Intracellular analysis by SERRS and nanoparticles

R. Stevenson; L. Senior; J . Alexander; M. Geboers; K. Faulds; D. Graham

Intracellular analysis by SERRS and nanoparticles

R. Stevenson, L. Senior, J . Alexander, M. Geboers, K. Faulds, D. Graham

Pure And Applied Chemistry

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution book

Abstract

We report the first use of ‘bare’ nanoparticles as both a cellular delivery vehicle and a spectroscopic detection platform to enable surface enhanced resonance Raman scattering (SERRS) detection. We have shown how this approach can be used to observe the conversion of a specific Raman active substrate whose enzyme is naturally occurring within that cell line. The technique has been used to pinpoint unique Raman signals at specific sites within a cell at nm resolution, suggesting a novel approach for enzyme localization and representing a major advancement upon both current SERRS and fluorescence cell based research. Further we show how addition of an enzyme inhibitor can be used to reduce the SERRS output in a dose dependant manner, a significant breakthrough upon current enzyme cellular analysis.

Original language	English
Title of host publication	Nanotechnology 2010
Subtitle of host publication	Bio Sensors, Instruments, Medical, Environment and Energy
Place of Publication	Austin, TX
Pages	153-156
Number of pages	4
Volume	3
Publication status	Published - 21 Jun 2010
Event	13th NSTI Nanotech 2010 Conference - Anaheim, California, , United States Duration: 21 Jun 2010 → 24 Jun 2010

Conference

Conference	13th NSTI Nanotech 2010 Conference
Country/Territory	United States
City	Anaheim, California,
Period	21/06/10 → 24/06/10

Keywords

SERRS
surface enhanced resonance Raman scattering
SERS
surface enhanced raman scattering
nanoparticles
intracellular detection
raman spectroscopy
molecular vibrations

Cite this

@inproceedings{cf11863d0149490c9d1f8634413a3cc6,

title = "Intracellular analysis by SERRS and nanoparticles",

abstract = "We report the first use of {\textquoteleft}bare{\textquoteright} nanoparticles as both a cellular delivery vehicle and a spectroscopic detection platform to enable surface enhanced resonance Raman scattering (SERRS) detection. We have shown how this approach can be used to observe the conversion of a specific Raman active substrate whose enzyme is naturally occurring within that cell line. The technique has been used to pinpoint unique Raman signals at specific sites within a cell at nm resolution, suggesting a novel approach for enzyme localization and representing a major advancement upon both current SERRS and fluorescence cell based research. Further we show how addition of an enzyme inhibitor can be used to reduce the SERRS output in a dose dependant manner, a significant breakthrough upon current enzyme cellular analysis.",

keywords = "SERRS, surface enhanced resonance Raman scattering , SERS, surface enhanced raman scattering, nanoparticles, intracellular detection, raman spectroscopy, molecular vibrations",

author = "R. Stevenson and L. Senior and Alexander, \{J .\} and M. Geboers and K. Faulds and D. Graham",

year = "2010",

month = jun,

day = "21",

language = "English",

isbn = "9781439834152",

volume = "3",

pages = "153--156",

booktitle = "Nanotechnology 2010",

note = "13th NSTI Nanotech 2010 Conference ; Conference date: 21-06-2010 Through 24-06-2010",

}

TY - GEN

T1 - Intracellular analysis by SERRS and nanoparticles

AU - Stevenson, R.

AU - Senior, L.

AU - Alexander, J .

AU - Geboers, M.

AU - Faulds, K.

AU - Graham, D.

PY - 2010/6/21

Y1 - 2010/6/21

N2 - We report the first use of ‘bare’ nanoparticles as both a cellular delivery vehicle and a spectroscopic detection platform to enable surface enhanced resonance Raman scattering (SERRS) detection. We have shown how this approach can be used to observe the conversion of a specific Raman active substrate whose enzyme is naturally occurring within that cell line. The technique has been used to pinpoint unique Raman signals at specific sites within a cell at nm resolution, suggesting a novel approach for enzyme localization and representing a major advancement upon both current SERRS and fluorescence cell based research. Further we show how addition of an enzyme inhibitor can be used to reduce the SERRS output in a dose dependant manner, a significant breakthrough upon current enzyme cellular analysis.

AB - We report the first use of ‘bare’ nanoparticles as both a cellular delivery vehicle and a spectroscopic detection platform to enable surface enhanced resonance Raman scattering (SERRS) detection. We have shown how this approach can be used to observe the conversion of a specific Raman active substrate whose enzyme is naturally occurring within that cell line. The technique has been used to pinpoint unique Raman signals at specific sites within a cell at nm resolution, suggesting a novel approach for enzyme localization and representing a major advancement upon both current SERRS and fluorescence cell based research. Further we show how addition of an enzyme inhibitor can be used to reduce the SERRS output in a dose dependant manner, a significant breakthrough upon current enzyme cellular analysis.

KW - SERRS

KW - surface enhanced resonance Raman scattering

KW - SERS

KW - surface enhanced raman scattering

KW - nanoparticles

KW - intracellular detection

KW - raman spectroscopy

KW - molecular vibrations

UR - http://www.techconnectworld.com/Nanotech2010/

UR - http://www.nsti.org/procs/Nanotech2010v3/1/W50.126

M3 - Conference contribution book

SN - 9781439834152

VL - 3

SP - 153

EP - 156

BT - Nanotechnology 2010

CY - Austin, TX

T2 - 13th NSTI Nanotech 2010 Conference

Y2 - 21 June 2010 through 24 June 2010

ER -

Intracellular analysis by SERRS and nanoparticles

Abstract

Conference

Keywords

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