Infrared based saliva screening test for COVID-19

Bayden Robert Wood, Kamila Kochan, Diana E. Bedolla, Natalia Salazar-Quiroz, Samantha Grimley, David Perez-Guaita, Matthew J. Baker, Jitraporn Vongsvivut, Mark Tobin, Keith Bambery, Dale Christensen, Shivani Pasricha, Anthony K. Eden, Aaron Mclean, Supti Roy, Jason Roberts, Julian Druce, Deborah A. Williamson, Julie McAuley, Mike CattonDamian Purcell, Dale Godfrey, Philip Heruad

Research output: Contribution to journalArticlepeer-review

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in an unprecedented need for diagnostic testing that is critical in controlling the spread of COVID-19. We propose a portable infrared spectrometer with purpose-built transflection accessory for rapid point-of-care detection of COVID-19 markers in saliva. Initially, purified virion particles were characterized with Raman spectroscopy, synchrotron infrared (IR) and AFM-IR. A data set comprising 171 transflection infrared spectra from 29 subjects testing positive for SARS-CoV-2 by RT-qPCR and 28 testing negative, was modeled using Monte Carlo Double Cross Validation with 50 randomized test and model sets. The testing sensitivity was 93 % (27/29) with a specificity of 82 % (23/28) that included positive samples on the limit of detection for RT-qPCR. Herein, we demonstrate a proof-of-concept high throughput infrared COVID-19 test that is rapid, inexpensive, portable and utilizes sample self-collection thus minimizing the risk to healthcare workers and ideally suited to mass screening.

Original languageEnglish
JournalAngewandte Chemie International Edition
Early online date27 May 2021
DOIs
Publication statusE-pub ahead of print - 27 May 2021

Keywords

  • covid-19 diagnostic
  • infrared spectroscopy
  • Raman spectroscopy
  • saliva
  • SARS-CoV-2

Fingerprint

Dive into the research topics of 'Infrared based saliva screening test for COVID-19'. Together they form a unique fingerprint.

Cite this