Investigating cellular structures at the nanoscale with organic fluorophores

Sebastian van de Linde, Sarah Aufmkolk, Christian Franke, Thorge Holm, Teresa Klein, Anna Löschberger, Sven Proppert, Steve Wolter, Markus Sauer

Research output: Contribution to journalReview article

39 Citations (Scopus)

Abstract

Super-resolution fluorescence imaging can provide insights into cellular structure and organization with a spatial resolution approaching virtually electron microscopy. Among all the different super-resolution methods single-molecule-based localization microscopy could play an exceptional role in the future because it can provide quantitative information, for example, the absolute number of biomolecules interacting in space and time. Here, small organic fluorophores are a decisive factor because they exhibit high fluorescence quantum yields and photostabilities, thus enabling their localization with nanometer precision. Besides past progress, problems with high-density and specific labeling, especially in living cells, and the lack of suited standards and long-term continuous imaging methods with minimal photodamage render the exploitation of the full potential of the method currently challenging.

LanguageEnglish
Pages8-18
Number of pages11
JournalChemistry and Biology
Volume20
Issue number1
DOIs
Publication statusPublished - 24 Jan 2013

Fingerprint

Fluorophores
Cellular Structures
Fluorescence
Imaging techniques
Biomolecules
Quantum yield
Labeling
Electron microscopy
Microscopic examination
Optical Imaging
Cells
Molecules
Microscopy
Electron Microscopy

Keywords

  • animals
  • cellular Structures/ultrastructure
  • fluorescent Dyes/analysis
  • humans
  • microscopy, fluorescence/methods
  • optical Imaging/methods
  • organic fluorophores

Cite this

van de Linde, S., Aufmkolk, S., Franke, C., Holm, T., Klein, T., Löschberger, A., ... Sauer, M. (2013). Investigating cellular structures at the nanoscale with organic fluorophores. Chemistry and Biology, 20(1), 8-18. https://doi.org/10.1016/j.chembiol.2012.11.004
van de Linde, Sebastian ; Aufmkolk, Sarah ; Franke, Christian ; Holm, Thorge ; Klein, Teresa ; Löschberger, Anna ; Proppert, Sven ; Wolter, Steve ; Sauer, Markus. / Investigating cellular structures at the nanoscale with organic fluorophores. In: Chemistry and Biology. 2013 ; Vol. 20, No. 1. pp. 8-18.
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van de Linde, S, Aufmkolk, S, Franke, C, Holm, T, Klein, T, Löschberger, A, Proppert, S, Wolter, S & Sauer, M 2013, 'Investigating cellular structures at the nanoscale with organic fluorophores' Chemistry and Biology, vol. 20, no. 1, pp. 8-18. https://doi.org/10.1016/j.chembiol.2012.11.004

Investigating cellular structures at the nanoscale with organic fluorophores. / van de Linde, Sebastian; Aufmkolk, Sarah; Franke, Christian; Holm, Thorge; Klein, Teresa; Löschberger, Anna; Proppert, Sven; Wolter, Steve; Sauer, Markus.

In: Chemistry and Biology, Vol. 20, No. 1, 24.01.2013, p. 8-18.

Research output: Contribution to journalReview article

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T1 - Investigating cellular structures at the nanoscale with organic fluorophores

AU - van de Linde, Sebastian

AU - Aufmkolk, Sarah

AU - Franke, Christian

AU - Holm, Thorge

AU - Klein, Teresa

AU - Löschberger, Anna

AU - Proppert, Sven

AU - Wolter, Steve

AU - Sauer, Markus

N1 - Copyright © 2013 Elsevier Ltd. All rights reserved.

PY - 2013/1/24

Y1 - 2013/1/24

N2 - Super-resolution fluorescence imaging can provide insights into cellular structure and organization with a spatial resolution approaching virtually electron microscopy. Among all the different super-resolution methods single-molecule-based localization microscopy could play an exceptional role in the future because it can provide quantitative information, for example, the absolute number of biomolecules interacting in space and time. Here, small organic fluorophores are a decisive factor because they exhibit high fluorescence quantum yields and photostabilities, thus enabling their localization with nanometer precision. Besides past progress, problems with high-density and specific labeling, especially in living cells, and the lack of suited standards and long-term continuous imaging methods with minimal photodamage render the exploitation of the full potential of the method currently challenging.

AB - Super-resolution fluorescence imaging can provide insights into cellular structure and organization with a spatial resolution approaching virtually electron microscopy. Among all the different super-resolution methods single-molecule-based localization microscopy could play an exceptional role in the future because it can provide quantitative information, for example, the absolute number of biomolecules interacting in space and time. Here, small organic fluorophores are a decisive factor because they exhibit high fluorescence quantum yields and photostabilities, thus enabling their localization with nanometer precision. Besides past progress, problems with high-density and specific labeling, especially in living cells, and the lack of suited standards and long-term continuous imaging methods with minimal photodamage render the exploitation of the full potential of the method currently challenging.

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KW - cellular Structures/ultrastructure

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KW - microscopy, fluorescence/methods

KW - optical Imaging/methods

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