Intracellular analysis by SERRS and nanoparticles

R. Stevenson, L. Senior, J . Alexander, M. Geboers, K. Faulds, D. Graham

Research output: Chapter in Book/Report/Conference proceedingConference contribution book


We report the first use of ‘bare’ nanoparticles as both a cellular delivery vehicle and a spectroscopic detection platform to enable surface enhanced resonance Raman scattering (SERRS) detection. We have shown how this approach can be used to observe the conversion of a specific Raman active substrate whose enzyme is naturally occurring within that cell line. The technique has been used to pinpoint unique Raman signals at specific sites within a cell at nm resolution, suggesting a novel approach for enzyme localization and representing a major advancement upon both current SERRS and fluorescence cell based research. Further we show how addition of an enzyme inhibitor can be used to reduce the SERRS output in a dose dependant manner, a significant breakthrough upon current enzyme cellular analysis.
Original languageEnglish
Title of host publicationNanotechnology 2010
Subtitle of host publicationBio Sensors, Instruments, Medical, Environment and Energy
Place of PublicationAustin, TX
Number of pages4
Publication statusPublished - 21 Jun 2010
Event13th NSTI Nanotech 2010 Conference - Anaheim, California, , United States
Duration: 21 Jun 201024 Jun 2010


Conference13th NSTI Nanotech 2010 Conference
Country/TerritoryUnited States
CityAnaheim, California,


  • surface enhanced resonance Raman scattering
  • SERS
  • surface enhanced raman scattering
  • nanoparticles
  • intracellular detection
  • raman spectroscopy
  • molecular vibrations


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