Insulin aggregation tracked by its intrinsic TRES

Li Hung C. Chung; David J. S. Birch; Vladislav Vyshemirsky; Maxim G. Ryadnov; Olaf J. Rolinski

doi:10.1063/1.5008477

Insulin aggregation tracked by its intrinsic TRES

Li Hung C. Chung, David J. S. Birch, Vladislav Vyshemirsky, Maxim G. Ryadnov, Olaf J. Rolinski

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Abstract

Time-resolved emission spectra (TRES) have been used to detect conformational changes of intrinsic tyrosines within bovine insulin at a physiological pH. The approach offers the ability to detect the initial stages of insulin aggregation at the molecular level. The data analysis has revealed the existence of at least three fluorescent species undergoing dielectric relaxation and significant spectral changes due to insulin aggregation. The results indicate suitability of the intrinsic TRES approach for insulin studies and for monitoring its stability during storage and aggregation in insulin delivery devices.

Original language	English
Number of pages	5
Journal	Applied Physics Letters
Volume	111
Early online date	27 Dec 2017
DOIs	https://doi.org/10.1063/1.5008477
Publication status	E-pub ahead of print - 27 Dec 2017

Keywords

biomolecules
conformational dynamics
dielectrics
emission spectra
biological physics

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10.1063/1.5008477

Chung-etal-APL2017-Insulin-aggregation-tracked-by-its-intrinsic-TRESAccepted author manuscript, 892 KB

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title = "Insulin aggregation tracked by its intrinsic TRES",

abstract = "Time-resolved emission spectra (TRES) have been used to detect conformational changes of intrinsic tyrosines within bovine insulin at a physiological pH. The approach offers the ability to detect the initial stages of insulin aggregation at the molecular level. The data analysis has revealed the existence of at least three fluorescent species undergoing dielectric relaxation and significant spectral changes due to insulin aggregation. The results indicate suitability of the intrinsic TRES approach for insulin studies and for monitoring its stability during storage and aggregation in insulin delivery devices.",

keywords = "biomolecules, conformational dynamics, dielectrics, emission spectra, biological physics",

author = "Chung, {Li Hung C.} and Birch, {David J. S.} and Vladislav Vyshemirsky and Ryadnov, {Maxim G.} and Rolinski, {Olaf J.}",

note = "This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. The following article appeared in Chung, LHC, Birch, DJS, Vyshemirsky, V, Ryadnov, MG & Rolinski, OJ 2017, 'Insulin aggregation tracked by its intrinsic TRES' Applied Physics Letters, vol 111 and may be found at https://doi.org/10.1063/1.5008477.",

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T1 - Insulin aggregation tracked by its intrinsic TRES

AU - Chung, Li Hung C.

AU - Birch, David J. S.

AU - Vyshemirsky, Vladislav

AU - Ryadnov, Maxim G.

AU - Rolinski, Olaf J.

N1 - This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. The following article appeared in Chung, LHC, Birch, DJS, Vyshemirsky, V, Ryadnov, MG & Rolinski, OJ 2017, 'Insulin aggregation tracked by its intrinsic TRES' Applied Physics Letters, vol 111 and may be found at https://doi.org/10.1063/1.5008477.

PY - 2017/12/27

Y1 - 2017/12/27

N2 - Time-resolved emission spectra (TRES) have been used to detect conformational changes of intrinsic tyrosines within bovine insulin at a physiological pH. The approach offers the ability to detect the initial stages of insulin aggregation at the molecular level. The data analysis has revealed the existence of at least three fluorescent species undergoing dielectric relaxation and significant spectral changes due to insulin aggregation. The results indicate suitability of the intrinsic TRES approach for insulin studies and for monitoring its stability during storage and aggregation in insulin delivery devices.

AB - Time-resolved emission spectra (TRES) have been used to detect conformational changes of intrinsic tyrosines within bovine insulin at a physiological pH. The approach offers the ability to detect the initial stages of insulin aggregation at the molecular level. The data analysis has revealed the existence of at least three fluorescent species undergoing dielectric relaxation and significant spectral changes due to insulin aggregation. The results indicate suitability of the intrinsic TRES approach for insulin studies and for monitoring its stability during storage and aggregation in insulin delivery devices.

KW - biomolecules

KW - conformational dynamics

KW - dielectrics

KW - emission spectra

KW - biological physics

UR - http://aip.scitation.org/journal/apl

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DO - 10.1063/1.5008477

M3 - Article

SN - 0003-6951

VL - 111

JO - Applied Physics Letters

JF - Applied Physics Letters

ER -

Insulin aggregation tracked by its intrinsic TRES

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