Influence of cultivation for growth of listeria monocytogenes under different temperature conditions

M.N. Lani; J.G. Anderson; S.J. MacGregor

Influence of cultivation for growth of listeria monocytogenes under different temperature conditions

M.N. Lani, J.G. Anderson, S.J. MacGregor

Electronic And Electrical Engineering

Research output: Contribution to conference › Poster

Abstract

This study compared the performance of various methods for cultivating Listeria monocytogenes in broth medium (Tryptone soya broth with 0.6 % yeast extract). The cultivation methods compared were shaken flask, static flask and a 2.5 litre stirred bioreactor system. Strains NCTC 10357 (serotype 1a) and NCTC 11994 (serotype 4b) were investigated using shaken and static flasks at 37 °C using both the spiral plate method and the conventional plate method for growth assessment. Results demonstrated that the spiral plate enumeration method produced more consistent results and smoother growth curves than the conventional dilution plate method.

Original language	English
Publication status	Published - 6 Sep 2004
Event	Society for General Microbiology, 155th Meeting - Society for General Microbiology Duration: 6 Sep 2004 → 9 Sep 2004

Conference

Conference	Society for General Microbiology, 155th Meeting
City	Society for General Microbiology
Period	6/09/04 → 9/09/04

Keywords

cultivation methods
listeria monocytogenes
temperature conditions

Access to Document

http://www.sgm.ac.uk/meetings/pdfabstracts/tcd2004abs.pdf

Cite this

@conference{1a896b0b474445b69350a48e2cfeba6b,

title = "Influence of cultivation for growth of listeria monocytogenes under different temperature conditions",

abstract = "This study compared the performance of various methods for cultivating Listeria monocytogenes in broth medium (Tryptone soya broth with 0.6 % yeast extract). The cultivation methods compared were shaken flask, static flask and a 2.5 litre stirred bioreactor system. Strains NCTC 10357 (serotype 1a) and NCTC 11994 (serotype 4b) were investigated using shaken and static flasks at 37 °C using both the spiral plate method and the conventional plate method for growth assessment. Results demonstrated that the spiral plate enumeration method produced more consistent results and smoother growth curves than the conventional dilution plate method.",

keywords = "cultivation methods, listeria monocytogenes, temperature conditions",

author = "M.N. Lani and J.G. Anderson and S.J. MacGregor",

note = "Abstract can be found as item no: FB 15, page 61 of http://www.sgm.ac.uk/meetings/pdfabstracts/tcd2004abs.pdf; Society for General Microbiology, 155th Meeting ; Conference date: 06-09-2004 Through 09-09-2004",

year = "2004",

month = sep,

day = "6",

language = "English",

}

TY - CONF

T1 - Influence of cultivation for growth of listeria monocytogenes under different temperature conditions

AU - Lani, M.N.

AU - Anderson, J.G.

AU - MacGregor, S.J.

N1 - Abstract can be found as item no: FB 15, page 61 of http://www.sgm.ac.uk/meetings/pdfabstracts/tcd2004abs.pdf

PY - 2004/9/6

Y1 - 2004/9/6

N2 - This study compared the performance of various methods for cultivating Listeria monocytogenes in broth medium (Tryptone soya broth with 0.6 % yeast extract). The cultivation methods compared were shaken flask, static flask and a 2.5 litre stirred bioreactor system. Strains NCTC 10357 (serotype 1a) and NCTC 11994 (serotype 4b) were investigated using shaken and static flasks at 37 °C using both the spiral plate method and the conventional plate method for growth assessment. Results demonstrated that the spiral plate enumeration method produced more consistent results and smoother growth curves than the conventional dilution plate method.

AB - This study compared the performance of various methods for cultivating Listeria monocytogenes in broth medium (Tryptone soya broth with 0.6 % yeast extract). The cultivation methods compared were shaken flask, static flask and a 2.5 litre stirred bioreactor system. Strains NCTC 10357 (serotype 1a) and NCTC 11994 (serotype 4b) were investigated using shaken and static flasks at 37 °C using both the spiral plate method and the conventional plate method for growth assessment. Results demonstrated that the spiral plate enumeration method produced more consistent results and smoother growth curves than the conventional dilution plate method.

KW - cultivation methods

KW - listeria monocytogenes

KW - temperature conditions

UR - http://www.sgm.ac.uk/meetings/pdfabstracts/tcd2004abs.pdf

M3 - Poster

T2 - Society for General Microbiology, 155th Meeting

Y2 - 6 September 2004 through 9 September 2004

ER -