Investigating cellular structures at the nanoscale with organic fluorophores

Sebastian van de Linde, Sarah Aufmkolk, Christian Franke, Thorge Holm, Teresa Klein, Anna Löschberger, Sven Proppert, Steve Wolter, Markus Sauer

Research output: Contribution to journalReview article

41 Citations (Scopus)

Abstract

Super-resolution fluorescence imaging can provide insights into cellular structure and organization with a spatial resolution approaching virtually electron microscopy. Among all the different super-resolution methods single-molecule-based localization microscopy could play an exceptional role in the future because it can provide quantitative information, for example, the absolute number of biomolecules interacting in space and time. Here, small organic fluorophores are a decisive factor because they exhibit high fluorescence quantum yields and photostabilities, thus enabling their localization with nanometer precision. Besides past progress, problems with high-density and specific labeling, especially in living cells, and the lack of suited standards and long-term continuous imaging methods with minimal photodamage render the exploitation of the full potential of the method currently challenging.

Original languageEnglish
Pages (from-to)8-18
Number of pages11
JournalChemistry and Biology
Volume20
Issue number1
DOIs
Publication statusPublished - 24 Jan 2013

Keywords

  • animals
  • cellular Structures/ultrastructure
  • fluorescent Dyes/analysis
  • humans
  • microscopy, fluorescence/methods
  • optical Imaging/methods
  • organic fluorophores

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