Intracellular S1P generation is essential for S1P-induced motility of human lung endothelial cells: role of sphingosine kinase 1 and S1P lyase

Evgeny V Berdyshev, Irina Gorshkova, Peter Usatyuk, Satish Kalari, Yutong Zhao, Nigel J Pyne, Susan Pyne, Roger A Sabbadini, Joe G.N. Garcia, Viswanathan Natarajan

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Earlier we have shown that extracellular sphingosine-1-phosphate (S1P) induces migration of human pulmonary artery endothelial cells (HPAECs) through the activation of S1P(1) receptor, PKCε, and PLD2-PKCζ-Rac1 signaling cascade. As endothelial cells generate intracellular S1P, here we have investigated the role of sphingosine kinases (SphKs) and S1P lyase (S1PL), that regulate intracellular S1P accumulation, in HPAEC motility.
LanguageEnglish
Pagese16571
JournalPLoS One
Volume6
Issue number1
DOIs
Publication statusPublished - 31 Jan 2011

Fingerprint

sphingosine
lyases
Endothelial cells
endothelial cells
phosphotransferases (kinases)
Endothelial Cells
lungs
phosphates
Lung
Pulmonary Artery
Lysosphingolipid Receptors
pulmonary artery
Cell Movement
Chemical activation
cell movement
sphingosine kinase
sphingosine 1-phosphate
sphingosine 1-phosphate lyase (aldolase)
receptors

Keywords

  • human lung endothelial cells
  • S1P generation
  • endothelial cell motility

Cite this

Berdyshev, Evgeny V ; Gorshkova, Irina ; Usatyuk, Peter ; Kalari, Satish ; Zhao, Yutong ; Pyne, Nigel J ; Pyne, Susan ; Sabbadini, Roger A ; Garcia, Joe G.N. ; Natarajan, Viswanathan. / Intracellular S1P generation is essential for S1P-induced motility of human lung endothelial cells : role of sphingosine kinase 1 and S1P lyase. In: PLoS One. 2011 ; Vol. 6, No. 1. pp. e16571.
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Intracellular S1P generation is essential for S1P-induced motility of human lung endothelial cells : role of sphingosine kinase 1 and S1P lyase. / Berdyshev, Evgeny V; Gorshkova, Irina; Usatyuk, Peter; Kalari, Satish; Zhao, Yutong; Pyne, Nigel J; Pyne, Susan; Sabbadini, Roger A; Garcia, Joe G.N.; Natarajan, Viswanathan.

In: PLoS One, Vol. 6, No. 1, 31.01.2011, p. e16571.

Research output: Contribution to journalArticle

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