Intracellular analysis by SERRS and nanoparticles

R. Stevenson, L. Senior, J . Alexander, M. Geboers, K. Faulds, D. Graham

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

Abstract

We report the first use of ‘bare’ nanoparticles as both a cellular delivery vehicle and a spectroscopic detection platform to enable surface enhanced resonance Raman scattering (SERRS) detection. We have shown how this approach can be used to observe the conversion of a specific Raman active substrate whose enzyme is naturally occurring within that cell line. The technique has been used to pinpoint unique Raman signals at specific sites within a cell at nm resolution, suggesting a novel approach for enzyme localization and representing a major advancement upon both current SERRS and fluorescence cell based research. Further we show how addition of an enzyme inhibitor can be used to reduce the SERRS output in a dose dependant manner, a significant breakthrough upon current enzyme cellular analysis.
Original languageEnglish
Title of host publicationNanotechnology 2010
Subtitle of host publicationBio Sensors, Instruments, Medical, Environment and Energy
Place of PublicationAustin, TX
Pages153-156
Number of pages4
Volume3
Publication statusPublished - 21 Jun 2010
Event13th NSTI Nanotech 2010 Conference - Anaheim, California, , United States
Duration: 21 Jun 201024 Jun 2010

Conference

Conference13th NSTI Nanotech 2010 Conference
CountryUnited States
CityAnaheim, California,
Period21/06/1024/06/10

Keywords

  • SERRS
  • surface enhanced resonance Raman scattering
  • SERS
  • surface enhanced raman scattering
  • nanoparticles
  • intracellular detection
  • raman spectroscopy
  • molecular vibrations

Cite this

Stevenson, R., Senior, L., Alexander, J. ., Geboers, M., Faulds, K., & Graham, D. (2010). Intracellular analysis by SERRS and nanoparticles. In Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy (Vol. 3, pp. 153-156).