Infrared based saliva screening test for COVID-19

Bayden Robert Wood; Kamila Kochan; Diana E. Bedolla; Natalia Salazar-Quiroz; Samantha Grimley; David Perez-Guaita; Matthew J. Baker; Jitraporn Vongsvivut; Mark Tobin; Keith Bambery; Dale Christensen; Shivani Pasricha; Anthony K. Eden; Aaron Mclean; Supti Roy; Jason Roberts; Julian Druce; Deborah A. Williamson; Julie McAuley; Mike Catton; Damian Purcell; Dale Godfrey; Philip Heruad

doi:10.1002/anie.202104453

Infrared based saliva screening test for COVID-19

Bayden Robert Wood, Kamila Kochan, Diana E. Bedolla, Natalia Salazar-Quiroz, Samantha Grimley, David Perez-Guaita, Matthew J. Baker, Jitraporn Vongsvivut, Mark Tobin, Keith Bambery, Dale Christensen, Shivani Pasricha, Anthony K. Eden, Aaron Mclean, Supti Roy, Jason Roberts, Julian Druce, Deborah A. Williamson, Julie McAuley, Mike CattonDamian Purcell, Dale Godfrey, Philip Heruad

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Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in an unprecedented need for diagnostic testing that is critical in controlling the spread of COVID-19. We propose a portable infrared spectrometer with purpose-built transflection accessory for rapid point-of-care detection of COVID-19 markers in saliva. Initially, purified virion particles were characterized with Raman spectroscopy, synchrotron infrared (IR) and AFM-IR. A data set comprising 171 transflection infrared spectra from 29 subjects testing positive for SARS-CoV-2 by RT-qPCR and 28 testing negative, was modeled using Monte Carlo Double Cross Validation with 50 randomized test and model sets. The testing sensitivity was 93 % (27/29) with a specificity of 82 % (23/28) that included positive samples on the limit of detection for RT-qPCR. Herein, we demonstrate a proof-of-concept high throughput infrared COVID-19 test that is rapid, inexpensive, portable and utilizes sample self-collection thus minimizing the risk to healthcare workers and ideally suited to mass screening.

Original language	English
Pages (from-to)	17102-17107
Number of pages	6
Journal	Angewandte Chemie International Edition
Volume	60
Issue number	31
Early online date	27 May 2021
DOIs	https://doi.org/10.1002/anie.202104453
Publication status	Published - 26 Jul 2021

Keywords

covid-19 diagnostic
infrared spectroscopy
Raman spectroscopy
saliva
SARS-CoV-2

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10.1002/anie.202104453

Wood-etal-ACIE-2021-Infrared-based-saliva-screening-test-for-COVID-19Accepted author manuscript, 1.93 MB

Dataset and Data analysis for "Infrared based saliva screening test for COVID-19"
Wood, B. R. (Creator), Kochan, K. (Contributor), Bedolla, D. E. (Contributor), Perez-Guaita, D. (Contributor), Baker, M. J. (Contributor), Vongsvivut, J. (Contributor), Tobin, M. J. (Contributor), Bambery, K. R. (Contributor), Christensen, D. (Contributor), Pasricha, S. (Contributor), Eden, A. K. (Contributor), Mclean, A. (Contributor), Roy, S. (Contributor), Roberts, J. A. (Contributor), Druce, J. (Contributor), Williamson, D. A. (Contributor), McAuley, J. (Contributor), Catton, M. (Contributor), Purcell, D. F. J. (Contributor), Godfrey, D. I. (Contributor) & Heraud, P. (Contributor), Zenodo, 16 Mar 2023
DOI: 10.5281/zenodo.4156647
Dataset

Cite this

Wood, B. R., Kochan, K., Bedolla, D. E., Salazar-Quiroz, N., Grimley, S., Perez-Guaita, D., Baker, M. J., Vongsvivut, J., Tobin, M., Bambery, K., Christensen, D., Pasricha, S., Eden, A. K., Mclean, A., Roy, S., Roberts, J., Druce, J., Williamson, D. A., McAuley, J., ... Heruad, P. (2021). Infrared based saliva screening test for COVID-19. Angewandte Chemie International Edition, 60(31), 17102-17107. https://doi.org/10.1002/anie.202104453

@article{285628c25f834dfc86eb3f5cf3d927c8,

title = "Infrared based saliva screening test for COVID-19",

abstract = "Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in an unprecedented need for diagnostic testing that is critical in controlling the spread of COVID-19. We propose a portable infrared spectrometer with purpose-built transflection accessory for rapid point-of-care detection of COVID-19 markers in saliva. Initially, purified virion particles were characterized with Raman spectroscopy, synchrotron infrared (IR) and AFM-IR. A data set comprising 171 transflection infrared spectra from 29 subjects testing positive for SARS-CoV-2 by RT-qPCR and 28 testing negative, was modeled using Monte Carlo Double Cross Validation with 50 randomized test and model sets. The testing sensitivity was 93 % (27/29) with a specificity of 82 % (23/28) that included positive samples on the limit of detection for RT-qPCR. Herein, we demonstrate a proof-of-concept high throughput infrared COVID-19 test that is rapid, inexpensive, portable and utilizes sample self-collection thus minimizing the risk to healthcare workers and ideally suited to mass screening.",

keywords = "covid-19 diagnostic, infrared spectroscopy, Raman spectroscopy, saliva, SARS-CoV-2",

author = "Wood, {Bayden Robert} and Kamila Kochan and Bedolla, {Diana E.} and Natalia Salazar-Quiroz and Samantha Grimley and David Perez-Guaita and Baker, {Matthew J.} and Jitraporn Vongsvivut and Mark Tobin and Keith Bambery and Dale Christensen and Shivani Pasricha and Eden, {Anthony K.} and Aaron Mclean and Supti Roy and Jason Roberts and Julian Druce and Williamson, {Deborah A.} and Julie McAuley and Mike Catton and Damian Purcell and Dale Godfrey and Philip Heruad",

year = "2021",

month = jul,

day = "26",

doi = "10.1002/anie.202104453",

language = "English",

volume = "60",

pages = "17102--17107",

journal = "Angewandte Chemie International Edition",

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Wood, BR, Kochan, K, Bedolla, DE, Salazar-Quiroz, N, Grimley, S, Perez-Guaita, D, Baker, MJ, Vongsvivut, J, Tobin, M, Bambery, K, Christensen, D, Pasricha, S, Eden, AK, Mclean, A, Roy, S, Roberts, J, Druce, J, Williamson, DA, McAuley, J, Catton, M, Purcell, D, Godfrey, D & Heruad, P 2021, 'Infrared based saliva screening test for COVID-19', Angewandte Chemie International Edition, vol. 60, no. 31, pp. 17102-17107. https://doi.org/10.1002/anie.202104453

TY - JOUR

T1 - Infrared based saliva screening test for COVID-19

AU - Wood, Bayden Robert

AU - Kochan, Kamila

AU - Bedolla, Diana E.

AU - Salazar-Quiroz, Natalia

AU - Grimley, Samantha

AU - Perez-Guaita, David

AU - Baker, Matthew J.

AU - Vongsvivut, Jitraporn

AU - Tobin, Mark

AU - Bambery, Keith

AU - Christensen, Dale

AU - Pasricha, Shivani

AU - Eden, Anthony K.

AU - Mclean, Aaron

AU - Roy, Supti

AU - Roberts, Jason

AU - Druce, Julian

AU - Williamson, Deborah A.

AU - McAuley, Julie

AU - Catton, Mike

AU - Purcell, Damian

AU - Godfrey, Dale

AU - Heruad, Philip

PY - 2021/7/26

Y1 - 2021/7/26

N2 - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in an unprecedented need for diagnostic testing that is critical in controlling the spread of COVID-19. We propose a portable infrared spectrometer with purpose-built transflection accessory for rapid point-of-care detection of COVID-19 markers in saliva. Initially, purified virion particles were characterized with Raman spectroscopy, synchrotron infrared (IR) and AFM-IR. A data set comprising 171 transflection infrared spectra from 29 subjects testing positive for SARS-CoV-2 by RT-qPCR and 28 testing negative, was modeled using Monte Carlo Double Cross Validation with 50 randomized test and model sets. The testing sensitivity was 93 % (27/29) with a specificity of 82 % (23/28) that included positive samples on the limit of detection for RT-qPCR. Herein, we demonstrate a proof-of-concept high throughput infrared COVID-19 test that is rapid, inexpensive, portable and utilizes sample self-collection thus minimizing the risk to healthcare workers and ideally suited to mass screening.

AB - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in an unprecedented need for diagnostic testing that is critical in controlling the spread of COVID-19. We propose a portable infrared spectrometer with purpose-built transflection accessory for rapid point-of-care detection of COVID-19 markers in saliva. Initially, purified virion particles were characterized with Raman spectroscopy, synchrotron infrared (IR) and AFM-IR. A data set comprising 171 transflection infrared spectra from 29 subjects testing positive for SARS-CoV-2 by RT-qPCR and 28 testing negative, was modeled using Monte Carlo Double Cross Validation with 50 randomized test and model sets. The testing sensitivity was 93 % (27/29) with a specificity of 82 % (23/28) that included positive samples on the limit of detection for RT-qPCR. Herein, we demonstrate a proof-of-concept high throughput infrared COVID-19 test that is rapid, inexpensive, portable and utilizes sample self-collection thus minimizing the risk to healthcare workers and ideally suited to mass screening.

KW - covid-19 diagnostic

KW - infrared spectroscopy

KW - Raman spectroscopy

KW - saliva

KW - SARS-CoV-2

U2 - 10.1002/anie.202104453

DO - 10.1002/anie.202104453

M3 - Article

SN - 1433-7851

VL - 60

SP - 17102

EP - 17107

JO - Angewandte Chemie International Edition

JF - Angewandte Chemie International Edition

IS - 31

ER -

Infrared based saliva screening test for COVID-19

Abstract

Keywords

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Dataset and Data analysis for "Infrared based saliva screening test for COVID-19"

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