Induced encystment improves resistance to preservation and storage of Acanthamoeba castellanii

S.J. Campbell, P.R. Ingram, C.W. Roberts, F.L. Henriquez

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Several conditions that allow the preservation, storage and rapid, efficient recovery of viable Acanthamoeba castellanii organisms were investigated. The viability of trophozoites (as determined by time to confluence) significantly declined over a period of 12 months when stored at −70°C using dimethyl sulfoxide (DMSO; 5 or 10%) as cryopreservant. As A. castellanii are naturally capable of encystment, studies were undertaken to determine whether induced encystment might improve the viability of organisms under a number of storage conditions. A. castellanii cysts stored in the presence of Mg2+ at 4°C remained viable over the study period, although time to confluence was increased from approximately 8 days to approximately 24 days over the 12-month period. Storage of cysts at −70°C with DMSO (5 or 10%) or 40% glycerol, but not 80% glycerol as cryopreservants increased their viability over the 12-month study period compared with those stored at room temperature. Continued presence of Mg2+ in medium during storage had no adverse effects and generally improved recovery of viable organisms. The present study demonstrates that A. castellanii can be stored as a non-multiplicative form inexpensively, without a need for cryopreservation, for at least 12 months, but viability is increased by storage at −70°C.
LanguageEnglish
Pages1401-1405
Number of pages5
JournalParasitology
Volume135
Issue number12
DOIs
Publication statusPublished - Oct 2008

Fingerprint

Acanthamoeba castellanii
encystment
Dimethyl Sulfoxide
viability
Glycerol
Cysts
glycerol
organisms
Trophozoites
trophozoites
Cryopreservation
dimethyl sulfoxide
storage conditions
cryopreservation
ambient temperature
adverse effects
Temperature

Keywords

  • acanthamoeba
  • preservation
  • storage
  • cyst
  • trophozoite

Cite this

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abstract = "Several conditions that allow the preservation, storage and rapid, efficient recovery of viable Acanthamoeba castellanii organisms were investigated. The viability of trophozoites (as determined by time to confluence) significantly declined over a period of 12 months when stored at −70°C using dimethyl sulfoxide (DMSO; 5 or 10{\%}) as cryopreservant. As A. castellanii are naturally capable of encystment, studies were undertaken to determine whether induced encystment might improve the viability of organisms under a number of storage conditions. A. castellanii cysts stored in the presence of Mg2+ at 4°C remained viable over the study period, although time to confluence was increased from approximately 8 days to approximately 24 days over the 12-month period. Storage of cysts at −70°C with DMSO (5 or 10{\%}) or 40{\%} glycerol, but not 80{\%} glycerol as cryopreservants increased their viability over the 12-month study period compared with those stored at room temperature. Continued presence of Mg2+ in medium during storage had no adverse effects and generally improved recovery of viable organisms. The present study demonstrates that A. castellanii can be stored as a non-multiplicative form inexpensively, without a need for cryopreservation, for at least 12 months, but viability is increased by storage at −70°C.",
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Induced encystment improves resistance to preservation and storage of Acanthamoeba castellanii. / Campbell, S.J.; Ingram, P.R.; Roberts, C.W.; Henriquez, F.L.

In: Parasitology, Vol. 135, No. 12, 10.2008, p. 1401-1405.

Research output: Contribution to journalArticle

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