Incidence of cryptosporidiosis species in paediatric patients in Malawi

Tracy Morse, RAB Nichols, Anthony Grimason, Brian Campbell, K.C. Tembo, Huw Smith

Research output: Contribution to journalArticlepeer-review

100 Citations (Scopus)


We determined the incidence of cryptosporidiosis in children aged <5 years presenting with diarrhoea in an urban and rural hospital-based setting in Malawi. Stools were collected over a 22-month period during both rainy and dry seasons. A range of microscopic methods were used to determine the presence of Cryptosporidium spp. oocysts. Species determination was by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) of oocyst-extracted DNA using 18S rRNA and COWP gene loci. Cryptosporidium spp. oocysts were seen in 5.9% (50/848) of samples, of which 43 amplified by PCR–RFLP indicated the following species: C. hominis, C. parvum, C. hominis/C. parvum, C. meleagridis and C. andersoni. Seven samples could not be amplified by PCR. Wider species diversity was found in the rural setting, and may be a result of increased malnutrition and zoonotic exposure in this area. Improvements in water, sanitation, household hygiene and animal control are required to reduce the incidence of infection in this population.
Original languageEnglish
Pages (from-to)1307 - 1315
Number of pages9
JournalEpidemiology and Infection
Issue number8
Publication statusPublished - 1 Nov 2007


  • cryptosporidiosis
  • Malawi
  • paediatric patients


Dive into the research topics of 'Incidence of cryptosporidiosis species in paediatric patients in Malawi'. Together they form a unique fingerprint.

Cite this