Introduction of permanently charged groups into PEGA resins leads to improved biotransformations on solid support

A Basso, R V Ulijn, S L Flitsch, G Margetts, I Brazendale, C Ebert, L De Martin, P Linda, S Verdelli, L Gardossi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The application of biotransformations in solid phase synthesis is an attractive alternative to chemical methodologies. An important issue that needs to be addressed in this context is accessibility of functional groups within a porous polymer to the biocatalyst. This paper shows that such accessibility can be improved for penicillin G amidase by introducing permanent charges into the polymer. The effect appears to be due to better swelling of the polymer in buffer and to electrostatic interactions between polymer and enzyme. (C) 2003 Elsevier Ltd. All rights reserved.

LanguageEnglish
Pages589-594
Number of pages6
JournalTetrahedron
Volume60
Issue number3
DOIs
Publication statusPublished - 12 Jan 2004

Fingerprint

Biotransformation
Polymers
amidase
Penicillin Amidase
Solid-Phase Synthesis Techniques
Penicillin G
Enzymes
Coulomb interactions
Static Electricity
Functional groups
Swelling
Buffers
poly(acryloyl-bis(aminopropyl)polyethylene glycol)

Keywords

  • permanently charged groups
  • PEGA resins
  • improved biotransformations
  • solid support

Cite this

Basso, A ; Ulijn, R V ; Flitsch, S L ; Margetts, G ; Brazendale, I ; Ebert, C ; De Martin, L ; Linda, P ; Verdelli, S ; Gardossi, L . / Introduction of permanently charged groups into PEGA resins leads to improved biotransformations on solid support. In: Tetrahedron. 2004 ; Vol. 60, No. 3. pp. 589-594.
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Basso, A, Ulijn, RV, Flitsch, SL, Margetts, G, Brazendale, I, Ebert, C, De Martin, L, Linda, P, Verdelli, S & Gardossi, L 2004, 'Introduction of permanently charged groups into PEGA resins leads to improved biotransformations on solid support' Tetrahedron, vol. 60, no. 3, pp. 589-594. https://doi.org/10.1016/j.tet.2003.10.125

Introduction of permanently charged groups into PEGA resins leads to improved biotransformations on solid support. / Basso, A ; Ulijn, R V ; Flitsch, S L ; Margetts, G ; Brazendale, I ; Ebert, C ; De Martin, L ; Linda, P ; Verdelli, S ; Gardossi, L .

In: Tetrahedron, Vol. 60, No. 3, 12.01.2004, p. 589-594.

Research output: Contribution to journalArticle

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T1 - Introduction of permanently charged groups into PEGA resins leads to improved biotransformations on solid support

AU - Basso, A

AU - Ulijn, R V

AU - Flitsch, S L

AU - Margetts, G

AU - Brazendale, I

AU - Ebert, C

AU - De Martin, L

AU - Linda, P

AU - Verdelli, S

AU - Gardossi, L

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AB - The application of biotransformations in solid phase synthesis is an attractive alternative to chemical methodologies. An important issue that needs to be addressed in this context is accessibility of functional groups within a porous polymer to the biocatalyst. This paper shows that such accessibility can be improved for penicillin G amidase by introducing permanent charges into the polymer. The effect appears to be due to better swelling of the polymer in buffer and to electrostatic interactions between polymer and enzyme. (C) 2003 Elsevier Ltd. All rights reserved.

KW - permanently charged groups

KW - PEGA resins

KW - improved biotransformations

KW - solid support

U2 - 10.1016/j.tet.2003.10.125

DO - 10.1016/j.tet.2003.10.125

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