Projects per year
Abstract
BACKGROUND: Endothelial cell TRPV4 (transient receptor potential vanilloid 4) channels provide a control point that is pivotal in regulating blood vessel diameter by mediating the Ca 2+ -dependent release of endothelial-derived vasoactive factors. In hypertension, TRPV4-mediated control of vascular function is disrupted, but the underlying mechanisms and precise physiological consequences remain controversial. METHODS: Here, using a comprehensive array of methodologies, endothelial TRPV4 channel function was examined in intact mesenteric resistance arteries from normotensive Wistar-Kyoto and spontaneously hypertensive rats. RESULTS: Our results show there is a notable shift in vascular reactivity in hypertension characterized by enhanced endothelium-dependent vasodilation at low levels of TRPV4 channel activation. However, at higher levels of TRPV4 activity, this vasodilatory response is reversed, contributing to the aberrant vascular tone observed in hypertension. The change in response, from dilation to constriction, was accompanied by a shift in intracellular Ca 2+ signaling modalities arising from TRPV4 activity. Oscillatory TRPV4-evoked IP 3 (inositol triphosphate)-mediated Ca 2+ release, which underlies dilation, decreased, while the contraction inducing sustained Ca 2+ rise, arising from TRPV4-mediated Ca 2+ influx, increased. Our findings also reveal that while the sensitivity of endothelial cell TRPV4 to activation was unchanged, expression of the channel is upregulated and IP 3 receptors are downregulated in hypertension. CONCLUSIONS: These data highlight the intricate interplay between endothelial TRPV4 channel expression, intracellular Ca 2+ signaling dynamics, and vascular reactivity. Moreover, the data support a new unifying hypothesis for the vascular impairment that accompanies hypertension. Specifically, endothelial cell TRPV4 channels play a dual role in modulating blood vessel function in hypertension.
Original language | English |
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Pages (from-to) | 57-68 |
Number of pages | 12 |
Journal | Hypertension |
Volume | 82 |
Issue number | 1 |
Early online date | 23 Oct 2024 |
DOIs | |
Publication status | Published - 1 Jan 2025 |
Keywords
- hypertension
- endothelial cell
- TRPV4
- vascular reactivity
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A multicellular endothelial signalling web coordinates vascular function and is impaired in hypertension
McCarron, J. (Principal Investigator), Buckley, C. (Co-investigator) & Wilson, C. (Co-investigator)
19/01/21 → 18/01/26
Project: Research
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Mitochondrial amplification of local calcium entry and vascular contraction in hypertension
McCarron, J. (Principal Investigator), Buckley, C. (Co-investigator), Chalmers, S. (Co-investigator) & Wilson, C. (Co-investigator)
3/04/20 → 8/01/24
Project: Research