Interactions with nanoscale topography: adhesion quantification and signal transduction in cells of osteogenic and multipotent lineage

M.J.P Biggs, R.G. Richards, N. Gadegaard, R.J. McMurray, S. Affrossman, C.D.W. Wilkinson, R.O.C. Oreffo, M.J. Dalby

Research output: Contribution to journalArticle

151 Citations (Scopus)

Abstract

Polymeric medical devices widely used in orthopedic surgery play key roles in fracture fixation and orthopedic implant design. Topographical modification and surface micro-roughness of these devices regulate cellular adhesion, a process fundamental in the initiation of osteoinduction and osteogenesis. Advances in fabrication techniques have evolved the field of surface modification; in particular, nanotedulology has allowed the development of nanoscale substrates for the investigation into cell-nanofeature interactions. In this study human osteoblasts (HOBS) were cultured on ordered nanoscale pits and random nano "craters" and "islands". Adhesion subtypes were quantified by immunofluorescent microscopy and cell-substrate interactions investigated via immuno-scanning electron microscopy. To investigate the effects of these substrates on cellular function 1.7 k microarray analysis was used to establish gene profiles of enriched STRO-1+ progenitor cell populations cultured on these nanotopographies. Nanotopographies affected the formation of adhesions on experimental substrates. Adhesion formation was prominent on planar control substrates and reduced on nanocrater and nanoisland topographies; nanopits, however, were shown to inhibit directly the formation of large adhesions. STRO-1+ progenitor cells cultured on experimental substrates revealed significant changes in genetic expression. This study implicates nanotopographical modification as a significant modulator of osteoblast adhesion and cellular function in mesenchymal populations.
Original languageEnglish
Pages (from-to)195-208
Number of pages13
JournalJournal of Biomedical Materials Research Part A
Volume91A
Issue number1
DOIs
Publication statusPublished - Oct 2009

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Signal transduction
Osteoblasts
Cell Communication
Topography
Orthopedics
Signal Transduction
Stem Cells
Adhesion
Equipment and Supplies
Fracture Fixation
Immunoelectron Microscopy
Substrates
Microarray Analysis
Islands
Osteogenesis
Population
Microscopy
Fracture fixation
Genes
Microarrays

Keywords

  • osteoblasts
  • mesenchymal stem cells
  • focal adhesions
  • genomic regulation
  • nanotopography
  • nanobioscience
  • scanning electron-microscopy
  • poly-l/dl-lactide
  • focal adhesion
  • osteoblast adhesion
  • matrix adhesions
  • dynamics
  • bone
  • mechanotransduction
  • differentiation
  • kinase

Cite this

Biggs, M. J. P., Richards, R. G., Gadegaard, N., McMurray, R. J., Affrossman, S., Wilkinson, C. D. W., ... Dalby, M. J. (2009). Interactions with nanoscale topography: adhesion quantification and signal transduction in cells of osteogenic and multipotent lineage. Journal of Biomedical Materials Research Part A, 91A(1), 195-208. https://doi.org/10.1002/jbm.a.32196
Biggs, M.J.P ; Richards, R.G. ; Gadegaard, N. ; McMurray, R.J. ; Affrossman, S. ; Wilkinson, C.D.W. ; Oreffo, R.O.C. ; Dalby, M.J. / Interactions with nanoscale topography: adhesion quantification and signal transduction in cells of osteogenic and multipotent lineage. In: Journal of Biomedical Materials Research Part A. 2009 ; Vol. 91A, No. 1. pp. 195-208.
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Biggs, MJP, Richards, RG, Gadegaard, N, McMurray, RJ, Affrossman, S, Wilkinson, CDW, Oreffo, ROC & Dalby, MJ 2009, 'Interactions with nanoscale topography: adhesion quantification and signal transduction in cells of osteogenic and multipotent lineage', Journal of Biomedical Materials Research Part A, vol. 91A, no. 1, pp. 195-208. https://doi.org/10.1002/jbm.a.32196

Interactions with nanoscale topography: adhesion quantification and signal transduction in cells of osteogenic and multipotent lineage. / Biggs, M.J.P; Richards, R.G.; Gadegaard, N.; McMurray, R.J.; Affrossman, S.; Wilkinson, C.D.W.; Oreffo, R.O.C.; Dalby, M.J.

In: Journal of Biomedical Materials Research Part A, Vol. 91A, No. 1, 10.2009, p. 195-208.

Research output: Contribution to journalArticle

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AU - Biggs, M.J.P

AU - Richards, R.G.

AU - Gadegaard, N.

AU - McMurray, R.J.

AU - Affrossman, S.

AU - Wilkinson, C.D.W.

AU - Oreffo, R.O.C.

AU - Dalby, M.J.

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KW - scanning electron-microscopy

KW - poly-l/dl-lactide

KW - focal adhesion

KW - osteoblast adhesion

KW - matrix adhesions

KW - dynamics

KW - bone

KW - mechanotransduction

KW - differentiation

KW - kinase

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M3 - Article

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