Integrated modelling of lysozyme ultrafiltration

Vítor Magueijo, Viriato Semiao, Maria Norberta de Pinho

Research output: Contribution to journalArticle

  • 5 Citations

Abstract

Ultrafiltration of a ternary system lysozyme/sodium chloride/water is modelled through the integration of the surface force-pore flow (SFPF) model with the feed flow transport equations and recurring to computational fluid dynamics (CFD). Permeation experiments are performed using an ultrafiltration laboratory cell with a slit feed channel (h = 1.2 mm ≪ l = 200 mm, w = 30 mm) and a laboratory-made cellulose acetate membrane characterized by an hydraulic permeability of 5.9 × 10-11 m s-1 Pa-1 and a molecular weight cut-off of 60 kDa. The operating pressures ranged from 2 to 8 bar and the salt concentrations from 6 × 10-4 to 0.1 M. The ultrafiltration performance is simulated at different operating pressures and salt concentrations through multicomponent mass transfer modelling with incorporation of membrane/protein electrostatic interactions. The predictions of the lysozyme apparent rejection coefficients and permeation fluxes are in very good agreement with the experimental results.

LanguageEnglish
Pages133-143
Number of pages11
JournalJournal of Membrane Science
Volume286
Issue number1-2
DOIs
Publication statusPublished - 15 Dec 2006

Fingerprint

lysozyme
Ultrafiltration
Muramidase
Enzymes
membranes
salts
Permeation
sodium chlorides
Salts
computational fluid dynamics
ternary systems
cellulose
hydraulics
rejection
mass transfer
Membranes
slits
Pressure
acetates
molecular weight

Keywords

  • CFD
  • electrostatic interaction
  • modelling
  • multicomponent diffusion
  • protein ultrafiltration

Cite this

Magueijo, Vítor ; Semiao, Viriato ; de Pinho, Maria Norberta. / Integrated modelling of lysozyme ultrafiltration. In: Journal of Membrane Science. 2006 ; Vol. 286, No. 1-2. pp. 133-143.
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Integrated modelling of lysozyme ultrafiltration. / Magueijo, Vítor; Semiao, Viriato; de Pinho, Maria Norberta.

In: Journal of Membrane Science, Vol. 286, No. 1-2, 15.12.2006, p. 133-143.

Research output: Contribution to journalArticle

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AU - Semiao, Viriato

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