Abstract
Streptomyces rimosus has greatly influenced human history as a producer of many important secondary polyketide metabolites, such as oxytetracycline (OTC). The traditional screen and mutation program has resulted in a dramatic increase in OTC production. The availability of multiple semi-complete genome sequences of S. rimosus facilitates attempts to systematically address basic questions in genome evolution. We refer to such efforts as micro-evolutionary analysis. We report the results of comparative analysis of semi-complete genome sequences of three S. rimosus strains from the genealogy map (G7, M4018 and 23383) with different OTC production levels using genome comparison (single nucleotide polymorphisms, SNPs) method. These data were used to assess the influence of microevolution on the physiology, genetics and evolution of S. rimosus. Some SNPs were found in primary metabolism related genes which might affect the final OTC production. We further discussed the microevolution of primary metabolite genes (Glucose-6-phosphate dehydrogenase, zwf) and regulatory genes (redox regulator, rex) in S. rimosus. Using SOLEXA sequencing data, the phylogenetic trees of zwf and rex were constructed. The results indicate that S. rimosus is closely related with Streptomyces albus and represents a distinct evolutionary lineage compared with other Streptomyces. Our research cannot only provide important information for genotyping and evolutionary research of S. rimosus, but can also make possible the development of an informed view of genotype and phenotype.
| Language | English |
|---|---|
| Pages | 5229-5236 |
| Number of pages | 8 |
| Journal | African Journal of Microbiology Research |
| Volume | 6 |
| Issue number | 24 |
| DOIs | |
| Publication status | Published - 28 Jun 2012 |
Fingerprint
Keywords
- microevolution
- streptomyces rimosus
- zwf
- rex
- genes
- single nucleotide polymorphisms (SNPs)
- redox sensor
- glucose-6-phosphate dehydrogenase
Cite this
}
Insight into microevolution of Streptomyces rimosus based on analysis of zwf and rex genes. / Tang, Zhenyu; Herron, Paul R.; Hunter, Iain S.; Zhang, Siliang; Guo, Meijin.
In: African Journal of Microbiology Research, Vol. 6, No. 24, 28.06.2012, p. 5229-5236.Research output: Contribution to journal › Article
TY - JOUR
T1 - Insight into microevolution of Streptomyces rimosus based on analysis of zwf and rex genes
AU - Tang, Zhenyu
AU - Herron, Paul R.
AU - Hunter, Iain S.
AU - Zhang, Siliang
AU - Guo, Meijin
PY - 2012/6/28
Y1 - 2012/6/28
N2 - Streptomyces rimosus has greatly influenced human history as a producer of many important secondary polyketide metabolites, such as oxytetracycline (OTC). The traditional screen and mutation program has resulted in a dramatic increase in OTC production. The availability of multiple semi-complete genome sequences of S. rimosus facilitates attempts to systematically address basic questions in genome evolution. We refer to such efforts as micro-evolutionary analysis. We report the results of comparative analysis of semi-complete genome sequences of three S. rimosus strains from the genealogy map (G7, M4018 and 23383) with different OTC production levels using genome comparison (single nucleotide polymorphisms, SNPs) method. These data were used to assess the influence of microevolution on the physiology, genetics and evolution of S. rimosus. Some SNPs were found in primary metabolism related genes which might affect the final OTC production. We further discussed the microevolution of primary metabolite genes (Glucose-6-phosphate dehydrogenase, zwf) and regulatory genes (redox regulator, rex) in S. rimosus. Using SOLEXA sequencing data, the phylogenetic trees of zwf and rex were constructed. The results indicate that S. rimosus is closely related with Streptomyces albus and represents a distinct evolutionary lineage compared with other Streptomyces. Our research cannot only provide important information for genotyping and evolutionary research of S. rimosus, but can also make possible the development of an informed view of genotype and phenotype.
AB - Streptomyces rimosus has greatly influenced human history as a producer of many important secondary polyketide metabolites, such as oxytetracycline (OTC). The traditional screen and mutation program has resulted in a dramatic increase in OTC production. The availability of multiple semi-complete genome sequences of S. rimosus facilitates attempts to systematically address basic questions in genome evolution. We refer to such efforts as micro-evolutionary analysis. We report the results of comparative analysis of semi-complete genome sequences of three S. rimosus strains from the genealogy map (G7, M4018 and 23383) with different OTC production levels using genome comparison (single nucleotide polymorphisms, SNPs) method. These data were used to assess the influence of microevolution on the physiology, genetics and evolution of S. rimosus. Some SNPs were found in primary metabolism related genes which might affect the final OTC production. We further discussed the microevolution of primary metabolite genes (Glucose-6-phosphate dehydrogenase, zwf) and regulatory genes (redox regulator, rex) in S. rimosus. Using SOLEXA sequencing data, the phylogenetic trees of zwf and rex were constructed. The results indicate that S. rimosus is closely related with Streptomyces albus and represents a distinct evolutionary lineage compared with other Streptomyces. Our research cannot only provide important information for genotyping and evolutionary research of S. rimosus, but can also make possible the development of an informed view of genotype and phenotype.
KW - microevolution
KW - streptomyces rimosus
KW - zwf
KW - rex
KW - genes
KW - single nucleotide polymorphisms (SNPs)
KW - redox sensor
KW - glucose-6-phosphate dehydrogenase
UR - http://www.academicjournals.org/ajmr/abstracts/abstracts/abstracts2012/28June/Tang%20et%20al.htm
U2 - 10.5897/AJMR12.545
DO - 10.5897/AJMR12.545
M3 - Article
VL - 6
SP - 5229
EP - 5236
JO - African Journal of Microbiology Research
T2 - African Journal of Microbiology Research
JF - African Journal of Microbiology Research
SN - 1996-0808
IS - 24
ER -