Inactivation of microorganisms within collagen gel biomatrices using pulsed electric field treatment

Sarah Griffiths, Michelle Maclean, John G. Anderson, Scott J. Macgregor, H. Mary Grant

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Pulsed electric field (PEF) treatment was examined as a potential decontamination method for tissue engineering biomatrices by determining the susceptibility of a range of microorganisms whilst within a collagen gel. High intensity pulsed electric fields were applied to collagen gel biomatrices containing either Escherichia coli, Pseudomonas aeruginosa, Staphylococcus epidermidis, Candida albicans, Saccharomyces cerevisiae or the spores of Aspergillus niger. The results established varying degrees of microbial PEF susceptibility. When high initial cell densities (106–107 CFU ml−1) were PEF treated with 100 pulses at 45 kV cm−1, the greatest log reduction was achieved with S. cerevisiae (~6.5 log10 CFU ml−1) and the lowest reduction achieved with S. epidermidis (~0.5 log10 CFU ml−1). The results demonstrate that inactivation is influenced by the intrinsic properties of the microorganism treated. Further investigations are required to optimise the microbial inactivation kinetics associated with PEF treatment of collagen gel biomatrices.
LanguageEnglish
Pages507-515
Number of pages9
JournalJournal of Materials Science: Materials in Medicine
Volume23
Issue number2
Early online date29 Dec 2011
DOIs
Publication statusPublished - Feb 2012

Fingerprint

Collagen
Microorganisms
Staphylococcus epidermidis
Gels
Electric fields
Saccharomyces cerevisiae
Microbial Viability
Decontamination
Aspergillus niger
Yeast
Tissue Engineering
Spores
Candida albicans
Pseudomonas aeruginosa
Cell Count
Candida
Escherichia coli
Aspergillus
Bioelectric potentials
Tissue engineering

Keywords

  • collagen gel
  • biomatrices
  • electric field treatment

Cite this

@article{6782114280094cd9add586181dd1ea01,
title = "Inactivation of microorganisms within collagen gel biomatrices using pulsed electric field treatment",
abstract = "Pulsed electric field (PEF) treatment was examined as a potential decontamination method for tissue engineering biomatrices by determining the susceptibility of a range of microorganisms whilst within a collagen gel. High intensity pulsed electric fields were applied to collagen gel biomatrices containing either Escherichia coli, Pseudomonas aeruginosa, Staphylococcus epidermidis, Candida albicans, Saccharomyces cerevisiae or the spores of Aspergillus niger. The results established varying degrees of microbial PEF susceptibility. When high initial cell densities (106–107 CFU ml−1) were PEF treated with 100 pulses at 45 kV cm−1, the greatest log reduction was achieved with S. cerevisiae (~6.5 log10 CFU ml−1) and the lowest reduction achieved with S. epidermidis (~0.5 log10 CFU ml−1). The results demonstrate that inactivation is influenced by the intrinsic properties of the microorganism treated. Further investigations are required to optimise the microbial inactivation kinetics associated with PEF treatment of collagen gel biomatrices.",
keywords = "collagen gel , biomatrices, electric field treatment",
author = "Sarah Griffiths and Michelle Maclean and Anderson, {John G.} and Macgregor, {Scott J.} and Grant, {H. Mary}",
year = "2012",
month = "2",
doi = "10.1007/s10856-011-4526-x",
language = "English",
volume = "23",
pages = "507--515",
journal = "Journal of Materials Science: Materials in Medicine",
issn = "0957-4530",
number = "2",

}

TY - JOUR

T1 - Inactivation of microorganisms within collagen gel biomatrices using pulsed electric field treatment

AU - Griffiths, Sarah

AU - Maclean, Michelle

AU - Anderson, John G.

AU - Macgregor, Scott J.

AU - Grant, H. Mary

PY - 2012/2

Y1 - 2012/2

N2 - Pulsed electric field (PEF) treatment was examined as a potential decontamination method for tissue engineering biomatrices by determining the susceptibility of a range of microorganisms whilst within a collagen gel. High intensity pulsed electric fields were applied to collagen gel biomatrices containing either Escherichia coli, Pseudomonas aeruginosa, Staphylococcus epidermidis, Candida albicans, Saccharomyces cerevisiae or the spores of Aspergillus niger. The results established varying degrees of microbial PEF susceptibility. When high initial cell densities (106–107 CFU ml−1) were PEF treated with 100 pulses at 45 kV cm−1, the greatest log reduction was achieved with S. cerevisiae (~6.5 log10 CFU ml−1) and the lowest reduction achieved with S. epidermidis (~0.5 log10 CFU ml−1). The results demonstrate that inactivation is influenced by the intrinsic properties of the microorganism treated. Further investigations are required to optimise the microbial inactivation kinetics associated with PEF treatment of collagen gel biomatrices.

AB - Pulsed electric field (PEF) treatment was examined as a potential decontamination method for tissue engineering biomatrices by determining the susceptibility of a range of microorganisms whilst within a collagen gel. High intensity pulsed electric fields were applied to collagen gel biomatrices containing either Escherichia coli, Pseudomonas aeruginosa, Staphylococcus epidermidis, Candida albicans, Saccharomyces cerevisiae or the spores of Aspergillus niger. The results established varying degrees of microbial PEF susceptibility. When high initial cell densities (106–107 CFU ml−1) were PEF treated with 100 pulses at 45 kV cm−1, the greatest log reduction was achieved with S. cerevisiae (~6.5 log10 CFU ml−1) and the lowest reduction achieved with S. epidermidis (~0.5 log10 CFU ml−1). The results demonstrate that inactivation is influenced by the intrinsic properties of the microorganism treated. Further investigations are required to optimise the microbial inactivation kinetics associated with PEF treatment of collagen gel biomatrices.

KW - collagen gel

KW - biomatrices

KW - electric field treatment

U2 - 10.1007/s10856-011-4526-x

DO - 10.1007/s10856-011-4526-x

M3 - Article

VL - 23

SP - 507

EP - 515

JO - Journal of Materials Science: Materials in Medicine

T2 - Journal of Materials Science: Materials in Medicine

JF - Journal of Materials Science: Materials in Medicine

SN - 0957-4530

IS - 2

ER -