Increased VO2max does not influence nitric oxide availability or the response to a nitrate dose

Chris Easton, David Muggeridge, Gareth Willis, Philip E. James

Research output: Contribution to conferenceAbstract

Abstract

Dietary nitrate (NIT) supplementation increases nitric oxide (NO) metabolites and may improve exercise performance. However, NIT supplementation seems to be less efficacious in highly trained athletes. Despite this, no study has directly investigated the effects of a change in aerobic capacity (VO2max) on baseline plasma NO metabolites or the response to a NIT dose within the same cohort.PURPOSE: To determine whether an increase in VO2max influences plasma nitrate (NO3-), nitrite (NO2-) or the plasma NO2- response to an acute NIT dose.METHODS: Twenty seven untrained males were assessed for baseline measures of plasma NO3- and NO2- and for determination of VO2max. In a separate trial, the change in plasma NO2- following ingestion of two NIT gels (?8.1 mmol NIT) was measured. Participants were subsequently assigned to either 3-weeks sprint interval training (SIT; n=19) three times per week or a control condition (CON; n=8). SIT consisted of 4-6 repeated 15 s all out sprints on a cycle ergometer, interspersed with 4 min active recovery. The CON group were asked to maintain their normal lifestyle throughout the 3-weeks training. Participants then repeated the same experimental trials as at baseline. Differences between groups, time point and their interaction were established by two-way repeated measures ANOVA.RESULTS: VO2max, plasma NO3- and NO2- were not different between groups at baseline (SIT: VO2max 41 ± 7 ml·kg·min-1, NO2- 182 ± 65 nM, NO3- 30 ± 16 ?M; CON: VO2max 45 ± 8 ml·kg·min-1, NO2-, 170 ± 32 nM, NO3-, 41 ± 22 ?M; all P>0.15) and plasma NO2- increased to the same extent following NIT (SIT: ?NO2- 224 ± 143 nM; CON: ?NO2- = 182 ± 146 nM; both P<0.01). Following SIT, VO2max increased by 5.4% (P=0.03) but did not change in CON (P=0.74). There were no changes in plasma NO3- and NO2- within or between groups (SIT: NO2-, 192 ± 61 nM, NO3-, 37 ± 22 ?M; CON: NO2-, 200 ± 75 nM, NO3-, 45 ± 29 ?M; all P>0.15). Ingestion of NIT increased plasma NO2- post-training, however, the change was not different from pre-training (SIT: ?NO2-, 225 ± 196 nM; CON: ?NO2-, 167 ± 102 nM; both P<0.01).CONCLUSIONS: A moderate improvement in aerobic fitness following 3-weeks SIT did not alter baseline levels of plasma NO3- or NO2-, or the response to a NIT dose. Therefore, training status per se, may not be the primary factor underlying the lack of response to NIT in highly trained cohorts.
LanguageEnglish
Number of pages1
Publication statusPublished - 30 May 2015
EventAmerican College of Sports Medicine Annual Meeting - San Diego, United States
Duration: 27 May 201530 May 2015

Conference

ConferenceAmerican College of Sports Medicine Annual Meeting
Abbreviated titleACSM15
CountryUnited States
CitySan Diego
Period27/05/1530/05/15

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Nitrates
Nitric Oxide
Eating
Nitrites
Dietary Supplements
Athletes
Life Style
Analysis of Variance
Gels
Exercise

Keywords

  • dietary nitrate
  • nitric oxide
  • VO2max

Cite this

Easton, C., Muggeridge, D., Willis, G., & James, P. E. (2015). Increased VO2max does not influence nitric oxide availability or the response to a nitrate dose. Abstract from American College of Sports Medicine Annual Meeting, San Diego, United States.
Easton, Chris ; Muggeridge, David ; Willis, Gareth ; James, Philip E. / Increased VO2max does not influence nitric oxide availability or the response to a nitrate dose. Abstract from American College of Sports Medicine Annual Meeting, San Diego, United States.1 p.
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title = "Increased VO2max does not influence nitric oxide availability or the response to a nitrate dose",
abstract = "Dietary nitrate (NIT) supplementation increases nitric oxide (NO) metabolites and may improve exercise performance. However, NIT supplementation seems to be less efficacious in highly trained athletes. Despite this, no study has directly investigated the effects of a change in aerobic capacity (VO2max) on baseline plasma NO metabolites or the response to a NIT dose within the same cohort.PURPOSE: To determine whether an increase in VO2max influences plasma nitrate (NO3-), nitrite (NO2-) or the plasma NO2- response to an acute NIT dose.METHODS: Twenty seven untrained males were assessed for baseline measures of plasma NO3- and NO2- and for determination of VO2max. In a separate trial, the change in plasma NO2- following ingestion of two NIT gels (?8.1 mmol NIT) was measured. Participants were subsequently assigned to either 3-weeks sprint interval training (SIT; n=19) three times per week or a control condition (CON; n=8). SIT consisted of 4-6 repeated 15 s all out sprints on a cycle ergometer, interspersed with 4 min active recovery. The CON group were asked to maintain their normal lifestyle throughout the 3-weeks training. Participants then repeated the same experimental trials as at baseline. Differences between groups, time point and their interaction were established by two-way repeated measures ANOVA.RESULTS: VO2max, plasma NO3- and NO2- were not different between groups at baseline (SIT: VO2max 41 ± 7 ml·kg·min-1, NO2- 182 ± 65 nM, NO3- 30 ± 16 ?M; CON: VO2max 45 ± 8 ml·kg·min-1, NO2-, 170 ± 32 nM, NO3-, 41 ± 22 ?M; all P>0.15) and plasma NO2- increased to the same extent following NIT (SIT: ?NO2- 224 ± 143 nM; CON: ?NO2- = 182 ± 146 nM; both P<0.01). Following SIT, VO2max increased by 5.4{\%} (P=0.03) but did not change in CON (P=0.74). There were no changes in plasma NO3- and NO2- within or between groups (SIT: NO2-, 192 ± 61 nM, NO3-, 37 ± 22 ?M; CON: NO2-, 200 ± 75 nM, NO3-, 45 ± 29 ?M; all P>0.15). Ingestion of NIT increased plasma NO2- post-training, however, the change was not different from pre-training (SIT: ?NO2-, 225 ± 196 nM; CON: ?NO2-, 167 ± 102 nM; both P<0.01).CONCLUSIONS: A moderate improvement in aerobic fitness following 3-weeks SIT did not alter baseline levels of plasma NO3- or NO2-, or the response to a NIT dose. Therefore, training status per se, may not be the primary factor underlying the lack of response to NIT in highly trained cohorts.",
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Easton, C, Muggeridge, D, Willis, G & James, PE 2015, 'Increased VO2max does not influence nitric oxide availability or the response to a nitrate dose' American College of Sports Medicine Annual Meeting, San Diego, United States, 27/05/15 - 30/05/15, .

Increased VO2max does not influence nitric oxide availability or the response to a nitrate dose. / Easton, Chris; Muggeridge, David; Willis, Gareth; James, Philip E.

2015. Abstract from American College of Sports Medicine Annual Meeting, San Diego, United States.

Research output: Contribution to conferenceAbstract

TY - CONF

T1 - Increased VO2max does not influence nitric oxide availability or the response to a nitrate dose

AU - Easton, Chris

AU - Muggeridge, David

AU - Willis, Gareth

AU - James, Philip E.

PY - 2015/5/30

Y1 - 2015/5/30

N2 - Dietary nitrate (NIT) supplementation increases nitric oxide (NO) metabolites and may improve exercise performance. However, NIT supplementation seems to be less efficacious in highly trained athletes. Despite this, no study has directly investigated the effects of a change in aerobic capacity (VO2max) on baseline plasma NO metabolites or the response to a NIT dose within the same cohort.PURPOSE: To determine whether an increase in VO2max influences plasma nitrate (NO3-), nitrite (NO2-) or the plasma NO2- response to an acute NIT dose.METHODS: Twenty seven untrained males were assessed for baseline measures of plasma NO3- and NO2- and for determination of VO2max. In a separate trial, the change in plasma NO2- following ingestion of two NIT gels (?8.1 mmol NIT) was measured. Participants were subsequently assigned to either 3-weeks sprint interval training (SIT; n=19) three times per week or a control condition (CON; n=8). SIT consisted of 4-6 repeated 15 s all out sprints on a cycle ergometer, interspersed with 4 min active recovery. The CON group were asked to maintain their normal lifestyle throughout the 3-weeks training. Participants then repeated the same experimental trials as at baseline. Differences between groups, time point and their interaction were established by two-way repeated measures ANOVA.RESULTS: VO2max, plasma NO3- and NO2- were not different between groups at baseline (SIT: VO2max 41 ± 7 ml·kg·min-1, NO2- 182 ± 65 nM, NO3- 30 ± 16 ?M; CON: VO2max 45 ± 8 ml·kg·min-1, NO2-, 170 ± 32 nM, NO3-, 41 ± 22 ?M; all P>0.15) and plasma NO2- increased to the same extent following NIT (SIT: ?NO2- 224 ± 143 nM; CON: ?NO2- = 182 ± 146 nM; both P<0.01). Following SIT, VO2max increased by 5.4% (P=0.03) but did not change in CON (P=0.74). There were no changes in plasma NO3- and NO2- within or between groups (SIT: NO2-, 192 ± 61 nM, NO3-, 37 ± 22 ?M; CON: NO2-, 200 ± 75 nM, NO3-, 45 ± 29 ?M; all P>0.15). Ingestion of NIT increased plasma NO2- post-training, however, the change was not different from pre-training (SIT: ?NO2-, 225 ± 196 nM; CON: ?NO2-, 167 ± 102 nM; both P<0.01).CONCLUSIONS: A moderate improvement in aerobic fitness following 3-weeks SIT did not alter baseline levels of plasma NO3- or NO2-, or the response to a NIT dose. Therefore, training status per se, may not be the primary factor underlying the lack of response to NIT in highly trained cohorts.

AB - Dietary nitrate (NIT) supplementation increases nitric oxide (NO) metabolites and may improve exercise performance. However, NIT supplementation seems to be less efficacious in highly trained athletes. Despite this, no study has directly investigated the effects of a change in aerobic capacity (VO2max) on baseline plasma NO metabolites or the response to a NIT dose within the same cohort.PURPOSE: To determine whether an increase in VO2max influences plasma nitrate (NO3-), nitrite (NO2-) or the plasma NO2- response to an acute NIT dose.METHODS: Twenty seven untrained males were assessed for baseline measures of plasma NO3- and NO2- and for determination of VO2max. In a separate trial, the change in plasma NO2- following ingestion of two NIT gels (?8.1 mmol NIT) was measured. Participants were subsequently assigned to either 3-weeks sprint interval training (SIT; n=19) three times per week or a control condition (CON; n=8). SIT consisted of 4-6 repeated 15 s all out sprints on a cycle ergometer, interspersed with 4 min active recovery. The CON group were asked to maintain their normal lifestyle throughout the 3-weeks training. Participants then repeated the same experimental trials as at baseline. Differences between groups, time point and their interaction were established by two-way repeated measures ANOVA.RESULTS: VO2max, plasma NO3- and NO2- were not different between groups at baseline (SIT: VO2max 41 ± 7 ml·kg·min-1, NO2- 182 ± 65 nM, NO3- 30 ± 16 ?M; CON: VO2max 45 ± 8 ml·kg·min-1, NO2-, 170 ± 32 nM, NO3-, 41 ± 22 ?M; all P>0.15) and plasma NO2- increased to the same extent following NIT (SIT: ?NO2- 224 ± 143 nM; CON: ?NO2- = 182 ± 146 nM; both P<0.01). Following SIT, VO2max increased by 5.4% (P=0.03) but did not change in CON (P=0.74). There were no changes in plasma NO3- and NO2- within or between groups (SIT: NO2-, 192 ± 61 nM, NO3-, 37 ± 22 ?M; CON: NO2-, 200 ± 75 nM, NO3-, 45 ± 29 ?M; all P>0.15). Ingestion of NIT increased plasma NO2- post-training, however, the change was not different from pre-training (SIT: ?NO2-, 225 ± 196 nM; CON: ?NO2-, 167 ± 102 nM; both P<0.01).CONCLUSIONS: A moderate improvement in aerobic fitness following 3-weeks SIT did not alter baseline levels of plasma NO3- or NO2-, or the response to a NIT dose. Therefore, training status per se, may not be the primary factor underlying the lack of response to NIT in highly trained cohorts.

KW - dietary nitrate

KW - nitric oxide

KW - VO2max

UR - http://www.acsmannualmeeting.org/past-meetings/2015-san-diego/

M3 - Abstract

ER -

Easton C, Muggeridge D, Willis G, James PE. Increased VO2max does not influence nitric oxide availability or the response to a nitrate dose. 2015. Abstract from American College of Sports Medicine Annual Meeting, San Diego, United States.