Abstract
This study compared the performance of various methods for
cultivating Listeria monocytogenes in broth medium (Tryptone soya
broth with 0.6 % yeast extract). The cultivation methods compared
were shaken flask, static flask and a 2.5 litre stirred bioreactor
system. Strains NCTC 10357 (serotype 1a) and NCTC 11994 (serotype
4b) were investigated using shaken and static flasks at 37 °C using
both the spiral plate method and the conventional plate method for
growth assessment. Results demonstrated that the spiral plate
enumeration method produced more consistent results and smoother
growth curves than the conventional dilution plate method.
Original language | English |
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Publication status | Published - 6 Sept 2004 |
Event | Society for General Microbiology, 155th Meeting - Society for General Microbiology Duration: 6 Sept 2004 → 9 Sept 2004 |
Conference
Conference | Society for General Microbiology, 155th Meeting |
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City | Society for General Microbiology |
Period | 6/09/04 → 9/09/04 |
Keywords
- cultivation methods
- listeria monocytogenes
- temperature conditions