A new method of adding fluorescent labels to the middle of oligonucleotides is reported. Diets-Alder cycloaddition was used to add five fluorescent maleimides to an oligonucleotide containing a 2'-deoxyuridine modified at the 5-position with a spaced furan. This is a new approach to internal oligonucleotide chemistry that opens Lip a large range of possibilities for further conjugation.
- oligonucleotide probes
Graham, D., Grondin, A., McHugh, C., Fruk, L., & Smith, W. E. (2002). Internal labeling of oligonucleotide probes by Diels-Alder cycloaddition. Tetrahedron Letters, 43(27), 4785-4788. https://doi.org/10.1016/S0040-4039(02)00930-9