Inhibition of glutathione reductase by chromium (VI)

S. Bibi, F. Angeli, M.H. Grant

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    Chromium (VI) compounds have serious toxic and carcinogenic effects in humans. To exert toxicity and carcinogenicity Cr (VI) must be reduced inside cells, and it can be reduced both enzymatically and non-enyzmatically. Glutathione reductase (GR) has been implicated in the intracellular reduction of Cr (VI). This enzyme normally re-cycles reduced glutathione (GSH) from oxidized glutathione (GSSG), and is essential for protecting cells against intermediates which deplete GSH, and against oxidative stress. During its reduction inside cells Cr (VI) disrupts redox balance, and generates reactive oxygen species. These oxidize GSH and deplete reduced thiols, leading to toxicity and carcinogenesis. Susceptibility to Cr (VI) will depend on the ability of cells to protect themselves by recycling GSH via GR. The effect of exposure to Cr (VI) in vitro on the activity of GR in cells derived from liver (Hep G2 cells), colon (HT 115 cells), larynx (Hep 2 cells) and macrophages (J774.1 cells) was investigated.
    LanguageEnglish
    Title of host publicationProceedings of the British Pharmacological Society Conference 2004
    Publication statusPublished - 2004

    Fingerprint

    Glutathione Reductase
    Glutathione Disulfide
    Chromium Compounds
    chromium hexavalent ion
    Poisons
    Hep G2 Cells
    Recycling
    Larynx
    Sulfhydryl Compounds
    Oxidation-Reduction
    Glutathione
    Reactive Oxygen Species
    Colon
    Carcinogenesis
    Oxidative Stress
    Macrophages
    Liver
    Enzymes

    Keywords

    • bioengineering
    • glutathione reductase
    • chromium
    • pharmacology

    Cite this

    Bibi, S., Angeli, F., & Grant, M. H. (2004). Inhibition of glutathione reductase by chromium (VI). In Proceedings of the British Pharmacological Society Conference 2004
    Bibi, S. ; Angeli, F. ; Grant, M.H. / Inhibition of glutathione reductase by chromium (VI). Proceedings of the British Pharmacological Society Conference 2004. 2004.
    @inbook{9e34cb6893c747859fd2ee5485e3a4ce,
    title = "Inhibition of glutathione reductase by chromium (VI)",
    abstract = "Chromium (VI) compounds have serious toxic and carcinogenic effects in humans. To exert toxicity and carcinogenicity Cr (VI) must be reduced inside cells, and it can be reduced both enzymatically and non-enyzmatically. Glutathione reductase (GR) has been implicated in the intracellular reduction of Cr (VI). This enzyme normally re-cycles reduced glutathione (GSH) from oxidized glutathione (GSSG), and is essential for protecting cells against intermediates which deplete GSH, and against oxidative stress. During its reduction inside cells Cr (VI) disrupts redox balance, and generates reactive oxygen species. These oxidize GSH and deplete reduced thiols, leading to toxicity and carcinogenesis. Susceptibility to Cr (VI) will depend on the ability of cells to protect themselves by recycling GSH via GR. The effect of exposure to Cr (VI) in vitro on the activity of GR in cells derived from liver (Hep G2 cells), colon (HT 115 cells), larynx (Hep 2 cells) and macrophages (J774.1 cells) was investigated.",
    keywords = "bioengineering, glutathione reductase, chromium, pharmacology",
    author = "S. Bibi and F. Angeli and M.H. Grant",
    year = "2004",
    language = "English",
    booktitle = "Proceedings of the British Pharmacological Society Conference 2004",

    }

    Bibi, S, Angeli, F & Grant, MH 2004, Inhibition of glutathione reductase by chromium (VI). in Proceedings of the British Pharmacological Society Conference 2004.

    Inhibition of glutathione reductase by chromium (VI). / Bibi, S.; Angeli, F.; Grant, M.H.

    Proceedings of the British Pharmacological Society Conference 2004. 2004.

    Research output: Chapter in Book/Report/Conference proceedingChapter

    TY - CHAP

    T1 - Inhibition of glutathione reductase by chromium (VI)

    AU - Bibi, S.

    AU - Angeli, F.

    AU - Grant, M.H.

    PY - 2004

    Y1 - 2004

    N2 - Chromium (VI) compounds have serious toxic and carcinogenic effects in humans. To exert toxicity and carcinogenicity Cr (VI) must be reduced inside cells, and it can be reduced both enzymatically and non-enyzmatically. Glutathione reductase (GR) has been implicated in the intracellular reduction of Cr (VI). This enzyme normally re-cycles reduced glutathione (GSH) from oxidized glutathione (GSSG), and is essential for protecting cells against intermediates which deplete GSH, and against oxidative stress. During its reduction inside cells Cr (VI) disrupts redox balance, and generates reactive oxygen species. These oxidize GSH and deplete reduced thiols, leading to toxicity and carcinogenesis. Susceptibility to Cr (VI) will depend on the ability of cells to protect themselves by recycling GSH via GR. The effect of exposure to Cr (VI) in vitro on the activity of GR in cells derived from liver (Hep G2 cells), colon (HT 115 cells), larynx (Hep 2 cells) and macrophages (J774.1 cells) was investigated.

    AB - Chromium (VI) compounds have serious toxic and carcinogenic effects in humans. To exert toxicity and carcinogenicity Cr (VI) must be reduced inside cells, and it can be reduced both enzymatically and non-enyzmatically. Glutathione reductase (GR) has been implicated in the intracellular reduction of Cr (VI). This enzyme normally re-cycles reduced glutathione (GSH) from oxidized glutathione (GSSG), and is essential for protecting cells against intermediates which deplete GSH, and against oxidative stress. During its reduction inside cells Cr (VI) disrupts redox balance, and generates reactive oxygen species. These oxidize GSH and deplete reduced thiols, leading to toxicity and carcinogenesis. Susceptibility to Cr (VI) will depend on the ability of cells to protect themselves by recycling GSH via GR. The effect of exposure to Cr (VI) in vitro on the activity of GR in cells derived from liver (Hep G2 cells), colon (HT 115 cells), larynx (Hep 2 cells) and macrophages (J774.1 cells) was investigated.

    KW - bioengineering

    KW - glutathione reductase

    KW - chromium

    KW - pharmacology

    UR - http://www.pa2online.org/

    UR - http://www.pa2online.org/Vol2Issue4abst139P.pdf

    M3 - Chapter

    BT - Proceedings of the British Pharmacological Society Conference 2004

    ER -

    Bibi S, Angeli F, Grant MH. Inhibition of glutathione reductase by chromium (VI). In Proceedings of the British Pharmacological Society Conference 2004. 2004