Initial stage of cheese production: a molecular modeling study of bovine and camel chymosin complexed with peptides from the chymosin-sensitive region of kappa-casein

J. Sørensen, D. S. Palmer, K. B. Qvist, B. Schiøtt

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Bovine chymosin has long been the preferred enzyme used to coagulate cow's milk, in the initial stage of cheese production, during which it cleaves a specific bond in the milk protein kappa-casein. Recently, camel chymosin has been shown to have a 70% higher clotting activity toward cow's milk and, moreover, to cleave kappa-casein more selectively. Bovine chymosin, on the other hand, is a poor clotting agent toward camel's milk. This paper reports a molecular modeling study aimed at understanding this disparity, based on homology modeling and molecular dynamics simulations using peptide fragments of kappa-casein from cow and camel in both bovine and camel chymosin. The results show that the complex between bovine chymosin and the fragment of camel kappa-casein is indeed less stable in the binding pocket. The results also indicate that this in part may be due to charge repulsion between a lysine residue in bovine chymosin and an arginine residue in the P4 position of camel kappa-casein.
Original languageEnglish
Pages (from-to)5636-5647
Number of pages12
JournalJournal of Agricultural and Food Chemistry
Volume59
Issue number10
DOIs
Publication statusPublished - 1 May 2011

Keywords

  • bovine chymosin
  • peptides
  • cheese production

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  • Research Output

    • 19 Citations
    • 2 Article

    Hot-spot mapping of the interactions between chymosin and bovine κ-casein

    Sørensen, J., Palmer, D. & Schiøtt, B., 8 Jul 2013, In : Journal of Agricultural and Food Chemistry. 61, 33, p. 7949-7959 11 p.

    Research output: Contribution to journalArticle

  • 9 Citations (Scopus)

    Solvent binding analysis and computational alanine scanning of the bovine chymosin-bovine κ-casein complex using molecular integral equation theory

    Palmer, D., Sørensen, J. G. J. G., Schiøtt, B. B. & Fedorov, M. V. M. V., 10 Dec 2013, In : Journal of Chemical Theory and Computation . 9, 12, p. 5706-5717 12 p.

    Research output: Contribution to journalArticle

  • 9 Citations (Scopus)

    Activities

    • 1 Participation in conference

    Tools and Strategies to Find Chemical Probes for Your Protein - The Role of Computer-Aided Drug Discovery

    David Palmer (Participant)

    15 Nov 2013

    Activity: Participating in or organising an event typesParticipation in conference

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