Insulin aggregation tracked by its intrinsic TRES

Li Hung C. Chung, David J. S. Birch, Vladislav Vyshemirsky, Maxim G. Ryadnov, Olaf J. Rolinski

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Abstract

Time-resolved emission spectra (TRES) have been used to detect conformational changes of intrinsic tyrosines within bovine insulin at a physiological pH. The approach offers the ability to detect the initial stages of insulin aggregation at the molecular level. The data analysis has revealed the existence of at least three fluorescent species undergoing dielectric relaxation and significant spectral changes due to insulin aggregation. The results indicate suitability of the intrinsic TRES approach for insulin studies and for monitoring its stability during storage and aggregation in insulin delivery devices.
Original languageEnglish
Number of pages5
JournalApplied Physics Letters
Volume111
Early online date27 Dec 2017
DOIs
Publication statusE-pub ahead of print - 27 Dec 2017

Keywords

  • biomolecules
  • conformational dynamics
  • dielectrics
  • emission spectra
  • biological physics

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Olaf Rolinski

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