Increased expression of IL-16 in the brain of experimental autoimmune encephalomyelitis

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Abstract

Multiple Sclerosis (MS) is a demyelinating disease of the CNS, whose pathophysiology involves both inflammatory and neurodegenerative components. CD4+ T cells are one of the key mediators of disease initiation and progression; however CD4 i s also the receptor for the pro-inflammatory cytokine, interleukin - 16 (IL - 16). IL - 16 has been proposed to play a role in several autoimmune diseases, but the exact role of IL - 16 in the CNS during MS initiation and progression remains unclear. Therefore, the aim of this study was to examine the expression and distribution of IL - 16 in CNS tissue and investigate whether expression levels correlate with neuro-inflammation in experimental autoimmune encephalomyelitis (EAE), a murine model of MS.

EAE was induced in 6 week old C 57BL/6J female mice by immunisation with MOG35 - 55 peptide and adjuvants. Tissue was harvested at onset (day 11), peak (day 16) and resolution (day 26), and immunofluorescence staining carried out to determine CD45, CD4 and IL - 16 expression and localisation in the brain of both control and EAE mice. In addition, co-localisation of IL - 16 with CNS and immune cell subtypes was performed using a Mesolens microscope (McConnell et al., 2016), which allows subcellular detail to be obtained from wide - field epifluorescence images.

Expression of IL - 16 and CD4 was observed primarily within the lesions of cerebellum and hippocampus of the EAE brain, whereas little expression was observed in control brains. IL - 16 expression was highest at onset with 76 ±2.8% of cells ( n=3) within these lesions expressing IL - 16. This was reduced to 48±2.4% (n=3) at peak and 16 ±1.3% at resolution (n=3). Co-localization studies revealed that IL - 16 was expressed primarily by infiltrating immune cells but not by neurons or astrocytes. Co-localization of IL - 16 with immune cells in brain lesions of EAE mice suggests that infiltrating immune cells are the primary source of IL - 16. Further investigation is required if IL - 16 is pro-inflammatory or anti-inflammatory in the CNS during EAE.

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Interleukin-16
Autoimmune Experimental Encephalomyelitis
Brain
Multiple Sclerosis
Demyelinating Diseases

Keywords

  • demyelinating disease
  • pro-inflammatory cytokine
  • immune cells

Cite this

@article{e8cc87364cbd4b1cbac99ea3e9487382,
title = "Increased expression of IL-16 in the brain of experimental autoimmune encephalomyelitis",
abstract = "Multiple Sclerosis (MS) is a demyelinating disease of the CNS, whose pathophysiology involves both inflammatory and neurodegenerative components. CD4+ T cells are one of the key mediators of disease initiation and progression; however CD4 i s also the receptor for the pro-inflammatory cytokine, interleukin - 16 (IL - 16). IL - 16 has been proposed to play a role in several autoimmune diseases, but the exact role of IL - 16 in the CNS during MS initiation and progression remains unclear. Therefore, the aim of this study was to examine the expression and distribution of IL - 16 in CNS tissue and investigate whether expression levels correlate with neuro-inflammation in experimental autoimmune encephalomyelitis (EAE), a murine model of MS. EAE was induced in 6 week old C 57BL/6J female mice by immunisation with MOG35 - 55 peptide and adjuvants. Tissue was harvested at onset (day 11), peak (day 16) and resolution (day 26), and immunofluorescence staining carried out to determine CD45, CD4 and IL - 16 expression and localisation in the brain of both control and EAE mice. In addition, co-localisation of IL - 16 with CNS and immune cell subtypes was performed using a Mesolens microscope (McConnell et al., 2016), which allows subcellular detail to be obtained from wide - field epifluorescence images. Expression of IL - 16 and CD4 was observed primarily within the lesions of cerebellum and hippocampus of the EAE brain, whereas little expression was observed in control brains. IL - 16 expression was highest at onset with 76 ±2.8{\%} of cells ( n=3) within these lesions expressing IL - 16. This was reduced to 48±2.4{\%} (n=3) at peak and 16 ±1.3{\%} at resolution (n=3). Co-localization studies revealed that IL - 16 was expressed primarily by infiltrating immune cells but not by neurons or astrocytes. Co-localization of IL - 16 with immune cells in brain lesions of EAE mice suggests that infiltrating immune cells are the primary source of IL - 16. Further investigation is required if IL - 16 is pro-inflammatory or anti-inflammatory in the CNS during EAE.",
keywords = "demyelinating disease , pro-inflammatory cytokine, immune cells",
author = "Hridi, {Shehla Unaiza} and Lee McCann and Gail McConnell and Bushell, {Trevor J} and Hui-Rong Jiang",
year = "2017",
month = "4",
day = "10",
language = "English",
journal = "Brain and Neuroscience Advances",
issn = "2398-2128",

}

TY - JOUR

T1 - Increased expression of IL-16 in the brain of experimental autoimmune encephalomyelitis

AU - Hridi, Shehla Unaiza

AU - McCann, Lee

AU - McConnell, Gail

AU - Bushell, Trevor J

AU - Jiang, Hui-Rong

PY - 2017/4/10

Y1 - 2017/4/10

N2 - Multiple Sclerosis (MS) is a demyelinating disease of the CNS, whose pathophysiology involves both inflammatory and neurodegenerative components. CD4+ T cells are one of the key mediators of disease initiation and progression; however CD4 i s also the receptor for the pro-inflammatory cytokine, interleukin - 16 (IL - 16). IL - 16 has been proposed to play a role in several autoimmune diseases, but the exact role of IL - 16 in the CNS during MS initiation and progression remains unclear. Therefore, the aim of this study was to examine the expression and distribution of IL - 16 in CNS tissue and investigate whether expression levels correlate with neuro-inflammation in experimental autoimmune encephalomyelitis (EAE), a murine model of MS. EAE was induced in 6 week old C 57BL/6J female mice by immunisation with MOG35 - 55 peptide and adjuvants. Tissue was harvested at onset (day 11), peak (day 16) and resolution (day 26), and immunofluorescence staining carried out to determine CD45, CD4 and IL - 16 expression and localisation in the brain of both control and EAE mice. In addition, co-localisation of IL - 16 with CNS and immune cell subtypes was performed using a Mesolens microscope (McConnell et al., 2016), which allows subcellular detail to be obtained from wide - field epifluorescence images. Expression of IL - 16 and CD4 was observed primarily within the lesions of cerebellum and hippocampus of the EAE brain, whereas little expression was observed in control brains. IL - 16 expression was highest at onset with 76 ±2.8% of cells ( n=3) within these lesions expressing IL - 16. This was reduced to 48±2.4% (n=3) at peak and 16 ±1.3% at resolution (n=3). Co-localization studies revealed that IL - 16 was expressed primarily by infiltrating immune cells but not by neurons or astrocytes. Co-localization of IL - 16 with immune cells in brain lesions of EAE mice suggests that infiltrating immune cells are the primary source of IL - 16. Further investigation is required if IL - 16 is pro-inflammatory or anti-inflammatory in the CNS during EAE.

AB - Multiple Sclerosis (MS) is a demyelinating disease of the CNS, whose pathophysiology involves both inflammatory and neurodegenerative components. CD4+ T cells are one of the key mediators of disease initiation and progression; however CD4 i s also the receptor for the pro-inflammatory cytokine, interleukin - 16 (IL - 16). IL - 16 has been proposed to play a role in several autoimmune diseases, but the exact role of IL - 16 in the CNS during MS initiation and progression remains unclear. Therefore, the aim of this study was to examine the expression and distribution of IL - 16 in CNS tissue and investigate whether expression levels correlate with neuro-inflammation in experimental autoimmune encephalomyelitis (EAE), a murine model of MS. EAE was induced in 6 week old C 57BL/6J female mice by immunisation with MOG35 - 55 peptide and adjuvants. Tissue was harvested at onset (day 11), peak (day 16) and resolution (day 26), and immunofluorescence staining carried out to determine CD45, CD4 and IL - 16 expression and localisation in the brain of both control and EAE mice. In addition, co-localisation of IL - 16 with CNS and immune cell subtypes was performed using a Mesolens microscope (McConnell et al., 2016), which allows subcellular detail to be obtained from wide - field epifluorescence images. Expression of IL - 16 and CD4 was observed primarily within the lesions of cerebellum and hippocampus of the EAE brain, whereas little expression was observed in control brains. IL - 16 expression was highest at onset with 76 ±2.8% of cells ( n=3) within these lesions expressing IL - 16. This was reduced to 48±2.4% (n=3) at peak and 16 ±1.3% at resolution (n=3). Co-localization studies revealed that IL - 16 was expressed primarily by infiltrating immune cells but not by neurons or astrocytes. Co-localization of IL - 16 with immune cells in brain lesions of EAE mice suggests that infiltrating immune cells are the primary source of IL - 16. Further investigation is required if IL - 16 is pro-inflammatory or anti-inflammatory in the CNS during EAE.

KW - demyelinating disease

KW - pro-inflammatory cytokine

KW - immune cells

UR - https://www.bna.org.uk/meetings/bna2017/

M3 - Conference Contribution

JO - Brain and Neuroscience Advances

T2 - Brain and Neuroscience Advances

JF - Brain and Neuroscience Advances

SN - 2398-2128

M1 - P-M113

ER -