Interfacing biodegradable molecular hydrogels with liquid crystals

I-Hsin Lin, Louise S. Birchall, Nigel Hodson, Rein V. Ulijn, Simon J. Webb

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A self-assembled Fmoc-peptide hydrogel has been interfaced with a liquid crystal (LC) display to give an optical sensor for enzyme activity. An Fmoc-TL-OMe hydrogel was selected as it can be formed in situ by enzyme-mediated assembly with thermolysin, and undergoes enzyme-mediated diassembly upon subtilisin addition. This enzyme-responsive hydrogel provides a semi-rigid, highly hydrated and biocompatible environment that also holds the LC display in place. A dual layer design was developed, where a phospholipid-loaded upper gel layer was separated from the LC display by a phospholipid free lower layer. Subtilisin (0.15 mM) digested both layers to give a gel-to-sol transition after several hours that liberated the phospholipid and produced a light-to-dark optical change in the LC display. The optical response was dependent upon the gel-to-sol transition; elastase or common components of serum did not disassemble the Fmoc-TL-OMe hydrogel and did not give an optical response.

LanguageEnglish
Pages1188-1193
Number of pages6
JournalSoft Matter
Volume9
Issue number4
Early online date26 Nov 2012
DOIs
Publication statusPublished - 2013

Fingerprint

Liquid Crystals
Hydrogels
Hydrogel
Liquid crystal displays
liquid crystals
enzymes
Subtilisin
Phospholipids
Gels
gels
Polymethyl Methacrylate
Enzymes
Thermolysin
enzyme activity
Pancreatic Elastase
Optical sensors
Enzyme activity
optical measuring instruments
serums
peptides

Keywords

  • interfacing biodegradable molecular hydrogels
  • liquid crystals
  • self-assembled Fmoc-peptide hydrogel
  • optical sensor
  • enzyme activity

Cite this

Lin, I-Hsin ; Birchall, Louise S. ; Hodson, Nigel ; Ulijn, Rein V. ; Webb, Simon J. / Interfacing biodegradable molecular hydrogels with liquid crystals. In: Soft Matter. 2013 ; Vol. 9, No. 4. pp. 1188-1193.
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Lin, I-H, Birchall, LS, Hodson, N, Ulijn, RV & Webb, SJ 2013, 'Interfacing biodegradable molecular hydrogels with liquid crystals' Soft Matter, vol. 9, no. 4, pp. 1188-1193. https://doi.org/10.1039/c2sm27160e

Interfacing biodegradable molecular hydrogels with liquid crystals. / Lin, I-Hsin; Birchall, Louise S.; Hodson, Nigel; Ulijn, Rein V.; Webb, Simon J.

In: Soft Matter, Vol. 9, No. 4, 2013, p. 1188-1193.

Research output: Contribution to journalArticle

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