Insight into potential probiotic markers predicted in Lactobacillus pentosus MP-10 genome sequence

Hikmate Abriouel, Beatriz Pérez Montoro, Carlos S. Casimiro-Soriguer, Antonio J. Perez-Pulido, Charles W. Knapp, Natasha C. Gomez, Sonia Castillo-Gutierrez, Maria Dolores Estudillo-Martinez, Antonio Gálvez, Nabil Benomar

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Lactobacillus pentosus MP-10 is a potential probiotic lactic acid bacterium (LAB) originally isolated from naturally fermented Aloreña green table olives. The entire genome sequence was annotated to in-silico analyze the molecular mechanisms involved in the adaptation of L. pentosus MP-10 to the human gastrointestinal tract (GIT), such as carbohydrate metabolism (related with prebiotic utilization) and the proteins involved in bacteria-host interactions. We predicted an arsenal of genes coding for carbohydrate-modifying enzymes to modify oligo- and polysaccharides, such as glycoside hydrolases, glycoside transferases and isomerases, and other enzymes involved in complex carbohydrate metabolism especially starch, raffinose and levan. These enzymes represent key indicators of the bacteria’s adaptation to the GIT environment, since they involve the metabolism and assimilation of complex carbohydrates not digested by human enzymes. We also detected key probiotic ligands (surface proteins, excreted or secreted proteins) involved in the adhesion to host cells such as adhesion to mucus, epithelial cells or extracellular matrix, and plasma components; also, moonlighting proteins or multifunctional proteins were found that could be involved in adhesion to epithelial cells and/or extracellular matrix proteins and also affect host immunomodulation. In-silico analysis of the genome sequence of L. pentosus MP-10 is an important initial step to screen for genes encoding for proteins that may provide probiotic features, and thus provides one new routes for screening and studying this potentially probiotic bacterium.
LanguageEnglish
Article number891
Number of pages17
JournalFrontiers in Microbiology
Volume8
DOIs
Publication statusPublished - 22 May 2017

Fingerprint

Probiotics
Genome
Bacteria
Carbohydrate Metabolism
Enzymes
Proteins
Computer Simulation
Gastrointestinal Tract
Epithelial Cells
Carbohydrates
Raffinose
Prebiotics
Isomerases
Glycosyltransferases
Immunomodulation
Extracellular Matrix Proteins
Glycoside Hydrolases
Olea
Mucus
Oligosaccharides

Keywords

  • aloreña table olives
  • lactobacillus pentosus
  • probiotics
  • in silico analysis
  • carbohydrate metabolism
  • host interaction

Cite this

Abriouel, H., Pérez Montoro, B., Casimiro-Soriguer, C. S., Perez-Pulido, A. J., Knapp, C. W., Gomez, N. C., ... Benomar, N. (2017). Insight into potential probiotic markers predicted in Lactobacillus pentosus MP-10 genome sequence. Frontiers in Microbiology, 8, [891]. https://doi.org/10.3389/fmicb.2017.00891
Abriouel, Hikmate ; Pérez Montoro, Beatriz ; Casimiro-Soriguer, Carlos S. ; Perez-Pulido, Antonio J. ; Knapp, Charles W. ; Gomez, Natasha C. ; Castillo-Gutierrez, Sonia ; Estudillo-Martinez, Maria Dolores ; Gálvez, Antonio ; Benomar, Nabil. / Insight into potential probiotic markers predicted in Lactobacillus pentosus MP-10 genome sequence. In: Frontiers in Microbiology. 2017 ; Vol. 8.
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Abriouel, H, Pérez Montoro, B, Casimiro-Soriguer, CS, Perez-Pulido, AJ, Knapp, CW, Gomez, NC, Castillo-Gutierrez, S, Estudillo-Martinez, MD, Gálvez, A & Benomar, N 2017, 'Insight into potential probiotic markers predicted in Lactobacillus pentosus MP-10 genome sequence' Frontiers in Microbiology, vol. 8, 891. https://doi.org/10.3389/fmicb.2017.00891

Insight into potential probiotic markers predicted in Lactobacillus pentosus MP-10 genome sequence. / Abriouel, Hikmate; Pérez Montoro, Beatriz; Casimiro-Soriguer, Carlos S.; Perez-Pulido, Antonio J.; Knapp, Charles W.; Gomez, Natasha C.; Castillo-Gutierrez, Sonia; Estudillo-Martinez, Maria Dolores; Gálvez, Antonio; Benomar, Nabil.

In: Frontiers in Microbiology, Vol. 8, 891, 22.05.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Insight into potential probiotic markers predicted in Lactobacillus pentosus MP-10 genome sequence

AU - Abriouel, Hikmate

AU - Pérez Montoro, Beatriz

AU - Casimiro-Soriguer, Carlos S.

AU - Perez-Pulido, Antonio J.

AU - Knapp, Charles W.

AU - Gomez, Natasha C.

AU - Castillo-Gutierrez, Sonia

AU - Estudillo-Martinez, Maria Dolores

AU - Gálvez, Antonio

AU - Benomar, Nabil

PY - 2017/5/22

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N2 - Lactobacillus pentosus MP-10 is a potential probiotic lactic acid bacterium (LAB) originally isolated from naturally fermented Aloreña green table olives. The entire genome sequence was annotated to in-silico analyze the molecular mechanisms involved in the adaptation of L. pentosus MP-10 to the human gastrointestinal tract (GIT), such as carbohydrate metabolism (related with prebiotic utilization) and the proteins involved in bacteria-host interactions. We predicted an arsenal of genes coding for carbohydrate-modifying enzymes to modify oligo- and polysaccharides, such as glycoside hydrolases, glycoside transferases and isomerases, and other enzymes involved in complex carbohydrate metabolism especially starch, raffinose and levan. These enzymes represent key indicators of the bacteria’s adaptation to the GIT environment, since they involve the metabolism and assimilation of complex carbohydrates not digested by human enzymes. We also detected key probiotic ligands (surface proteins, excreted or secreted proteins) involved in the adhesion to host cells such as adhesion to mucus, epithelial cells or extracellular matrix, and plasma components; also, moonlighting proteins or multifunctional proteins were found that could be involved in adhesion to epithelial cells and/or extracellular matrix proteins and also affect host immunomodulation. In-silico analysis of the genome sequence of L. pentosus MP-10 is an important initial step to screen for genes encoding for proteins that may provide probiotic features, and thus provides one new routes for screening and studying this potentially probiotic bacterium.

AB - Lactobacillus pentosus MP-10 is a potential probiotic lactic acid bacterium (LAB) originally isolated from naturally fermented Aloreña green table olives. The entire genome sequence was annotated to in-silico analyze the molecular mechanisms involved in the adaptation of L. pentosus MP-10 to the human gastrointestinal tract (GIT), such as carbohydrate metabolism (related with prebiotic utilization) and the proteins involved in bacteria-host interactions. We predicted an arsenal of genes coding for carbohydrate-modifying enzymes to modify oligo- and polysaccharides, such as glycoside hydrolases, glycoside transferases and isomerases, and other enzymes involved in complex carbohydrate metabolism especially starch, raffinose and levan. These enzymes represent key indicators of the bacteria’s adaptation to the GIT environment, since they involve the metabolism and assimilation of complex carbohydrates not digested by human enzymes. We also detected key probiotic ligands (surface proteins, excreted or secreted proteins) involved in the adhesion to host cells such as adhesion to mucus, epithelial cells or extracellular matrix, and plasma components; also, moonlighting proteins or multifunctional proteins were found that could be involved in adhesion to epithelial cells and/or extracellular matrix proteins and also affect host immunomodulation. In-silico analysis of the genome sequence of L. pentosus MP-10 is an important initial step to screen for genes encoding for proteins that may provide probiotic features, and thus provides one new routes for screening and studying this potentially probiotic bacterium.

KW - aloreña table olives

KW - lactobacillus pentosus

KW - probiotics

KW - in silico analysis

KW - carbohydrate metabolism

KW - host interaction

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DO - 10.3389/fmicb.2017.00891

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JO - Frontiers in Microbiology

T2 - Frontiers in Microbiology

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