Projects per year
Nutrient-transporting channels have been recently discovered in mature Escherichia coli biofilms, however the relationship between intra-colony channel structure and the surrounding environmental conditions is poorly understood. Using a combination of fluorescence mesoscopy and a purpose-designed open-source quantitative image analysis pipeline, we show that growth substrate composition and nutrient availability have a profound effect on the morphology of intra-colony channels in mature E. coli biofilms. Under all nutrient conditions, intra-colony channel width was observed to increase non-linearly with radial distance from the centre of the biofilm. Notably, the channels were around 25% wider at the centre of carbon-limited biofilms compared to nitrogen-limited biofilms. Channel density also differed in colonies grown on rich and minimal media, with the former creating a network of tightly packed channels and the latter leading to well-separated, wider channels with defined edges. Our approach paves the way for measurement of internal patterns in a wide range of biofilms, offering the potential for new insights into infection and pathogenicity.
|Number of pages||14|
|Early online date||13 Oct 2022|
|Publication status||Published - 31 Dec 2022|
- intra-colony channel morphology
- escherichia coli
- nutrient availability
- substrate stiffness
FingerprintDive into the research topics of 'Intra-colony channel morphology in Escherichia coli biofilms is governed by nutrient availability and substrate stiffness'. Together they form a unique fingerprint.
- 4 Finished