Instrumentation for fluorescence lifetime measurement using photon counting

David J. S. Birch, Graham Hungerford, David McLoskey, Kulwinder Sagoo, Philip Yip

Research output: Chapter in Book/Report/Conference proceedingChapter

29 Downloads (Pure)

Abstract

We describe the evolution of HORIBA Jobin Yvon IBH Ltd, and its time-correlated single-photon counting (TCSPC) products, from university research beginnings through to its present place as a market leader in fluorescence lifetime spectroscopy. The company philosophy is to ensure leading-edge research capabilities continue to be incorporated into instruments in order to meet the needs of the diverse range of customer applications, which span a multitude of scientific and engineering disciplines. We illustrate some of the range of activities of a scientific instrument company in meeting this goal and highlight by way of an exemplar the performance of the versatile DeltaFlex instrument in measuring fluorescence lifetimes. This includes resolving fluorescence lifetimes down to 5 ps, as frequently observed in energy transfer, nanoparticle metrology with sub-nanometre resolution and measuring a fluorescence lifetime in as little as 60 μs for the study of transient species and kinetics.
Original languageEnglish
Title of host publicationFluorescence in Industry
EditorsBruno Pedras
Place of PublicationSwitzerland
PublisherSpringer
Chapter3
Pages103-133
Number of pages31
Volume18
ISBN (Electronic)978-3-030-20033-6
ISBN (Print)978-3-030-20032-9
DOIs
Publication statusPublished - 2 Jul 2019

Publication series

NameSpringer Series on Fluorescence
PublisherSpringer
Volume18

Keywords

  • DeltaFlex
  • fluorescence lifetime
  • HORIBA Jobin Yvon IBH
  • photon counting
  • TCSPC

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  • Projects

    Research Output

    TCSPC camera for real time video rate FLIM acquisition based on CMOS technology

    Hungerford, G., McLoskey, D., Hirsch, R., Yip, P., Birch, D., Johnston, N. & Henderson, R., 7 Feb 2020, In : Biophysical Journal. 118, 3, Supplement 1, p. 308a-308a 1 p.

    Research output: Contribution to journalConference Contribution

  • 12th FluoroFest International Workshop April 24–26, 2017

    Birch, D. J. S., 29 Jan 2018, In : Methods and Applications in Fluorescence . 6, 1, 2 p., 020201.

    Research output: Contribution to journalEditorial

    Open Access
  • 1 Citation (Scopus)

    A lifetime in fluorescence: its measurement and application

    Birch, D., 24 Apr 2017.

    Research output: Contribution to conferenceKeynote

  • Prizes

    Horiba Lifetime Achievement Award

    David Birch (Recipient), 26 Apr 2017

    Prize: Prize (including medals and awards)

  • Cite this

    Birch, D. J. S., Hungerford, G., McLoskey, D., Sagoo, K., & Yip, P. (2019). Instrumentation for fluorescence lifetime measurement using photon counting. In B. Pedras (Ed.), Fluorescence in Industry (Vol. 18, pp. 103-133). (Springer Series on Fluorescence; Vol. 18). Switzerland: Springer. https://doi.org/10.1007/4243_2018_2