Introduction of permanently charged groups into PEGA resins leads to improved biotransformations on solid support

A  Basso; R V  Ulijn; S L  Flitsch; G  Margetts; I  Brazendale; C  Ebert; L  De Martin; P  Linda; S  Verdelli; L  Gardossi

doi:10.1016/j.tet.2003.10.125

Introduction of permanently charged groups into PEGA resins leads to improved biotransformations on solid support

A Basso, R V Ulijn, S L Flitsch, G Margetts, I Brazendale, C Ebert, L De Martin, P Linda, S Verdelli, L Gardossi

Pure And Applied Chemistry

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

The application of biotransformations in solid phase synthesis is an attractive alternative to chemical methodologies. An important issue that needs to be addressed in this context is accessibility of functional groups within a porous polymer to the biocatalyst. This paper shows that such accessibility can be improved for penicillin G amidase by introducing permanent charges into the polymer. The effect appears to be due to better swelling of the polymer in buffer and to electrostatic interactions between polymer and enzyme. (C) 2003 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	589-594
Number of pages	6
Journal	Tetrahedron
Volume	60
Issue number	3
DOIs	https://doi.org/10.1016/j.tet.2003.10.125
Publication status	Published - 12 Jan 2004

Keywords

permanently charged groups
PEGA resins
improved biotransformations
solid support

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10.1016/j.tet.2003.10.125

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title = "Introduction of permanently charged groups into PEGA resins leads to improved biotransformations on solid support",

abstract = "The application of biotransformations in solid phase synthesis is an attractive alternative to chemical methodologies. An important issue that needs to be addressed in this context is accessibility of functional groups within a porous polymer to the biocatalyst. This paper shows that such accessibility can be improved for penicillin G amidase by introducing permanent charges into the polymer. The effect appears to be due to better swelling of the polymer in buffer and to electrostatic interactions between polymer and enzyme. (C) 2003 Elsevier Ltd. All rights reserved.",

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author = "A Basso and Ulijn, {R V} and Flitsch, {S L} and G Margetts and I Brazendale and C Ebert and {De Martin}, L and P Linda and S Verdelli and L Gardossi",

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T1 - Introduction of permanently charged groups into PEGA resins leads to improved biotransformations on solid support

AU - Basso, A

AU - Ulijn, R V

AU - Flitsch, S L

AU - Margetts, G

AU - Brazendale, I

AU - Ebert, C

AU - De Martin, L

AU - Linda, P

AU - Verdelli, S

AU - Gardossi, L

PY - 2004/1/12

Y1 - 2004/1/12

N2 - The application of biotransformations in solid phase synthesis is an attractive alternative to chemical methodologies. An important issue that needs to be addressed in this context is accessibility of functional groups within a porous polymer to the biocatalyst. This paper shows that such accessibility can be improved for penicillin G amidase by introducing permanent charges into the polymer. The effect appears to be due to better swelling of the polymer in buffer and to electrostatic interactions between polymer and enzyme. (C) 2003 Elsevier Ltd. All rights reserved.

AB - The application of biotransformations in solid phase synthesis is an attractive alternative to chemical methodologies. An important issue that needs to be addressed in this context is accessibility of functional groups within a porous polymer to the biocatalyst. This paper shows that such accessibility can be improved for penicillin G amidase by introducing permanent charges into the polymer. The effect appears to be due to better swelling of the polymer in buffer and to electrostatic interactions between polymer and enzyme. (C) 2003 Elsevier Ltd. All rights reserved.

KW - permanently charged groups

KW - PEGA resins

KW - improved biotransformations

KW - solid support

U2 - 10.1016/j.tet.2003.10.125

DO - 10.1016/j.tet.2003.10.125

M3 - Article

SN - 0040-4020

VL - 60

SP - 589

EP - 594

JO - Tetrahedron

JF - Tetrahedron

IS - 3

ER -

Introduction of permanently charged groups into PEGA resins leads to improved biotransformations on solid support

Abstract

Keywords

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