Influence of cultivation for growth of listeria monocytogenes under different temperature conditions

Research output: Contribution to conferencePoster

Abstract

This study compared the performance of various methods for cultivating Listeria monocytogenes in broth medium (Tryptone soya broth with 0.6 % yeast extract). The cultivation methods compared were shaken flask, static flask and a 2.5 litre stirred bioreactor system. Strains NCTC 10357 (serotype 1a) and NCTC 11994 (serotype 4b) were investigated using shaken and static flasks at 37 °C using both the spiral plate method and the conventional plate method for growth assessment. Results demonstrated that the spiral plate enumeration method produced more consistent results and smoother growth curves than the conventional dilution plate method.

Conference

ConferenceSociety for General Microbiology, 155th Meeting
CitySociety for General Microbiology
Period6/09/049/09/04

Fingerprint

temperature
assessment method
yeast
dilution
method
cultivation method

Keywords

  • cultivation methods
  • listeria monocytogenes
  • temperature conditions

Cite this

Lani, M. N., Anderson, J. G., & MacGregor, S. J. (2004). Influence of cultivation for growth of listeria monocytogenes under different temperature conditions. Poster session presented at Society for General Microbiology, 155th Meeting, Society for General Microbiology, .
Lani, M.N. ; Anderson, J.G. ; MacGregor, S.J. / Influence of cultivation for growth of listeria monocytogenes under different temperature conditions. Poster session presented at Society for General Microbiology, 155th Meeting, Society for General Microbiology, .
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title = "Influence of cultivation for growth of listeria monocytogenes under different temperature conditions",
abstract = "This study compared the performance of various methods for cultivating Listeria monocytogenes in broth medium (Tryptone soya broth with 0.6 {\%} yeast extract). The cultivation methods compared were shaken flask, static flask and a 2.5 litre stirred bioreactor system. Strains NCTC 10357 (serotype 1a) and NCTC 11994 (serotype 4b) were investigated using shaken and static flasks at 37 °C using both the spiral plate method and the conventional plate method for growth assessment. Results demonstrated that the spiral plate enumeration method produced more consistent results and smoother growth curves than the conventional dilution plate method.",
keywords = "cultivation methods, listeria monocytogenes, temperature conditions",
author = "M.N. Lani and J.G. Anderson and S.J. MacGregor",
note = "Abstract can be found as item no: FB 15, page 61 of http://www.sgm.ac.uk/meetings/pdfabstracts/tcd2004abs.pdf; Society for General Microbiology, 155th Meeting ; Conference date: 06-09-2004 Through 09-09-2004",
year = "2004",
month = "9",
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language = "English",

}

Lani, MN, Anderson, JG & MacGregor, SJ 2004, 'Influence of cultivation for growth of listeria monocytogenes under different temperature conditions' Society for General Microbiology, 155th Meeting, Society for General Microbiology, 6/09/04 - 9/09/04, .

Influence of cultivation for growth of listeria monocytogenes under different temperature conditions. / Lani, M.N.; Anderson, J.G.; MacGregor, S.J.

2004. Poster session presented at Society for General Microbiology, 155th Meeting, Society for General Microbiology, .

Research output: Contribution to conferencePoster

TY - CONF

T1 - Influence of cultivation for growth of listeria monocytogenes under different temperature conditions

AU - Lani, M.N.

AU - Anderson, J.G.

AU - MacGregor, S.J.

N1 - Abstract can be found as item no: FB 15, page 61 of http://www.sgm.ac.uk/meetings/pdfabstracts/tcd2004abs.pdf

PY - 2004/9/6

Y1 - 2004/9/6

N2 - This study compared the performance of various methods for cultivating Listeria monocytogenes in broth medium (Tryptone soya broth with 0.6 % yeast extract). The cultivation methods compared were shaken flask, static flask and a 2.5 litre stirred bioreactor system. Strains NCTC 10357 (serotype 1a) and NCTC 11994 (serotype 4b) were investigated using shaken and static flasks at 37 °C using both the spiral plate method and the conventional plate method for growth assessment. Results demonstrated that the spiral plate enumeration method produced more consistent results and smoother growth curves than the conventional dilution plate method.

AB - This study compared the performance of various methods for cultivating Listeria monocytogenes in broth medium (Tryptone soya broth with 0.6 % yeast extract). The cultivation methods compared were shaken flask, static flask and a 2.5 litre stirred bioreactor system. Strains NCTC 10357 (serotype 1a) and NCTC 11994 (serotype 4b) were investigated using shaken and static flasks at 37 °C using both the spiral plate method and the conventional plate method for growth assessment. Results demonstrated that the spiral plate enumeration method produced more consistent results and smoother growth curves than the conventional dilution plate method.

KW - cultivation methods

KW - listeria monocytogenes

KW - temperature conditions

UR - http://www.sgm.ac.uk/meetings/pdfabstracts/tcd2004abs.pdf

M3 - Poster

ER -

Lani MN, Anderson JG, MacGregor SJ. Influence of cultivation for growth of listeria monocytogenes under different temperature conditions. 2004. Poster session presented at Society for General Microbiology, 155th Meeting, Society for General Microbiology, .