Inhibition of cystathionine beta‐synthase (CBS) decreases acetylcholine mediated relaxation in murine aorta and mesenteric arteries

Hicham Labazi, Brandi Michele Wynne, R Clinton Webb

Research output: Contribution to journalConference abstractpeer-review

Abstract

Hydrogen sulfide (H2S) is a novel gas involved in the regulation of blood pressure and vascular function, exhibiting a potent vasorelaxant effect alongside nitric oxide (NO) and carbon monoxide (CO). H2S is generated endogenously from cysteine by three enzymes: cystathionine beta-synthase (CBS), cystathionine gamma-lyase (CSE) and 3-mercaptopyruvate sulfurtransferase (3-MST). Although predominant interest has been given to the role of CSE in the cardiovascular system, the role of CBS has been poorly investigated. We hypothesize that H2S generation by CBS plays an important role in vascular function, and the inhibition of this enzyme leads to decreased relaxation. In aorta, Inhibition of CBS using amino oxyacetic acid (AOAA, 3 mM) leads to a significant decrease in sensitivity (EC50) to acetylcholine (ACh); however, it did not affect the maximal relaxation. In mesenteric artery, inhibition of CBS leads to a significant decrease in sensitivity and maximal relaxation response to Ach. Inhibition of CBS did not affect sodium nitroprusside (SNP) mediated relaxation in either aorta or mesenteric arteries. These data suggest that CBS plays an important role in the regulation of vascular tone and that CBS inhibition leads to decreased endothelium dependant-relaxation.
Original languageEnglish
Pages (from-to)643.26-643.26
Number of pages1
JournalFASEB Journal
Volume25
Issue numberS1
DOIs
Publication statusPublished - 1 Apr 2011

Keywords

  • mesenteric arteries
  • cystathionine beta-synthase (CBS)
  • murine aorta
  • blood pressure
  • vascular function
  • acetylcholine mediated relaxation
  • cardiovascular system
  • amino oxyacetic acid (AOAA, 3 mM)

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