Introduction of permanently charged groups into PEGA resins leads to improved biotransformations on solid support

A Basso, R V Ulijn, S L Flitsch, G Margetts, I Brazendale, C Ebert, L De Martin, P Linda, S Verdelli, L Gardossi

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11 Citations (Scopus)

Abstract

The application of biotransformations in solid phase synthesis is an attractive alternative to chemical methodologies. An important issue that needs to be addressed in this context is accessibility of functional groups within a porous polymer to the biocatalyst. This paper shows that such accessibility can be improved for penicillin G amidase by introducing permanent charges into the polymer. The effect appears to be due to better swelling of the polymer in buffer and to electrostatic interactions between polymer and enzyme. (C) 2003 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)589-594
Number of pages6
JournalTetrahedron
Volume60
Issue number3
DOIs
Publication statusPublished - 12 Jan 2004

Keywords

  • permanently charged groups
  • PEGA resins
  • improved biotransformations
  • solid support

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    Basso, A., Ulijn, R. V., Flitsch, S. L., Margetts, G., Brazendale, I., Ebert, C., De Martin, L., Linda, P., Verdelli, S., & Gardossi, L. (2004). Introduction of permanently charged groups into PEGA resins leads to improved biotransformations on solid support. Tetrahedron, 60(3), 589-594. https://doi.org/10.1016/j.tet.2003.10.125