Internal labeling of oligonucleotide probes by Diels-Alder cycloaddition

D. Graham, A. Grondin, C. McHugh, L. Fruk, W.E. Smith

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

A new method of adding fluorescent labels to the middle of oligonucleotides is reported. Diets-Alder cycloaddition was used to add five fluorescent maleimides to an oligonucleotide containing a 2'-deoxyuridine modified at the 5-position with a spaced furan. This is a new approach to internal oligonucleotide chemistry that opens Lip a large range of possibilities for further conjugation.
LanguageEnglish
Pages4785-4788
Number of pages3
JournalTetrahedron Letters
Volume43
Issue number27
DOIs
Publication statusPublished - 1 Jul 2002

Fingerprint

Cycloaddition
Oligonucleotide Probes
Cycloaddition Reaction
Oligonucleotides
Labeling
Maleimides
Deoxyuridine
Nutrition
Lip
Labels
Diet

Keywords

  • dna
  • oligonucleotide probes
  • cycloaddition

Cite this

Graham, D. ; Grondin, A. ; McHugh, C. ; Fruk, L. ; Smith, W.E. / Internal labeling of oligonucleotide probes by Diels-Alder cycloaddition. In: Tetrahedron Letters. 2002 ; Vol. 43, No. 27. pp. 4785-4788.
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Internal labeling of oligonucleotide probes by Diels-Alder cycloaddition. / Graham, D.; Grondin, A.; McHugh, C.; Fruk, L.; Smith, W.E.

In: Tetrahedron Letters, Vol. 43, No. 27, 01.07.2002, p. 4785-4788.

Research output: Contribution to journalArticle

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