Increased signals from short-wavelength-excited fluorescent molecules using sub-Ti:Sapphire wavelengths

G Norris, R Amor, J Dempster, W B Amos, G McConnell

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We report the use of an all-solid-state ultrashort pulsed source specifically for two-photon microscopy at wavelengths shorter than those of the conventional Ti:Sapphire laser. Our approach involves sum-frequency mixing of the output from an optical parametric oscillator (λ= 1400-1640 nm) synchronously pumped by a Yb-doped fibre laser (λ= 1064 nm), with the residual pump radiation. This generated an fs-pulsed output tunable in the red spectral region (λ= 620-636 nm, ~150 mW, 405 fs, 80 MHz, M(2) ~ 1.3). We demonstrate the performance of our ultrashort pulsed system using fluorescently labelled and autofluorescent tissue, and compare with conventional Ti:Sapphire excitation. We observe a more than 3-fold increase in fluorescence signal intensity using our visible laser source in comparison with the Ti:Sapphire laser for two-photon excitation at equal illumination peak powers of 1.16 kW or less.

LanguageEnglish
Pages200-207
Number of pages8
JournalJournal of Microscopy
Volume248
Issue number2
DOIs
Publication statusPublished - Nov 2012

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Aluminum Oxide
Lasers
Photons
Lighting
Microscopy
Fluorescence
Radiation

Keywords

  • animals
  • hosta
  • kidney
  • mice
  • microscopy, fluorescence, multiphoton
  • plant epidermis
  • plant leaves

Cite this

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title = "Increased signals from short-wavelength-excited fluorescent molecules using sub-Ti:Sapphire wavelengths",
abstract = "We report the use of an all-solid-state ultrashort pulsed source specifically for two-photon microscopy at wavelengths shorter than those of the conventional Ti:Sapphire laser. Our approach involves sum-frequency mixing of the output from an optical parametric oscillator (λ= 1400-1640 nm) synchronously pumped by a Yb-doped fibre laser (λ= 1064 nm), with the residual pump radiation. This generated an fs-pulsed output tunable in the red spectral region (λ= 620-636 nm, ~150 mW, 405 fs, 80 MHz, M(2) ~ 1.3). We demonstrate the performance of our ultrashort pulsed system using fluorescently labelled and autofluorescent tissue, and compare with conventional Ti:Sapphire excitation. We observe a more than 3-fold increase in fluorescence signal intensity using our visible laser source in comparison with the Ti:Sapphire laser for two-photon excitation at equal illumination peak powers of 1.16 kW or less.",
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author = "G Norris and R Amor and J Dempster and Amos, {W B} and G McConnell",
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Increased signals from short-wavelength-excited fluorescent molecules using sub-Ti:Sapphire wavelengths. / Norris, G; Amor, R; Dempster, J; Amos, W B; McConnell, G.

In: Journal of Microscopy, Vol. 248, No. 2, 11.2012, p. 200-207.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Increased signals from short-wavelength-excited fluorescent molecules using sub-Ti:Sapphire wavelengths

AU - Norris, G

AU - Amor, R

AU - Dempster, J

AU - Amos, W B

AU - McConnell, G

PY - 2012/11

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N2 - We report the use of an all-solid-state ultrashort pulsed source specifically for two-photon microscopy at wavelengths shorter than those of the conventional Ti:Sapphire laser. Our approach involves sum-frequency mixing of the output from an optical parametric oscillator (λ= 1400-1640 nm) synchronously pumped by a Yb-doped fibre laser (λ= 1064 nm), with the residual pump radiation. This generated an fs-pulsed output tunable in the red spectral region (λ= 620-636 nm, ~150 mW, 405 fs, 80 MHz, M(2) ~ 1.3). We demonstrate the performance of our ultrashort pulsed system using fluorescently labelled and autofluorescent tissue, and compare with conventional Ti:Sapphire excitation. We observe a more than 3-fold increase in fluorescence signal intensity using our visible laser source in comparison with the Ti:Sapphire laser for two-photon excitation at equal illumination peak powers of 1.16 kW or less.

AB - We report the use of an all-solid-state ultrashort pulsed source specifically for two-photon microscopy at wavelengths shorter than those of the conventional Ti:Sapphire laser. Our approach involves sum-frequency mixing of the output from an optical parametric oscillator (λ= 1400-1640 nm) synchronously pumped by a Yb-doped fibre laser (λ= 1064 nm), with the residual pump radiation. This generated an fs-pulsed output tunable in the red spectral region (λ= 620-636 nm, ~150 mW, 405 fs, 80 MHz, M(2) ~ 1.3). We demonstrate the performance of our ultrashort pulsed system using fluorescently labelled and autofluorescent tissue, and compare with conventional Ti:Sapphire excitation. We observe a more than 3-fold increase in fluorescence signal intensity using our visible laser source in comparison with the Ti:Sapphire laser for two-photon excitation at equal illumination peak powers of 1.16 kW or less.

KW - animals

KW - hosta

KW - kidney

KW - mice

KW - microscopy, fluorescence, multiphoton

KW - plant epidermis

KW - plant leaves

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