Incidence of cryptosporidiosis species in paediatric patients in Malawi

Tracy Morse, RAB Nichols, Anthony Grimason, Brian Campbell, K.C. Tembo, Huw Smith

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

We determined the incidence of cryptosporidiosis in children aged <5 years presenting with diarrhoea in an urban and rural hospital-based setting in Malawi. Stools were collected over a 22-month period during both rainy and dry seasons. A range of microscopic methods were used to determine the presence of Cryptosporidium spp. oocysts. Species determination was by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) of oocyst-extracted DNA using 18S rRNA and COWP gene loci. Cryptosporidium spp. oocysts were seen in 5.9% (50/848) of samples, of which 43 amplified by PCR–RFLP indicated the following species: C. hominis, C. parvum, C. hominis/C. parvum, C. meleagridis and C. andersoni. Seven samples could not be amplified by PCR. Wider species diversity was found in the rural setting, and may be a result of increased malnutrition and zoonotic exposure in this area. Improvements in water, sanitation, household hygiene and animal control are required to reduce the incidence of infection in this population.
LanguageEnglish
Pages1307 - 1315
Number of pages9
JournalEpidemiology and Infection
Volume135
Issue number8
DOIs
Publication statusPublished - 1 Nov 2007

Fingerprint

Cryptosporidiosis
Malawi
Oocysts
Cryptosporidium
Pediatrics
Restriction Fragment Length Polymorphisms
Polymerase Chain Reaction
Incidence
Rural Hospitals
Sanitation
Urban Hospitals
Zoonoses
Hygiene
rRNA Genes
Malnutrition
Diarrhea
Water
DNA
Infection
Population

Keywords

  • cryptosporidiosis
  • Malawi
  • paediatric patients

Cite this

Morse, T., Nichols, RAB., Grimason, A., Campbell, B., Tembo, K. C., & Smith, H. (2007). Incidence of cryptosporidiosis species in paediatric patients in Malawi. Epidemiology and Infection, 135(8), 1307 - 1315. https://doi.org/10.1017/S0950268806007758
Morse, Tracy ; Nichols, RAB ; Grimason, Anthony ; Campbell, Brian ; Tembo, K.C. ; Smith, Huw. / Incidence of cryptosporidiosis species in paediatric patients in Malawi. In: Epidemiology and Infection. 2007 ; Vol. 135, No. 8. pp. 1307 - 1315.
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Morse, T, Nichols, RAB, Grimason, A, Campbell, B, Tembo, KC & Smith, H 2007, 'Incidence of cryptosporidiosis species in paediatric patients in Malawi' Epidemiology and Infection, vol. 135, no. 8, pp. 1307 - 1315. https://doi.org/10.1017/S0950268806007758

Incidence of cryptosporidiosis species in paediatric patients in Malawi. / Morse, Tracy; Nichols, RAB; Grimason, Anthony; Campbell, Brian; Tembo, K.C.; Smith, Huw.

In: Epidemiology and Infection, Vol. 135, No. 8, 01.11.2007, p. 1307 - 1315.

Research output: Contribution to journalArticle

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