Inactivation of mycobacterium paratuberculosis by pulsed electric fields

N.J. Rowan, S.J. MacGregor, J.G. Anderson, Douglas Cameron, O. Farish

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Abstract

The influence of treatment temperature and pulsed electric fields (PEF) on the viability of Mycobacterium paratuberculosis cells suspended in 0.1% (wt/vol) peptone water and in sterilized cow's milk was assessed by direct viable counts and by transmission electron microscopy (TEM). PEF treatment at 50°C (2,500 pulses at 30 kV/cm) reduced the level of viable M. paratuberculosis cells by approximately 5.3 and 5.9 log10 CFU/ml in 0.1% peptone water and in cow's milk, respectively, while PEF treatment of M. paratuberculosis at lower temperatures resulted in less lethality. Heating alone at 50°C for 25 min or at 72°C for 25 s (extended high-temperature, short-time pasteurization) resulted in reductions of M. paratuberculosis of approximately 0.01 and 2.4 log10 CFU/ml, respectively. TEM studies revealed that exposure to PEF treatment resulted in substantial damage at the cellular level to M. paratuberculosis.
Original languageEnglish
Pages (from-to)2833-2836
Number of pages3
JournalApplied and Environmental Microbiology
Volume67
Issue number6
DOIs
Publication statusPublished - Jun 2001

Keywords

  • pulsed electric fields
  • cells
  • mycobacterium paratuberculosis

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