Internal labeling of oligonucleotide probes by Diels-Alder cycloaddition

D. Graham, A. Grondin, C. McHugh, L. Fruk, W.E. Smith

Research output: Contribution to journalArticle

36 Citations (Scopus)


A new method of adding fluorescent labels to the middle of oligonucleotides is reported. Diets-Alder cycloaddition was used to add five fluorescent maleimides to an oligonucleotide containing a 2'-deoxyuridine modified at the 5-position with a spaced furan. This is a new approach to internal oligonucleotide chemistry that opens Lip a large range of possibilities for further conjugation.
Original languageEnglish
Pages (from-to)4785-4788
Number of pages3
JournalTetrahedron Letters
Issue number27
Publication statusPublished - 1 Jul 2002


  • dna
  • oligonucleotide probes
  • cycloaddition

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