Intracellular analysis by SERRS and nanoparticles

R. Stevenson, L. Senior, J . Alexander, M. Geboers, K. Faulds, D. Graham

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

Abstract

We report the first use of ‘bare’ nanoparticles as both a cellular delivery vehicle and a spectroscopic detection platform to enable surface enhanced resonance Raman scattering (SERRS) detection. We have shown how this approach can be used to observe the conversion of a specific Raman active substrate whose enzyme is naturally occurring within that cell line. The technique has been used to pinpoint unique Raman signals at specific sites within a cell at nm resolution, suggesting a novel approach for enzyme localization and representing a major advancement upon both current SERRS and fluorescence cell based research. Further we show how addition of an enzyme inhibitor can be used to reduce the SERRS output in a dose dependant manner, a significant breakthrough upon current enzyme cellular analysis.
LanguageEnglish
Title of host publicationNanotechnology 2010
Subtitle of host publicationBio Sensors, Instruments, Medical, Environment and Energy
Place of PublicationAustin, TX
Pages153-156
Number of pages4
Volume3
Publication statusPublished - 21 Jun 2010
Event13th NSTI Nanotech 2010 Conference - Anaheim, California, , United States
Duration: 21 Jun 201024 Jun 2010

Conference

Conference13th NSTI Nanotech 2010 Conference
CountryUnited States
CityAnaheim, California,
Period21/06/1024/06/10

Fingerprint

Raman Spectrum Analysis
Nanoparticles
Enzymes
Enzyme Inhibitors
Fluorescence
Cell Line
Research

Keywords

  • SERRS
  • surface enhanced resonance Raman scattering
  • SERS
  • surface enhanced raman scattering
  • nanoparticles
  • intracellular detection
  • raman spectroscopy
  • molecular vibrations

Cite this

Stevenson, R., Senior, L., Alexander, J. ., Geboers, M., Faulds, K., & Graham, D. (2010). Intracellular analysis by SERRS and nanoparticles. In Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy (Vol. 3, pp. 153-156). Austin, TX.
Stevenson, R. ; Senior, L. ; Alexander, J . ; Geboers, M. ; Faulds, K. ; Graham, D. / Intracellular analysis by SERRS and nanoparticles. Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy. Vol. 3 Austin, TX, 2010. pp. 153-156
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abstract = "We report the first use of ‘bare’ nanoparticles as both a cellular delivery vehicle and a spectroscopic detection platform to enable surface enhanced resonance Raman scattering (SERRS) detection. We have shown how this approach can be used to observe the conversion of a specific Raman active substrate whose enzyme is naturally occurring within that cell line. The technique has been used to pinpoint unique Raman signals at specific sites within a cell at nm resolution, suggesting a novel approach for enzyme localization and representing a major advancement upon both current SERRS and fluorescence cell based research. Further we show how addition of an enzyme inhibitor can be used to reduce the SERRS output in a dose dependant manner, a significant breakthrough upon current enzyme cellular analysis.",
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Stevenson, R, Senior, L, Alexander, J, Geboers, M, Faulds, K & Graham, D 2010, Intracellular analysis by SERRS and nanoparticles. in Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy. vol. 3, Austin, TX, pp. 153-156, 13th NSTI Nanotech 2010 Conference , Anaheim, California, , United States, 21/06/10.

Intracellular analysis by SERRS and nanoparticles. / Stevenson, R.; Senior, L.; Alexander, J .; Geboers, M.; Faulds, K.; Graham, D.

Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy. Vol. 3 Austin, TX, 2010. p. 153-156.

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

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T1 - Intracellular analysis by SERRS and nanoparticles

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AU - Senior, L.

AU - Alexander, J .

AU - Geboers, M.

AU - Faulds, K.

AU - Graham, D.

PY - 2010/6/21

Y1 - 2010/6/21

N2 - We report the first use of ‘bare’ nanoparticles as both a cellular delivery vehicle and a spectroscopic detection platform to enable surface enhanced resonance Raman scattering (SERRS) detection. We have shown how this approach can be used to observe the conversion of a specific Raman active substrate whose enzyme is naturally occurring within that cell line. The technique has been used to pinpoint unique Raman signals at specific sites within a cell at nm resolution, suggesting a novel approach for enzyme localization and representing a major advancement upon both current SERRS and fluorescence cell based research. Further we show how addition of an enzyme inhibitor can be used to reduce the SERRS output in a dose dependant manner, a significant breakthrough upon current enzyme cellular analysis.

AB - We report the first use of ‘bare’ nanoparticles as both a cellular delivery vehicle and a spectroscopic detection platform to enable surface enhanced resonance Raman scattering (SERRS) detection. We have shown how this approach can be used to observe the conversion of a specific Raman active substrate whose enzyme is naturally occurring within that cell line. The technique has been used to pinpoint unique Raman signals at specific sites within a cell at nm resolution, suggesting a novel approach for enzyme localization and representing a major advancement upon both current SERRS and fluorescence cell based research. Further we show how addition of an enzyme inhibitor can be used to reduce the SERRS output in a dose dependant manner, a significant breakthrough upon current enzyme cellular analysis.

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KW - surface enhanced resonance Raman scattering

KW - SERS

KW - surface enhanced raman scattering

KW - nanoparticles

KW - intracellular detection

KW - raman spectroscopy

KW - molecular vibrations

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M3 - Conference contribution book

SN - 9781439834152

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BT - Nanotechnology 2010

CY - Austin, TX

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Stevenson R, Senior L, Alexander J, Geboers M, Faulds K, Graham D. Intracellular analysis by SERRS and nanoparticles. In Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy. Vol. 3. Austin, TX. 2010. p. 153-156