pre-analytical requirements for serum and plasma based infrared spectro-diagnostic. Journal of Biophotonics, [e201900177]. https://doi.org/10.1002/jbio.201900177
Lovergne, Lila ; Lovergne, Jean ; Bouzy, Pascaline ; Untereiner, Valérie ; Offroy, Marc ; Garnotel, Roselyne ; Thiéfin, Gérard ; Baker, Matthew J. ; Sockalingum, Ganesh D. / Investigating pre-analytical requirements for serum and plasma based infrared spectro-diagnostic. In: Journal of Biophotonics. 2019.
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Lovergne, L, Lovergne, J, Bouzy, P, Untereiner, V, Offroy, M, Garnotel, R, Thiéfin, G, Baker, MJ & Sockalingum, GD 2019, 'Investigating pre-analytical requirements for serum and plasma based infrared spectro-diagnostic' Journal of Biophotonics. https://doi.org/10.1002/jbio.201900177

Investigating pre-analytical requirements for serum and plasma based infrared spectro-diagnostic. / Lovergne, Lila ; Lovergne, Jean ; Bouzy, Pascaline ; Untereiner, Valérie; Offroy, Marc ; Garnotel, Roselyne ; Thiéfin, Gérard; Baker, Matthew J.; Sockalingum, Ganesh D.

In: Journal of Biophotonics, 05.07.2019.

Research output: Contribution to journalArticle

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