Introduction to bioreactors of shake-flask inocula leads to development of oxidative stress in Aspergillus niger

Andrew O'Donnell, Brian McNeil, Zhonghu Bai, L.M. Harvey, Yantao Bai

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Inoculation of bioreactors with shake-flask cultures present the organism with an immediate shift from an environment with little O2 to one in which O2 is typically at 100% saturation. The inoculation of such shake-flasks cultures into bioreactors sparged with 1 vvm air or 1 vvm air/O2 mix i.e. 50% O2 enrichment is an oxidatively stressful event, as judged by immediate increases in the intracellular concentrations of superoxide anion radical (O2·−) (from 4,600 to 11,600 RLU mg DCW−1 and 5,500 to 23,000 RLU mg DCW−1 respectively) and changes in the activities of the major antioxidant enzymes superoxide dismutase and catalase in all cultures. There are further effects on metabolic indices, particularly decreased nutrient consumption in oxygenated cultures (from 0.16 to 0.12 g starch g DCW h−1) and decreased protein production, indicating that inoculation of the bioreactor exerts a global burden on the cellular metabolic networks.
LanguageEnglish
Pages895-900
Number of pages5
JournalBiotechnology Letters
Volume29
Issue number6
DOIs
Publication statusPublished - Mar 2007

Fingerprint

Aspergillus niger
Bioreactors
Oxidative Stress
Batch Cell Culture Techniques
Superoxides
Air
Metabolic Networks and Pathways
Starch
Catalase
Superoxide Dismutase
Antioxidants
Food
Enzymes
Proteins

Keywords

  • filamentous fungi
  • lag phase
  • oxidative stress
  • oxygen enrichment
  • pharmacology

Cite this

O'Donnell, Andrew ; McNeil, Brian ; Bai, Zhonghu ; Harvey, L.M. ; Bai, Yantao. / Introduction to bioreactors of shake-flask inocula leads to development of oxidative stress in Aspergillus niger. In: Biotechnology Letters. 2007 ; Vol. 29, No. 6. pp. 895-900.
@article{9c949e0bc16a4f4a88edc4bc14be99b6,
title = "Introduction to bioreactors of shake-flask inocula leads to development of oxidative stress in Aspergillus niger",
abstract = "Inoculation of bioreactors with shake-flask cultures present the organism with an immediate shift from an environment with little O2 to one in which O2 is typically at 100{\%} saturation. The inoculation of such shake-flasks cultures into bioreactors sparged with 1 vvm air or 1 vvm air/O2 mix i.e. 50{\%} O2 enrichment is an oxidatively stressful event, as judged by immediate increases in the intracellular concentrations of superoxide anion radical (O2·−) (from 4,600 to 11,600 RLU mg DCW−1 and 5,500 to 23,000 RLU mg DCW−1 respectively) and changes in the activities of the major antioxidant enzymes superoxide dismutase and catalase in all cultures. There are further effects on metabolic indices, particularly decreased nutrient consumption in oxygenated cultures (from 0.16 to 0.12 g starch g DCW h−1) and decreased protein production, indicating that inoculation of the bioreactor exerts a global burden on the cellular metabolic networks.",
keywords = "filamentous fungi, lag phase, oxidative stress, oxygen enrichment, pharmacology",
author = "Andrew O'Donnell and Brian McNeil and Zhonghu Bai and L.M. Harvey and Yantao Bai",
year = "2007",
month = "3",
doi = "10.1007/s10529-007-9336-3",
language = "English",
volume = "29",
pages = "895--900",
journal = "Biotechnology Letters",
issn = "0141-5492",
number = "6",

}

Introduction to bioreactors of shake-flask inocula leads to development of oxidative stress in Aspergillus niger. / O'Donnell, Andrew; McNeil, Brian; Bai, Zhonghu; Harvey, L.M.; Bai, Yantao.

In: Biotechnology Letters, Vol. 29, No. 6, 03.2007, p. 895-900.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Introduction to bioreactors of shake-flask inocula leads to development of oxidative stress in Aspergillus niger

AU - O'Donnell, Andrew

AU - McNeil, Brian

AU - Bai, Zhonghu

AU - Harvey, L.M.

AU - Bai, Yantao

PY - 2007/3

Y1 - 2007/3

N2 - Inoculation of bioreactors with shake-flask cultures present the organism with an immediate shift from an environment with little O2 to one in which O2 is typically at 100% saturation. The inoculation of such shake-flasks cultures into bioreactors sparged with 1 vvm air or 1 vvm air/O2 mix i.e. 50% O2 enrichment is an oxidatively stressful event, as judged by immediate increases in the intracellular concentrations of superoxide anion radical (O2·−) (from 4,600 to 11,600 RLU mg DCW−1 and 5,500 to 23,000 RLU mg DCW−1 respectively) and changes in the activities of the major antioxidant enzymes superoxide dismutase and catalase in all cultures. There are further effects on metabolic indices, particularly decreased nutrient consumption in oxygenated cultures (from 0.16 to 0.12 g starch g DCW h−1) and decreased protein production, indicating that inoculation of the bioreactor exerts a global burden on the cellular metabolic networks.

AB - Inoculation of bioreactors with shake-flask cultures present the organism with an immediate shift from an environment with little O2 to one in which O2 is typically at 100% saturation. The inoculation of such shake-flasks cultures into bioreactors sparged with 1 vvm air or 1 vvm air/O2 mix i.e. 50% O2 enrichment is an oxidatively stressful event, as judged by immediate increases in the intracellular concentrations of superoxide anion radical (O2·−) (from 4,600 to 11,600 RLU mg DCW−1 and 5,500 to 23,000 RLU mg DCW−1 respectively) and changes in the activities of the major antioxidant enzymes superoxide dismutase and catalase in all cultures. There are further effects on metabolic indices, particularly decreased nutrient consumption in oxygenated cultures (from 0.16 to 0.12 g starch g DCW h−1) and decreased protein production, indicating that inoculation of the bioreactor exerts a global burden on the cellular metabolic networks.

KW - filamentous fungi

KW - lag phase

KW - oxidative stress

KW - oxygen enrichment

KW - pharmacology

UR - http://dx.doi.org/10.1007/s10529-007-9336-3

U2 - 10.1007/s10529-007-9336-3

DO - 10.1007/s10529-007-9336-3

M3 - Article

VL - 29

SP - 895

EP - 900

JO - Biotechnology Letters

T2 - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

IS - 6

ER -