Inhibition of lipopolysaccharide-induced macrophage IL-12 production by Leishmania mexicana amastigotes: the role of cysteine peptidases and the NF-kappaB signaling pathway

P. Cameron, H.A. McGachy, M. Anderson, A. Paul, G.H. Coombs, J.C. Mottram, J. Alexander, R.J. Plevin

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Abstract

Infection with lesion-derived Leishmania mexicana amastigotes inhibited LPS-induced IL-12 production by mouse bone marrow-derived macrophages. This effect was associated with expression of cysteine peptidase B (CPB) because amastigotes of CPB deletion mutants had limited ability to inhibit IL-12 production, whereas preincubation of cells with a CPB inhibitor, cathepsin inhibitor IV, was able to suppress the effect of wild-type amastigotes. Infection with wild-type amastigotes resulted in a time-dependent proteolytic degradation of IB and IB and the related protein NF-B. This effect did not occur with amastigotes of CPB deletion mutants or wild-type promastigotes, which do not express detectable CPB. NF-B DNA binding was also inhibited by amastigote infection, although nuclear translocation of cleaved fragments of p65 NF-B was still observed. Cysteine peptidase inhibitors prevented IB, IB, and NF-B degradation induced by amastigotes, and recombinant CPB2.8, an amastigote-specific isoenzyme of CPB, was shown to degrade GST-IB in vitro. LPS-mediated IB and IB degradation was not affected by these inhibitors, confirming that the site of degradation of IB, IB, and NF-B by the amastigotes was not receptor-driven, proteosomal-mediated cleavage. Infection of bone marrow macrophages with amastigotes resulted in cleavage of JNK and ERK, but not p38 MAPK, whereas preincubation with a cysteine peptidase inhibitor prevented degradation of these proteins, but did not result in enhanced protein kinase activation. Collectively, our results suggest that the amastigote-specific cysteine peptidases of L. mexicana are central to the ability of the parasite to modulate signaling via NF-B and consequently inhibit IL-12 production.
LanguageEnglish
Pages3297-3304
Number of pages7
JournalJournal of Immunology
Volume173
Issue number5
Publication statusPublished - Sep 2004

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Leishmania mexicana
tripeptide aminopeptidase
NF-kappa B
Interleukin-12
Cysteine
Lipopolysaccharides
Peptide Hydrolases
Macrophages
Protease Inhibitors
Infection
Cathepsins
p38 Mitogen-Activated Protein Kinases
Protein Kinases
Isoenzymes
Proteolysis
Parasites
Bone Marrow

Keywords

  • Leishmania mexicana Amastigotes
  • cysteine peptidases
  • LPS-induced IL-12 production
  • NF-kB

Cite this

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title = "Inhibition of lipopolysaccharide-induced macrophage IL-12 production by Leishmania mexicana amastigotes: the role of cysteine peptidases and the NF-kappaB signaling pathway",
abstract = "Infection with lesion-derived Leishmania mexicana amastigotes inhibited LPS-induced IL-12 production by mouse bone marrow-derived macrophages. This effect was associated with expression of cysteine peptidase B (CPB) because amastigotes of CPB deletion mutants had limited ability to inhibit IL-12 production, whereas preincubation of cells with a CPB inhibitor, cathepsin inhibitor IV, was able to suppress the effect of wild-type amastigotes. Infection with wild-type amastigotes resulted in a time-dependent proteolytic degradation of IB and IB and the related protein NF-B. This effect did not occur with amastigotes of CPB deletion mutants or wild-type promastigotes, which do not express detectable CPB. NF-B DNA binding was also inhibited by amastigote infection, although nuclear translocation of cleaved fragments of p65 NF-B was still observed. Cysteine peptidase inhibitors prevented IB, IB, and NF-B degradation induced by amastigotes, and recombinant CPB2.8, an amastigote-specific isoenzyme of CPB, was shown to degrade GST-IB in vitro. LPS-mediated IB and IB degradation was not affected by these inhibitors, confirming that the site of degradation of IB, IB, and NF-B by the amastigotes was not receptor-driven, proteosomal-mediated cleavage. Infection of bone marrow macrophages with amastigotes resulted in cleavage of JNK and ERK, but not p38 MAPK, whereas preincubation with a cysteine peptidase inhibitor prevented degradation of these proteins, but did not result in enhanced protein kinase activation. Collectively, our results suggest that the amastigote-specific cysteine peptidases of L. mexicana are central to the ability of the parasite to modulate signaling via NF-B and consequently inhibit IL-12 production.",
keywords = "Leishmania mexicana Amastigotes, cysteine peptidases, LPS-induced IL-12 production, NF-kB",
author = "P. Cameron and H.A. McGachy and M. Anderson and A. Paul and G.H. Coombs and J.C. Mottram and J. Alexander and R.J. Plevin",
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T1 - Inhibition of lipopolysaccharide-induced macrophage IL-12 production by Leishmania mexicana amastigotes: the role of cysteine peptidases and the NF-kappaB signaling pathway

AU - Cameron, P.

AU - McGachy, H.A.

AU - Anderson, M.

AU - Paul, A.

AU - Coombs, G.H.

AU - Mottram, J.C.

AU - Alexander, J.

AU - Plevin, R.J.

PY - 2004/9

Y1 - 2004/9

N2 - Infection with lesion-derived Leishmania mexicana amastigotes inhibited LPS-induced IL-12 production by mouse bone marrow-derived macrophages. This effect was associated with expression of cysteine peptidase B (CPB) because amastigotes of CPB deletion mutants had limited ability to inhibit IL-12 production, whereas preincubation of cells with a CPB inhibitor, cathepsin inhibitor IV, was able to suppress the effect of wild-type amastigotes. Infection with wild-type amastigotes resulted in a time-dependent proteolytic degradation of IB and IB and the related protein NF-B. This effect did not occur with amastigotes of CPB deletion mutants or wild-type promastigotes, which do not express detectable CPB. NF-B DNA binding was also inhibited by amastigote infection, although nuclear translocation of cleaved fragments of p65 NF-B was still observed. Cysteine peptidase inhibitors prevented IB, IB, and NF-B degradation induced by amastigotes, and recombinant CPB2.8, an amastigote-specific isoenzyme of CPB, was shown to degrade GST-IB in vitro. LPS-mediated IB and IB degradation was not affected by these inhibitors, confirming that the site of degradation of IB, IB, and NF-B by the amastigotes was not receptor-driven, proteosomal-mediated cleavage. Infection of bone marrow macrophages with amastigotes resulted in cleavage of JNK and ERK, but not p38 MAPK, whereas preincubation with a cysteine peptidase inhibitor prevented degradation of these proteins, but did not result in enhanced protein kinase activation. Collectively, our results suggest that the amastigote-specific cysteine peptidases of L. mexicana are central to the ability of the parasite to modulate signaling via NF-B and consequently inhibit IL-12 production.

AB - Infection with lesion-derived Leishmania mexicana amastigotes inhibited LPS-induced IL-12 production by mouse bone marrow-derived macrophages. This effect was associated with expression of cysteine peptidase B (CPB) because amastigotes of CPB deletion mutants had limited ability to inhibit IL-12 production, whereas preincubation of cells with a CPB inhibitor, cathepsin inhibitor IV, was able to suppress the effect of wild-type amastigotes. Infection with wild-type amastigotes resulted in a time-dependent proteolytic degradation of IB and IB and the related protein NF-B. This effect did not occur with amastigotes of CPB deletion mutants or wild-type promastigotes, which do not express detectable CPB. NF-B DNA binding was also inhibited by amastigote infection, although nuclear translocation of cleaved fragments of p65 NF-B was still observed. Cysteine peptidase inhibitors prevented IB, IB, and NF-B degradation induced by amastigotes, and recombinant CPB2.8, an amastigote-specific isoenzyme of CPB, was shown to degrade GST-IB in vitro. LPS-mediated IB and IB degradation was not affected by these inhibitors, confirming that the site of degradation of IB, IB, and NF-B by the amastigotes was not receptor-driven, proteosomal-mediated cleavage. Infection of bone marrow macrophages with amastigotes resulted in cleavage of JNK and ERK, but not p38 MAPK, whereas preincubation with a cysteine peptidase inhibitor prevented degradation of these proteins, but did not result in enhanced protein kinase activation. Collectively, our results suggest that the amastigote-specific cysteine peptidases of L. mexicana are central to the ability of the parasite to modulate signaling via NF-B and consequently inhibit IL-12 production.

KW - Leishmania mexicana Amastigotes

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KW - LPS-induced IL-12 production

KW - NF-kB

UR - http://www.jimmunol.org/cgi/content/abstract/173/5/3297

M3 - Article

VL - 173

SP - 3297

EP - 3304

JO - Journal of Immunology

T2 - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

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