Interactive effects of the electron acceptor sulphate and o-cresol on the methanogenic degradation of hexanoate

Sulisti Holmes, Eric Senior, I.A. Watson-Craik

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A three-stage continuous culture system was used to segregate the component microbial groups of a methanogenic hexanoate-degrading association enriched from anaerobic refuse. The inhibitory effects of o-cresol concentrations (2–20 mM) on the fermentative, acetogenic, sulphate-reducing and methanogenic bacteria were then assessed in the presence of either 1.4 or 3.5 mM sulphate in the influent medium. The sulphate-reducing bacteria (SRB) in the 1.4 mM sulphate-supplemented systems were the most sensitive to o-cresol, with 29.3 and 56.6% inhibition on supplementation with 4 and 6 mM o-cresol, respectively. With 3.5 mM supplementation, inhibition was 4.5 and 19.4%, respectively. Methanogenesis was not inhibited by concentrations <10 mM o-cresol, and complete inhibition was recorded only at concentrations ⩾10 mM. Both fermentation and acetogenesis were affected by inhibition of the electron sinks. The increase in influent sulphate concentration promoted electron flow to sulphidogenesis, as predicted on thermodynamic criteria, but did not affect the relative sensitivity of the different physiological groups.
LanguageEnglish
Pages561-576
Number of pages16
JournalWater Research
Volume36
Issue number3
DOIs
Publication statusPublished - Feb 2002

Fingerprint

sulfate
Degradation
electron
degradation
Electrons
sulfate-reducing bacterium
methanogenic bacterium
Methanogens
methanogenesis
Enzyme inhibition
refuse
fermentation
thermodynamics
Fermentation
Bacteria
effect
Sulfates
Association reactions
Thermodynamics

Keywords

  • o-Cresol
  • Methanogenesis
  • Sulphate-reducing bacteria
  • Inhibition

Cite this

Holmes, Sulisti ; Senior, Eric ; Watson-Craik, I.A. / Interactive effects of the electron acceptor sulphate and o-cresol on the methanogenic degradation of hexanoate. In: Water Research. 2002 ; Vol. 36, No. 3. pp. 561-576.
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abstract = "A three-stage continuous culture system was used to segregate the component microbial groups of a methanogenic hexanoate-degrading association enriched from anaerobic refuse. The inhibitory effects of o-cresol concentrations (2–20 mM) on the fermentative, acetogenic, sulphate-reducing and methanogenic bacteria were then assessed in the presence of either 1.4 or 3.5 mM sulphate in the influent medium. The sulphate-reducing bacteria (SRB) in the 1.4 mM sulphate-supplemented systems were the most sensitive to o-cresol, with 29.3 and 56.6{\%} inhibition on supplementation with 4 and 6 mM o-cresol, respectively. With 3.5 mM supplementation, inhibition was 4.5 and 19.4{\%}, respectively. Methanogenesis was not inhibited by concentrations <10 mM o-cresol, and complete inhibition was recorded only at concentrations ⩾10 mM. Both fermentation and acetogenesis were affected by inhibition of the electron sinks. The increase in influent sulphate concentration promoted electron flow to sulphidogenesis, as predicted on thermodynamic criteria, but did not affect the relative sensitivity of the different physiological groups.",
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Interactive effects of the electron acceptor sulphate and o-cresol on the methanogenic degradation of hexanoate. / Holmes, Sulisti; Senior, Eric; Watson-Craik, I.A.

In: Water Research, Vol. 36, No. 3, 02.2002, p. 561-576.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Senior, Eric

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