Inactivation of mycobacterium paratuberculosis by pulsed electric fields

N.J. Rowan, S.J. MacGregor, J.G. Anderson, Douglas Cameron, O. Farish

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The influence of treatment temperature and pulsed electric fields (PEF) on the viability of Mycobacterium paratuberculosis cells suspended in 0.1% (wt/vol) peptone water and in sterilized cow's milk was assessed by direct viable counts and by transmission electron microscopy (TEM). PEF treatment at 50°C (2,500 pulses at 30 kV/cm) reduced the level of viable M. paratuberculosis cells by approximately 5.3 and 5.9 log10 CFU/ml in 0.1% peptone water and in cow's milk, respectively, while PEF treatment of M. paratuberculosis at lower temperatures resulted in less lethality. Heating alone at 50°C for 25 min or at 72°C for 25 s (extended high-temperature, short-time pasteurization) resulted in reductions of M. paratuberculosis of approximately 0.01 and 2.4 log10 CFU/ml, respectively. TEM studies revealed that exposure to PEF treatment resulted in substantial damage at the cellular level to M. paratuberculosis.
LanguageEnglish
Pages2833-2836
Number of pages3
JournalApplied and Environmental Microbiology
Volume67
Issue number6
DOIs
Publication statusPublished - Jun 2001

Fingerprint

Mycobacterium avium subsp. paratuberculosis
pulsed electric fields
electric field
inactivation
Peptones
milk
peptones
transmission electron microscopy
Transmission Electron Microscopy
Temperature
Milk
high-temperature short-time pasteurization
Pasteurization
Water
viability
Heating
heating
temperature
water
damage

Keywords

  • pulsed electric fields
  • cells
  • mycobacterium paratuberculosis

Cite this

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Inactivation of mycobacterium paratuberculosis by pulsed electric fields. / Rowan, N.J.; MacGregor, S.J.; Anderson, J.G.; Cameron, Douglas; Farish, O.

In: Applied and Environmental Microbiology, Vol. 67, No. 6, 06.2001, p. 2833-2836.

Research output: Contribution to journalArticle

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AU - MacGregor, S.J.

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AU - Cameron, Douglas

AU - Farish, O.

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AB - The influence of treatment temperature and pulsed electric fields (PEF) on the viability of Mycobacterium paratuberculosis cells suspended in 0.1% (wt/vol) peptone water and in sterilized cow's milk was assessed by direct viable counts and by transmission electron microscopy (TEM). PEF treatment at 50°C (2,500 pulses at 30 kV/cm) reduced the level of viable M. paratuberculosis cells by approximately 5.3 and 5.9 log10 CFU/ml in 0.1% peptone water and in cow's milk, respectively, while PEF treatment of M. paratuberculosis at lower temperatures resulted in less lethality. Heating alone at 50°C for 25 min or at 72°C for 25 s (extended high-temperature, short-time pasteurization) resulted in reductions of M. paratuberculosis of approximately 0.01 and 2.4 log10 CFU/ml, respectively. TEM studies revealed that exposure to PEF treatment resulted in substantial damage at the cellular level to M. paratuberculosis.

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