Interference reflection microscopy shows novel insights to bacterial gliding motility

Liam M. Rooney; Paul A. Hoskisson; Gail McConnell

Interference reflection microscopy shows novel insights to bacterial gliding motility

Liam M. Rooney, Paul A. Hoskisson, Gail McConnell

Research output: Contribution to conference › Poster

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Abstract

The gliding motility of the Δ-proteobacterium, Myxococcus xanthus is used to facilitate either social or adventurous motility depending on the availability of nutrients in their environment. The size of bacteria limits our ability to use sectioning microscopy techniques, and so most studies on gliding motility use fluorescence-based techniques to focus on lateral (x, y) dynamics. We aim to use interference reflection microscopy (IRM) to visualise the axial motility dynamics in gliding cells to better understand their underlying gliding motility mechanisms.

Original language	English
Number of pages	1
Publication status	Published - 5 Jun 2018
Event	18th European Light Microscopy Initiative Meeting 2018 - Dublin, Ireland Duration: 5 Jun 2018 → 8 Jun 2018 Conference number: 18th https://www.elmi2018.eu/

Conference

Conference	18th European Light Microscopy Initiative Meeting 2018
Abbreviated title	ELMI 2018
Country/Territory	Ireland
City	Dublin
Period	5/06/18 → 8/06/18
Internet address	https://www.elmi2018.eu/

Keywords

myxococcus xanthus
biomedical imaging
microbiology

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Rooney-etal-ELMI-2018-Interference-reflection-microscopy-shows-novel-insights-to-bacterialAccepted author manuscript, 16.7 MB

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title = "Interference reflection microscopy shows novel insights to bacterial gliding motility",

abstract = "The gliding motility of the Δ-proteobacterium, Myxococcus xanthus is used to facilitate either social or adventurous motility depending on the availability of nutrients in their environment. The size of bacteria limits our ability to use sectioning microscopy techniques, and so most studies on gliding motility use fluorescence-based techniques to focus on lateral (x, y) dynamics. We aim to use interference reflection microscopy (IRM) to visualise the axial motility dynamics in gliding cells to better understand their underlying gliding motility mechanisms.",

keywords = "myxococcus xanthus, biomedical imaging, microbiology",

author = "Rooney, {Liam M.} and Hoskisson, {Paul A.} and Gail McConnell",

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T1 - Interference reflection microscopy shows novel insights to bacterial gliding motility

AU - Rooney, Liam M.

AU - Hoskisson, Paul A.

AU - McConnell, Gail

N1 - Conference code: 18th

PY - 2018/6/5

Y1 - 2018/6/5

N2 - The gliding motility of the Δ-proteobacterium, Myxococcus xanthus is used to facilitate either social or adventurous motility depending on the availability of nutrients in their environment. The size of bacteria limits our ability to use sectioning microscopy techniques, and so most studies on gliding motility use fluorescence-based techniques to focus on lateral (x, y) dynamics. We aim to use interference reflection microscopy (IRM) to visualise the axial motility dynamics in gliding cells to better understand their underlying gliding motility mechanisms.

AB - The gliding motility of the Δ-proteobacterium, Myxococcus xanthus is used to facilitate either social or adventurous motility depending on the availability of nutrients in their environment. The size of bacteria limits our ability to use sectioning microscopy techniques, and so most studies on gliding motility use fluorescence-based techniques to focus on lateral (x, y) dynamics. We aim to use interference reflection microscopy (IRM) to visualise the axial motility dynamics in gliding cells to better understand their underlying gliding motility mechanisms.

KW - myxococcus xanthus

KW - biomedical imaging

KW - microbiology

UR - https://www.elmi2018.eu/

M3 - Poster

T2 - 18th European Light Microscopy Initiative Meeting 2018

Y2 - 5 June 2018 through 8 June 2018

ER -

Interference reflection microscopy shows novel insights to bacterial gliding motility

Abstract

Conference

Keywords

Access to Document

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Supplementary information for: "Three-Dimensional Observations of an Aperiodic Oscillatory Gliding Behavior in Myxococcus xanthus Using Confocal Interference Reflection Microscopy"

Cite this

Interference reflection microscopy shows novel insights to bacterial gliding motility

Abstract

Conference

Keywords

Access to Document

Other files and links

Fingerprint

Datasets

Supplementary information for: "Three-Dimensional Observations of an Aperiodic Oscillatory Gliding Behavior in Myxococcus xanthus Using Confocal Interference Reflection Microscopy"

Cite this