Integrated multi-material portable 3D-printed platform for electrochemical detection of dopamine and glucose

Research output: Contribution to journalArticlepeer-review

3 Downloads (Pure)

Abstract

3D-printing has become a fundamental part of research in many areas of investigation since it provides rapid and personalized production of parts that meet very specific user needs. Biosensing is not an exception, and production of electrochemical sensors that can detect a variety of redox mediators and biologically relevant molecules has been widely reported. However, most 3D-printed electrochemical sensors detailed in the literature rely on big, individual, single-material electrodes that require large sample volumes to perform effectively. Our work exploits multi-material fused filament fabrication 3D-printing to produce a compact electrochemical sensor able to operate with only 100 μL of sample. We report cyclic voltammetry, differential pulse voltammetry, and chronoamperometry results to assess sensor performance and sensitivity. We investigated the influence of layer print orientation and layer thickness on the electrochemical performance of the sensor, and used the optimal parameters to produce the final device. The integrated 3D-printed platform successfully detects electrochemical activity for hexaammineruthenium(III) chloride and potassium ferricyanide (0.1 mM to 2 mM in 100 mM KCl), dopamine (50 μM to 1 mM in 1×PBS), and glucose via mediated amperometric glucose oxidase enzyme-based sensing (1 mM to 12 mM in 1×PBS), indicating good acceptance of biological modification. These results reveal the exciting potential of multi-material 3D-printing and how it can be used for the rapid development of efficient, small, integrated, personalized electrochemical biosensors.
Original languageEnglish
Pages (from-to)4598-4606
Number of pages9
JournalAnalyst
Volume147
Issue number20
Early online date16 Sep 2022
DOIs
Publication statusPublished - 10 Oct 2022

Keywords

  • 3D printing
  • electrochemical sensors
  • personalized electrochemical biosensors

Fingerprint

Dive into the research topics of 'Integrated multi-material portable 3D-printed platform for electrochemical detection of dopamine and glucose'. Together they form a unique fingerprint.

Cite this