Interference reflection microscopy shows novel insights to bacterial gliding motility

Liam M. Rooney; Paul A. Hoskisson; Gail McConnell

Interference reflection microscopy shows novel insights to bacterial gliding motility

Liam M. Rooney, Paul A. Hoskisson, Gail McConnell

Research output: Contribution to conference › Poster

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Abstract

The gliding motility of the Δ-proteobacterium, Myxococcus xanthus is used to facilitate either social or adventurous motility depending on the availability of nutrients in their environment. The size of bacteria limits our ability to use sectioning microscopy techniques, and so most studies on gliding motility use fluorescence-based techniques to focus on lateral (x, y) dynamics. We aim to use interference reflection microscopy (IRM) to visualise the axial motility dynamics in gliding cells to better understand their underlying gliding motility mechanisms.

Original language	English
Number of pages	1
Publication status	Published - 14 Mar 2018
Event	Spatiotemporal Organization of Bacterial Cells - Marburg, Germany Duration: 14 Mar 2018 → 16 Mar 2018

Conference

Conference	Spatiotemporal Organization of Bacterial Cells
Country/Territory	Germany
City	Marburg
Period	14/03/18 → 16/03/18

Keywords

microbiology
biomedical imaging
interference reflection microscopy
myxococcus xanthus

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Rooney-etal-SOBS-2018-Interference-reflection-microscopy-shows-novel-insights-to-bacterialAccepted author manuscript, 16.7 MB

Supplementary information for: "Three-Dimensional Observations of an Aperiodic Oscillatory Gliding Behavior in Myxococcus xanthus Using Confocal Interference Reflection Microscopy"
Rooney, L. (Creator) & McConnell, G. (Creator), University of Strathclyde, 21 Feb 2020
DOI: 10.15129/8a0a0e89-847e-4950-9535-67742d92ae25
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title = "Interference reflection microscopy shows novel insights to bacterial gliding motility",

abstract = "The gliding motility of the Δ-proteobacterium, Myxococcus xanthus is used to facilitate either social or adventurous motility depending on the availability of nutrients in their environment. The size of bacteria limits our ability to use sectioning microscopy techniques, and so most studies on gliding motility use fluorescence-based techniques to focus on lateral (x, y) dynamics. We aim to use interference reflection microscopy (IRM) to visualise the axial motility dynamics in gliding cells to better understand their underlying gliding motility mechanisms.",

keywords = "microbiology, biomedical imaging, interference reflection microscopy, myxococcus xanthus",

author = "Rooney, {Liam M.} and Hoskisson, {Paul A.} and Gail McConnell",

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note = "Spatiotemporal Organization of Bacterial Cells ; Conference date: 14-03-2018 Through 16-03-2018",

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T1 - Interference reflection microscopy shows novel insights to bacterial gliding motility

AU - Rooney, Liam M.

AU - Hoskisson, Paul A.

AU - McConnell, Gail

PY - 2018/3/14

Y1 - 2018/3/14

N2 - The gliding motility of the Δ-proteobacterium, Myxococcus xanthus is used to facilitate either social or adventurous motility depending on the availability of nutrients in their environment. The size of bacteria limits our ability to use sectioning microscopy techniques, and so most studies on gliding motility use fluorescence-based techniques to focus on lateral (x, y) dynamics. We aim to use interference reflection microscopy (IRM) to visualise the axial motility dynamics in gliding cells to better understand their underlying gliding motility mechanisms.

AB - The gliding motility of the Δ-proteobacterium, Myxococcus xanthus is used to facilitate either social or adventurous motility depending on the availability of nutrients in their environment. The size of bacteria limits our ability to use sectioning microscopy techniques, and so most studies on gliding motility use fluorescence-based techniques to focus on lateral (x, y) dynamics. We aim to use interference reflection microscopy (IRM) to visualise the axial motility dynamics in gliding cells to better understand their underlying gliding motility mechanisms.

KW - microbiology

KW - biomedical imaging

KW - interference reflection microscopy

KW - myxococcus xanthus

M3 - Poster

T2 - Spatiotemporal Organization of Bacterial Cells

Y2 - 14 March 2018 through 16 March 2018

ER -

Interference reflection microscopy shows novel insights to bacterial gliding motility

Abstract

Conference

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Supplementary information for: "Three-Dimensional Observations of an Aperiodic Oscillatory Gliding Behavior in Myxococcus xanthus Using Confocal Interference Reflection Microscopy"

Cite this