Internal labeling of oligonucleotide probes by Diels-Alder cycloaddition

D. Graham; A. Grondin; C. McHugh; L. Fruk; W.E. Smith

doi:10.1016/S0040-4039(02)00930-9

Internal labeling of oligonucleotide probes by Diels-Alder cycloaddition

D. Graham, A. Grondin, C. McHugh, L. Fruk, W.E. Smith

Pure And Applied Chemistry

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

A new method of adding fluorescent labels to the middle of oligonucleotides is reported. Diets-Alder cycloaddition was used to add five fluorescent maleimides to an oligonucleotide containing a 2'-deoxyuridine modified at the 5-position with a spaced furan. This is a new approach to internal oligonucleotide chemistry that opens Lip a large range of possibilities for further conjugation.

Original language	English
Pages (from-to)	4785-4788
Number of pages	3
Journal	Tetrahedron Letters
Volume	43
Issue number	27
DOIs	https://doi.org/10.1016/S0040-4039(02)00930-9
Publication status	Published - 1 Jul 2002

Keywords

dna
oligonucleotide probes
cycloaddition

Access to Document

10.1016/S0040-4039(02)00930-9

Cite this

@article{dc8e003509ed4abd9e98d5b7ae6e446d,

title = "Internal labeling of oligonucleotide probes by Diels-Alder cycloaddition",

abstract = "A new method of adding fluorescent labels to the middle of oligonucleotides is reported. Diets-Alder cycloaddition was used to add five fluorescent maleimides to an oligonucleotide containing a 2'-deoxyuridine modified at the 5-position with a spaced furan. This is a new approach to internal oligonucleotide chemistry that opens Lip a large range of possibilities for further conjugation.",

keywords = "dna, oligonucleotide probes, cycloaddition",

author = "D. Graham and A. Grondin and C. McHugh and L. Fruk and W.E. Smith",

year = "2002",

month = jul,

day = "1",

doi = "10.1016/S0040-4039(02)00930-9",

language = "English",

volume = "43",

pages = "4785--4788",

journal = "Tetrahedron Letters",

issn = "0040-4039",

publisher = "Elsevier Limited",

number = "27",

}

TY - JOUR

T1 - Internal labeling of oligonucleotide probes by Diels-Alder cycloaddition

AU - Graham, D.

AU - Grondin, A.

AU - McHugh, C.

AU - Fruk, L.

AU - Smith, W.E.

PY - 2002/7/1

Y1 - 2002/7/1

N2 - A new method of adding fluorescent labels to the middle of oligonucleotides is reported. Diets-Alder cycloaddition was used to add five fluorescent maleimides to an oligonucleotide containing a 2'-deoxyuridine modified at the 5-position with a spaced furan. This is a new approach to internal oligonucleotide chemistry that opens Lip a large range of possibilities for further conjugation.

AB - A new method of adding fluorescent labels to the middle of oligonucleotides is reported. Diets-Alder cycloaddition was used to add five fluorescent maleimides to an oligonucleotide containing a 2'-deoxyuridine modified at the 5-position with a spaced furan. This is a new approach to internal oligonucleotide chemistry that opens Lip a large range of possibilities for further conjugation.

KW - dna

KW - oligonucleotide probes

KW - cycloaddition

UR - http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6THS-45X09B9-9-B&_cdi=5290&_user=875629&_orig=search&_coverDate=07%2F01%2F2002&_qd=1&_sk=999569972&view=c&wchp=dGLbVtb-zSkWW&md5=212b1181974e3db5eb4d94c2fa1f287c&ie=/sdarticle.pdf

UR - http://dx.doi.org/10.1016/S0040-4039(02)00930-9

U2 - 10.1016/S0040-4039(02)00930-9

DO - 10.1016/S0040-4039(02)00930-9

M3 - Article

SN - 0040-4039

VL - 43

SP - 4785

EP - 4788

JO - Tetrahedron Letters

JF - Tetrahedron Letters

IS - 27

ER -

Internal labeling of oligonucleotide probes by Diels-Alder cycloaddition

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this