Intestinal pathology during acute toxoplasmosis is IL-4 dependent and unrelated to parasite burden

M. B. Nickdel; R. E. Lyons; F. Roberts; F. Brombacher; C. A. Hunter; J. Alexander; C. W. Roberts

doi:10.1111/j.0141-9838.2004.00686.x

Intestinal pathology during acute toxoplasmosis is IL-4 dependent and unrelated to parasite burden

M. B. Nickdel, R. E. Lyons, F. Roberts, F. Brombacher, C. A. Hunter, J. Alexander, C. W. Roberts

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24 Citations (Scopus)

Abstract

The role of interleukin-4 (IL-4) during the course of Toxoplasma gondii infection was studied using IL-4-/- mice and their wild-type (WT) counterparts on a C57BL/6 background. Following oral infection with T. gondii tissue cysts an exacerbative role for IL-4 was demonstrated and IL-4-/- mice were found to be more resistant to infection than WT mice as measured by significantly reduced mortality. Furthermore pathology in the small intestine was less severe in IL-4-/- mice although conversely liver pathology was greater than in wild-type mice. Significantly, plasma IL-12 and IFN-gamma levels, which peaked at days 6 and 8, respectively, were higher in IL-4-/- mice. The exacerbatory role of IL-4 in the intestine was found by competitive RT-PCR not to be associated with increased parasite burdens but was related to comparative expression of IL-10.

Original language	English
Pages (from-to)	75-82
Number of pages	8
Journal	Parasite Immunology
Volume	26
Issue number	2
DOIs	https://doi.org/10.1111/j.0141-9838.2004.00686.x
Publication status	Published - 29 Feb 2004

Keywords

acute disease
animals
cytokines
female
interleukin-4
intestine, small
male
mice
mice, inbred C57BL
Th1 cells
Th2 cells
toxoplasma
toxoplasmosis, animal

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10.1111/j.0141-9838.2004.00686.x

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title = "Intestinal pathology during acute toxoplasmosis is IL-4 dependent and unrelated to parasite burden",

abstract = "The role of interleukin-4 (IL-4) during the course of Toxoplasma gondii infection was studied using IL-4-/- mice and their wild-type (WT) counterparts on a C57BL/6 background. Following oral infection with T. gondii tissue cysts an exacerbative role for IL-4 was demonstrated and IL-4-/- mice were found to be more resistant to infection than WT mice as measured by significantly reduced mortality. Furthermore pathology in the small intestine was less severe in IL-4-/- mice although conversely liver pathology was greater than in wild-type mice. Significantly, plasma IL-12 and IFN-gamma levels, which peaked at days 6 and 8, respectively, were higher in IL-4-/- mice. The exacerbatory role of IL-4 in the intestine was found by competitive RT-PCR not to be associated with increased parasite burdens but was related to comparative expression of IL-10.",

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author = "Nickdel, {M. B.} and Lyons, {R. E.} and F. Roberts and F. Brombacher and Hunter, {C. A.} and J. Alexander and Roberts, {C. W.}",

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AU - Nickdel, M. B.

AU - Lyons, R. E.

AU - Roberts, F.

AU - Brombacher, F.

AU - Hunter, C. A.

AU - Alexander, J.

AU - Roberts, C. W.

PY - 2004/2/29

Y1 - 2004/2/29

N2 - The role of interleukin-4 (IL-4) during the course of Toxoplasma gondii infection was studied using IL-4-/- mice and their wild-type (WT) counterparts on a C57BL/6 background. Following oral infection with T. gondii tissue cysts an exacerbative role for IL-4 was demonstrated and IL-4-/- mice were found to be more resistant to infection than WT mice as measured by significantly reduced mortality. Furthermore pathology in the small intestine was less severe in IL-4-/- mice although conversely liver pathology was greater than in wild-type mice. Significantly, plasma IL-12 and IFN-gamma levels, which peaked at days 6 and 8, respectively, were higher in IL-4-/- mice. The exacerbatory role of IL-4 in the intestine was found by competitive RT-PCR not to be associated with increased parasite burdens but was related to comparative expression of IL-10.

AB - The role of interleukin-4 (IL-4) during the course of Toxoplasma gondii infection was studied using IL-4-/- mice and their wild-type (WT) counterparts on a C57BL/6 background. Following oral infection with T. gondii tissue cysts an exacerbative role for IL-4 was demonstrated and IL-4-/- mice were found to be more resistant to infection than WT mice as measured by significantly reduced mortality. Furthermore pathology in the small intestine was less severe in IL-4-/- mice although conversely liver pathology was greater than in wild-type mice. Significantly, plasma IL-12 and IFN-gamma levels, which peaked at days 6 and 8, respectively, were higher in IL-4-/- mice. The exacerbatory role of IL-4 in the intestine was found by competitive RT-PCR not to be associated with increased parasite burdens but was related to comparative expression of IL-10.

KW - acute disease

KW - animals

KW - cytokines

KW - female

KW - interleukin-4

KW - intestine, small

KW - male

KW - mice

KW - mice, inbred C57BL

KW - Th1 cells

KW - Th2 cells

KW - toxoplasma

KW - toxoplasmosis, animal

UR - http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-3024

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DO - 10.1111/j.0141-9838.2004.00686.x

M3 - Article

C2 - 15225294

SN - 0141-9838

VL - 26

SP - 75

EP - 82

JO - Parasite Immunology

JF - Parasite Immunology

IS - 2

ER -

Intestinal pathology during acute toxoplasmosis is IL-4 dependent and unrelated to parasite burden

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Keywords

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