Interactions of silver nanoparticles with Pseudomonas putida biofilms

Julia Fabrega, Joanna Renshaw, Jamie R. Lead

Research output: Contribution to journalArticle

183 Citations (Scopus)

Abstract

Silver nanoparticles (Ag NPs) may present a risk to the environment due to their expected toxicity and wide exposure. The interactions between Ag NPs and laboratory-grown Pseudomonas putida biofilms were investigated under a range of environmentally relevant conditions (pH 6 and 7.5; presence and absence of Suwannee River fulvic acid (SRFA)) over 4 days. In the absence of SRFA, there was extensive sloughing of the biofilm bacteria into suspension implying NP-bacterial interactions and potential effects on NP transport in the environment. In the presence of SRFA, sloughing of cells into suspension was reduced under some conditions and Ag NPs and their aggregates were observed and quantified on and in the bacterial cells in the biofilm. Viability of the cells in all cases appear unchanged by the presence of Ag NPs. Cell viability was independent of the concentration of NPs in solution, but sloughing rates varied substantially, sometimes in a dose-dependent manner. The results suggest that biofilms are impacted by Ag NPs when SRFA was not present, and that SRFA increases uptake and bioaccumulation of Ag NPs to biofilms, perhaps resulting in longer term effects, which need further investigation. 

LanguageEnglish
Pages9004-9009
Number of pages6
JournalEnvironmental Science and Technology
Volume43
Issue number23
Early online date30 Oct 2009
DOIs
Publication statusPublished - 1 Dec 2009

Fingerprint

Biofilms
fulvic acid
Silver
biofilm
silver
Rivers
Nanoparticles
river
Suspensions
viability
Cells
Bioaccumulation
bioaccumulation
Toxicity
Bacteria
nanoparticle
toxicity
bacterium

Keywords

  • silver nanoparticles
  • pseudomonas putida biofilms
  • interactions
  • Ag NPs
  • environmental risk
  • toxicity
  • Suwannee River fulvic acid
  • SRFA

Cite this

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Interactions of silver nanoparticles with Pseudomonas putida biofilms. / Fabrega, Julia; Renshaw, Joanna; Lead, Jamie R.

In: Environmental Science and Technology, Vol. 43, No. 23, 01.12.2009, p. 9004-9009.

Research output: Contribution to journalArticle

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