Increased potential of a cationic liposome-based delivery system: enhancing stability and sustained immunological activity in pre-clinical development

Afzal R Mohammed, Vincent W Bramwell, Daniel J Kirby, Sarah E McNeil, Yvonne Perrie

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

The combination of dimethyl dioctadecyl ammonium bromide (DDA) and the synthetic cord factor trehalose dibehenate (TDB) with Ag85B-ESAT-6 (H1 fusion protein) has been found to promote strong protective immune responses against Mycobacterium tuberculosis. The development of a vaccine formulation that is able to facilitate the requirements of sterility, stability and generation of a vaccine product with acceptable composition, shelf-life and safety profile may necessitate selected alterations in vaccine formulation. This study describes the implementation of a sterilisation protocol and the use of selected lyoprotective agents in order to fulfil these requirements. Concomitantly, close analysis of any alteration in physico-chemical characteristics and parameters of immunogenicity have been examined for this promising DDA liposome-based tuberculosis vaccine. The study addresses the extensive guidelines on parameters for non-clinical assessment, suitable for liposomal vaccines and other vaccine delivery systems issued by the World Health Organisation (WHO) and the European Medicines Agency (EMEA). Physical and chemical stability was observed following alteration in formulations to include novel cryoprotectants and radiation sterilisation. Immunogenicity was maintained following these alterations and even improved by modification with lysine as the cryoprotective agent for sterilised formulations. Taken together, these results outline the successful alteration to a liposomal vaccine, representing improved formulations by rational modification, whilst maintaining biological activity.

Original languageEnglish
Pages (from-to)404-412
Number of pages9
JournalEuropean Journal of Pharmaceutics and Biopharmaceutics
Volume76
Issue number3
DOIs
Publication statusPublished - 30 Nov 2010

Keywords

  • acyltransferases
  • adjuvants, immunologic
  • animals
  • bacterial antibodies
  • bacterial antigens
  • bacterial proteins
  • cations
  • drug evaluation, preclinical
  • liposomes
  • mice
  • mice, inbred BALB C
  • mycobacterium tuberculosis
  • tuberculosis vaccines

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