Influence of 100% and 40% oxygen on penumbral blood flow, oxygen level, and T2*-weighted MRI in a rat stroke model

Tracey A Baskerville, Graeme A Deuchar, Christopher McCabe, Craig A Robertson, William M Holmes, Celestine Santosh, I Mhairi Macrae

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Accurate imaging of the ischemic penumbra is a prerequisite for acute clinical stroke research. T(2)(*) magnetic resonance imaging (MRI) combined with an oxygen challenge (OC) is being developed to detect penumbra based on changes in blood deoxyhemoglobin. However, inducing OC with 100% O(2) induces sinus artefacts on human scans and influences cerebral blood flow (CBF), which can affect T(2)(*) signal. Therefore, we investigated replacing 100% O(2) OC with 40% O(2) OC (5 minutes 40% O(2) versus 100% O(2)) and determined the effects on blood pressure (BP), CBF, tissue pO(2), and T(2)(*) signal change in presumed penumbra in a rat stroke model. Probes implanted into penumbra and contralateral cortex simultaneously recorded pO(2) and CBF during 40% O(2) (n=6) or 100% O(2) (n=8) OC. In a separate MRI study, T(2)(*) signal change to 40% O(2) (n=6) and 100% O(2) (n=5) OC was compared. Oxygen challenge (40% and 100% O(2)) increased BP by 8.2% and 18.1%, penumbra CBF by 5% and 15%, and penumbra pO(2) levels by 80% and 144%, respectively. T(2)(*) signal significantly increased by 4.56% ± 1.61% and 8.65% ± 3.66% in penumbra compared with 2.98% ± 1.56% and 2.79% ± 0.66% in contralateral cortex and 1.09% ± 0.82% and -0.32% ± 0.67% in ischemic core, respectively. For diagnostic imaging, 40% O(2) OC could provide sufficient T(2)(*) signal change to detect penumbra with limited influence in BP and CBF.

LanguageEnglish
Pages1799-1806
Number of pages8
JournalJournal of Cerebral Blood Flow and Metabolism
Volume31
Issue number8
Early online date11 May 2011
DOIs
Publication statusPublished - Aug 2011

Fingerprint

Magnetic resonance
Cerebrovascular Circulation
Rats
Blood
Stroke
Magnetic Resonance Imaging
Oxygen
Imaging techniques
Blood pressure
Blood Pressure
Diagnostic Imaging
Artifacts
Tissue
Research

Keywords

  • animals
  • blood pressure
  • cerebrovascular circulation
  • diagnostic imaging
  • disease models, animal
  • magnetic resonance imaging
  • oxygen
  • rats
  • stroke

Cite this

Baskerville, Tracey A ; Deuchar, Graeme A ; McCabe, Christopher ; Robertson, Craig A ; Holmes, William M ; Santosh, Celestine ; Macrae, I Mhairi. / Influence of 100% and 40% oxygen on penumbral blood flow, oxygen level, and T2*-weighted MRI in a rat stroke model. In: Journal of Cerebral Blood Flow and Metabolism. 2011 ; Vol. 31, No. 8. pp. 1799-1806.
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abstract = "Accurate imaging of the ischemic penumbra is a prerequisite for acute clinical stroke research. T(2)(*) magnetic resonance imaging (MRI) combined with an oxygen challenge (OC) is being developed to detect penumbra based on changes in blood deoxyhemoglobin. However, inducing OC with 100{\%} O(2) induces sinus artefacts on human scans and influences cerebral blood flow (CBF), which can affect T(2)(*) signal. Therefore, we investigated replacing 100{\%} O(2) OC with 40{\%} O(2) OC (5 minutes 40{\%} O(2) versus 100{\%} O(2)) and determined the effects on blood pressure (BP), CBF, tissue pO(2), and T(2)(*) signal change in presumed penumbra in a rat stroke model. Probes implanted into penumbra and contralateral cortex simultaneously recorded pO(2) and CBF during 40{\%} O(2) (n=6) or 100{\%} O(2) (n=8) OC. In a separate MRI study, T(2)(*) signal change to 40{\%} O(2) (n=6) and 100{\%} O(2) (n=5) OC was compared. Oxygen challenge (40{\%} and 100{\%} O(2)) increased BP by 8.2{\%} and 18.1{\%}, penumbra CBF by 5{\%} and 15{\%}, and penumbra pO(2) levels by 80{\%} and 144{\%}, respectively. T(2)(*) signal significantly increased by 4.56{\%} ± 1.61{\%} and 8.65{\%} ± 3.66{\%} in penumbra compared with 2.98{\%} ± 1.56{\%} and 2.79{\%} ± 0.66{\%} in contralateral cortex and 1.09{\%} ± 0.82{\%} and -0.32{\%} ± 0.67{\%} in ischemic core, respectively. For diagnostic imaging, 40{\%} O(2) OC could provide sufficient T(2)(*) signal change to detect penumbra with limited influence in BP and CBF.",
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Influence of 100% and 40% oxygen on penumbral blood flow, oxygen level, and T2*-weighted MRI in a rat stroke model. / Baskerville, Tracey A; Deuchar, Graeme A; McCabe, Christopher; Robertson, Craig A; Holmes, William M; Santosh, Celestine; Macrae, I Mhairi.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 31, No. 8, 08.2011, p. 1799-1806.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Influence of 100% and 40% oxygen on penumbral blood flow, oxygen level, and T2*-weighted MRI in a rat stroke model

AU - Baskerville, Tracey A

AU - Deuchar, Graeme A

AU - McCabe, Christopher

AU - Robertson, Craig A

AU - Holmes, William M

AU - Santosh, Celestine

AU - Macrae, I Mhairi

PY - 2011/8

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N2 - Accurate imaging of the ischemic penumbra is a prerequisite for acute clinical stroke research. T(2)(*) magnetic resonance imaging (MRI) combined with an oxygen challenge (OC) is being developed to detect penumbra based on changes in blood deoxyhemoglobin. However, inducing OC with 100% O(2) induces sinus artefacts on human scans and influences cerebral blood flow (CBF), which can affect T(2)(*) signal. Therefore, we investigated replacing 100% O(2) OC with 40% O(2) OC (5 minutes 40% O(2) versus 100% O(2)) and determined the effects on blood pressure (BP), CBF, tissue pO(2), and T(2)(*) signal change in presumed penumbra in a rat stroke model. Probes implanted into penumbra and contralateral cortex simultaneously recorded pO(2) and CBF during 40% O(2) (n=6) or 100% O(2) (n=8) OC. In a separate MRI study, T(2)(*) signal change to 40% O(2) (n=6) and 100% O(2) (n=5) OC was compared. Oxygen challenge (40% and 100% O(2)) increased BP by 8.2% and 18.1%, penumbra CBF by 5% and 15%, and penumbra pO(2) levels by 80% and 144%, respectively. T(2)(*) signal significantly increased by 4.56% ± 1.61% and 8.65% ± 3.66% in penumbra compared with 2.98% ± 1.56% and 2.79% ± 0.66% in contralateral cortex and 1.09% ± 0.82% and -0.32% ± 0.67% in ischemic core, respectively. For diagnostic imaging, 40% O(2) OC could provide sufficient T(2)(*) signal change to detect penumbra with limited influence in BP and CBF.

AB - Accurate imaging of the ischemic penumbra is a prerequisite for acute clinical stroke research. T(2)(*) magnetic resonance imaging (MRI) combined with an oxygen challenge (OC) is being developed to detect penumbra based on changes in blood deoxyhemoglobin. However, inducing OC with 100% O(2) induces sinus artefacts on human scans and influences cerebral blood flow (CBF), which can affect T(2)(*) signal. Therefore, we investigated replacing 100% O(2) OC with 40% O(2) OC (5 minutes 40% O(2) versus 100% O(2)) and determined the effects on blood pressure (BP), CBF, tissue pO(2), and T(2)(*) signal change in presumed penumbra in a rat stroke model. Probes implanted into penumbra and contralateral cortex simultaneously recorded pO(2) and CBF during 40% O(2) (n=6) or 100% O(2) (n=8) OC. In a separate MRI study, T(2)(*) signal change to 40% O(2) (n=6) and 100% O(2) (n=5) OC was compared. Oxygen challenge (40% and 100% O(2)) increased BP by 8.2% and 18.1%, penumbra CBF by 5% and 15%, and penumbra pO(2) levels by 80% and 144%, respectively. T(2)(*) signal significantly increased by 4.56% ± 1.61% and 8.65% ± 3.66% in penumbra compared with 2.98% ± 1.56% and 2.79% ± 0.66% in contralateral cortex and 1.09% ± 0.82% and -0.32% ± 0.67% in ischemic core, respectively. For diagnostic imaging, 40% O(2) OC could provide sufficient T(2)(*) signal change to detect penumbra with limited influence in BP and CBF.

KW - animals

KW - blood pressure

KW - cerebrovascular circulation

KW - diagnostic imaging

KW - disease models, animal

KW - magnetic resonance imaging

KW - oxygen

KW - rats

KW - stroke

U2 - 10.1038/jcbfm.2011.65

DO - 10.1038/jcbfm.2011.65

M3 - Article

VL - 31

SP - 1799

EP - 1806

JO - Journal of Cerebral Blood Flow and Metabolism

T2 - Journal of Cerebral Blood Flow and Metabolism

JF - Journal of Cerebral Blood Flow and Metabolism

SN - 0271-678X

IS - 8

ER -