Interference reflection microscopy shows novel insights to bacterial gliding motility

Liam M. Rooney; Paul A. Hoskisson; Gail McConnell

Interference reflection microscopy shows novel insights to bacterial gliding motility

Liam M. Rooney, Paul A. Hoskisson, Gail McConnell

Research output: Contribution to conference › Poster › peer-review

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Abstract

The gliding motility of the Δ-proteobacterium, Myxococcus xanthus is used to facilitate either social or adventurous motility depending on the availability of nutrients in their environment. The size of bacteria limits our ability to use sectioning microscopy techniques, and so most studies on gliding motility use fluorescence-based techniques to focus on lateral ( x , y ) dynamics. We aim to use interference reflection microscopy (IRM) to visualise the axial motility dynamics in gliding cells to better understand their underlying gliding motility mechanisms.

Original language	English
Publication status	Published - 4 Apr 2018
Event	EMBO Practical Course on Advanced Optical Microscopy for Cell Biology - The Marine Biological Association, Plymouth, United Kingdom Duration: 4 Apr 2018 → 14 Apr 2018 Conference number: 15

Workshop

Workshop	EMBO Practical Course on Advanced Optical Microscopy for Cell Biology
Country/Territory	United Kingdom
City	Plymouth
Period	4/04/18 → 14/04/18

Keywords

interference reflection microscopy
optical microscopy
microbiology
biomedical Imaging
Myxococcus xanthus

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Rooney-etal-EMBO2018-Interference-reflection-microscopy-shows-novel-insightsFinal published version, 3.38 MB

Supplementary information for: "Three-Dimensional Observations of an Aperiodic Oscillatory Gliding Behavior in Myxococcus xanthus Using Confocal Interference Reflection Microscopy"
Rooney, L. (Creator) & McConnell, G. (Creator), University of Strathclyde, 21 Feb 2020
DOI: 10.15129/8a0a0e89-847e-4950-9535-67742d92ae25
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title = "Interference reflection microscopy shows novel insights to bacterial gliding motility",

abstract = "The gliding motility of the Δ-proteobacterium, Myxococcus xanthus is used to facilitate either social or adventurous motility depending on the availability of nutrients in their environment. The size of bacteria limits our ability to use sectioning microscopy techniques, and so most studies on gliding motility use fluorescence-based techniques to focus on lateral ( x , y ) dynamics. We aim to use interference reflection microscopy (IRM) to visualise the axial motility dynamics in gliding cells to better understand their underlying gliding motility mechanisms.",

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author = "Rooney, {Liam M.} and Hoskisson, {Paul A.} and Gail McConnell",

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language = "English",

note = "EMBO Practical Course on Advanced Optical Microscopy for Cell Biology ; Conference date: 04-04-2018 Through 14-04-2018",

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T1 - Interference reflection microscopy shows novel insights to bacterial gliding motility

AU - Rooney, Liam M.

AU - Hoskisson, Paul A.

AU - McConnell, Gail

N1 - Conference code: 15

PY - 2018/4/4

Y1 - 2018/4/4

N2 - The gliding motility of the Δ-proteobacterium, Myxococcus xanthus is used to facilitate either social or adventurous motility depending on the availability of nutrients in their environment. The size of bacteria limits our ability to use sectioning microscopy techniques, and so most studies on gliding motility use fluorescence-based techniques to focus on lateral ( x , y ) dynamics. We aim to use interference reflection microscopy (IRM) to visualise the axial motility dynamics in gliding cells to better understand their underlying gliding motility mechanisms.

AB - The gliding motility of the Δ-proteobacterium, Myxococcus xanthus is used to facilitate either social or adventurous motility depending on the availability of nutrients in their environment. The size of bacteria limits our ability to use sectioning microscopy techniques, and so most studies on gliding motility use fluorescence-based techniques to focus on lateral ( x , y ) dynamics. We aim to use interference reflection microscopy (IRM) to visualise the axial motility dynamics in gliding cells to better understand their underlying gliding motility mechanisms.

KW - interference reflection microscopy

KW - optical microscopy

KW - microbiology

KW - biomedical Imaging

KW - Myxococcus xanthus

M3 - Poster

T2 - EMBO Practical Course on Advanced Optical Microscopy for Cell Biology

Y2 - 4 April 2018 through 14 April 2018

ER -

Interference reflection microscopy shows novel insights to bacterial gliding motility

Abstract

Workshop

Keywords

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Supplementary information for: "Three-Dimensional Observations of an Aperiodic Oscillatory Gliding Behavior in Myxococcus xanthus Using Confocal Interference Reflection Microscopy"

Cite this