Independent validation of body fluid-specific CpG markers and construction of a robust multiplex assay

Hannah Holtkötter, Vanessa Beyer, Kristina Schwender, Alina Glaub, Kristina Schulze Johann, Marianne Schürenkamp, Ursula Sibbing, Sabrina Banken, Peter Wiegand, Heidi Pfeiffer, Lynn Dennany, Marielle Vennamann

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Potential forensic use of tissue-specific DNA methylation markers has recently been discussed for the identification of the biological source of a stain. In this study 13 promising markers were evaluated to identify suitable candidate markers for the development of a robust and reliable multiplex assay. The results of this study suggest that a combination of only four highly informative markers will be enough for clear body fluid identification. A multiplex assay was developed for the identification of menstrual blood, saliva, semen, and venous blood. This assay was successfully applied to the identification of these body fluids in mixtures and crime scene stains. The multiplex assay aids in the identification of not only single source body fluids but also of body fluid mixtures. The main advantage of using DNA methylation assays over alternative tests is that it can be applied at a later time point in the investigative process since testing is possible even after DNA analysis.
LanguageEnglish
Pages261-268
Number of pages8
JournalForensic Science International: Genetics
Volume29
Early online date9 May 2017
DOIs
Publication statusPublished - 31 Jul 2017

Fingerprint

Body Fluids
DNA Methylation
Coloring Agents
Crime
Semen
Genetic Markers
Saliva
DNA

Keywords

  • body fluids
  • epigenetics markers
  • DNA methylation
  • multiplex
  • body fluid identification
  • crime scene stains
  • biological source

Cite this

Holtkötter, H., Beyer, V., Schwender, K., Glaub, A., Schulze Johann, K., Schürenkamp, M., ... Vennamann, M. (2017). Independent validation of body fluid-specific CpG markers and construction of a robust multiplex assay. Forensic Science International: Genetics , 29, 261-268. https://doi.org/10.1016/j.fsigen.2017.05.002
Holtkötter, Hannah ; Beyer, Vanessa ; Schwender, Kristina ; Glaub, Alina ; Schulze Johann, Kristina ; Schürenkamp, Marianne ; Sibbing, Ursula ; Banken, Sabrina ; Wiegand, Peter ; Pfeiffer, Heidi ; Dennany, Lynn ; Vennamann, Marielle. / Independent validation of body fluid-specific CpG markers and construction of a robust multiplex assay. In: Forensic Science International: Genetics . 2017 ; Vol. 29. pp. 261-268.
@article{2ab6e1707d584628b1a64a30c6f7789a,
title = "Independent validation of body fluid-specific CpG markers and construction of a robust multiplex assay",
abstract = "Potential forensic use of tissue-specific DNA methylation markers has recently been discussed for the identification of the biological source of a stain. In this study 13 promising markers were evaluated to identify suitable candidate markers for the development of a robust and reliable multiplex assay. The results of this study suggest that a combination of only four highly informative markers will be enough for clear body fluid identification. A multiplex assay was developed for the identification of menstrual blood, saliva, semen, and venous blood. This assay was successfully applied to the identification of these body fluids in mixtures and crime scene stains. The multiplex assay aids in the identification of not only single source body fluids but also of body fluid mixtures. The main advantage of using DNA methylation assays over alternative tests is that it can be applied at a later time point in the investigative process since testing is possible even after DNA analysis.",
keywords = "body fluids, epigenetics markers, DNA methylation, multiplex, body fluid identification, crime scene stains, biological source",
author = "Hannah Holtk{\"o}tter and Vanessa Beyer and Kristina Schwender and Alina Glaub and {Schulze Johann}, Kristina and Marianne Sch{\"u}renkamp and Ursula Sibbing and Sabrina Banken and Peter Wiegand and Heidi Pfeiffer and Lynn Dennany and Marielle Vennamann",
year = "2017",
month = "7",
day = "31",
doi = "10.1016/j.fsigen.2017.05.002",
language = "English",
volume = "29",
pages = "261--268",
journal = "Forensic Science International: Genetics",
issn = "1872-4973",

}

Holtkötter, H, Beyer, V, Schwender, K, Glaub, A, Schulze Johann, K, Schürenkamp, M, Sibbing, U, Banken, S, Wiegand, P, Pfeiffer, H, Dennany, L & Vennamann, M 2017, 'Independent validation of body fluid-specific CpG markers and construction of a robust multiplex assay' Forensic Science International: Genetics , vol. 29, pp. 261-268. https://doi.org/10.1016/j.fsigen.2017.05.002

Independent validation of body fluid-specific CpG markers and construction of a robust multiplex assay. / Holtkötter, Hannah; Beyer, Vanessa; Schwender, Kristina; Glaub, Alina; Schulze Johann, Kristina; Schürenkamp, Marianne; Sibbing, Ursula; Banken, Sabrina; Wiegand, Peter; Pfeiffer, Heidi; Dennany, Lynn; Vennamann, Marielle.

In: Forensic Science International: Genetics , Vol. 29, 31.07.2017, p. 261-268.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Independent validation of body fluid-specific CpG markers and construction of a robust multiplex assay

AU - Holtkötter, Hannah

AU - Beyer, Vanessa

AU - Schwender, Kristina

AU - Glaub, Alina

AU - Schulze Johann, Kristina

AU - Schürenkamp, Marianne

AU - Sibbing, Ursula

AU - Banken, Sabrina

AU - Wiegand, Peter

AU - Pfeiffer, Heidi

AU - Dennany, Lynn

AU - Vennamann, Marielle

PY - 2017/7/31

Y1 - 2017/7/31

N2 - Potential forensic use of tissue-specific DNA methylation markers has recently been discussed for the identification of the biological source of a stain. In this study 13 promising markers were evaluated to identify suitable candidate markers for the development of a robust and reliable multiplex assay. The results of this study suggest that a combination of only four highly informative markers will be enough for clear body fluid identification. A multiplex assay was developed for the identification of menstrual blood, saliva, semen, and venous blood. This assay was successfully applied to the identification of these body fluids in mixtures and crime scene stains. The multiplex assay aids in the identification of not only single source body fluids but also of body fluid mixtures. The main advantage of using DNA methylation assays over alternative tests is that it can be applied at a later time point in the investigative process since testing is possible even after DNA analysis.

AB - Potential forensic use of tissue-specific DNA methylation markers has recently been discussed for the identification of the biological source of a stain. In this study 13 promising markers were evaluated to identify suitable candidate markers for the development of a robust and reliable multiplex assay. The results of this study suggest that a combination of only four highly informative markers will be enough for clear body fluid identification. A multiplex assay was developed for the identification of menstrual blood, saliva, semen, and venous blood. This assay was successfully applied to the identification of these body fluids in mixtures and crime scene stains. The multiplex assay aids in the identification of not only single source body fluids but also of body fluid mixtures. The main advantage of using DNA methylation assays over alternative tests is that it can be applied at a later time point in the investigative process since testing is possible even after DNA analysis.

KW - body fluids

KW - epigenetics markers

KW - DNA methylation

KW - multiplex

KW - body fluid identification

KW - crime scene stains

KW - biological source

U2 - 10.1016/j.fsigen.2017.05.002

DO - 10.1016/j.fsigen.2017.05.002

M3 - Article

VL - 29

SP - 261

EP - 268

JO - Forensic Science International: Genetics

T2 - Forensic Science International: Genetics

JF - Forensic Science International: Genetics

SN - 1872-4973

ER -