Pulsed electric field (PEF) treatment was examined as a potential decontamination method for tissue engineering biomatrices by determining the susceptibility of a range of microorganisms whilst within a collagen gel. High intensity pulsed electric fields were applied to collagen gel biomatrices containing either Escherichia coli, Pseudomonas aeruginosa, Staphylococcus epidermidis, Candida albicans, Saccharomyces cerevisiae or the spores of Aspergillus niger. The results established varying degrees of microbial PEF susceptibility. When high initial cell densities (106–107 CFU ml−1) were PEF treated with 100 pulses at 45 kV cm−1, the greatest log reduction was achieved with S. cerevisiae (~6.5 log10 CFU ml−1) and the lowest reduction achieved with S. epidermidis (~0.5 log10 CFU ml−1). The results demonstrate that inactivation is influenced by the intrinsic properties of the microorganism treated. Further investigations are required to optimise the microbial inactivation kinetics associated with PEF treatment of collagen gel biomatrices.
|Number of pages||9|
|Journal||Journal of Materials Science: Materials in Medicine|
|Early online date||29 Dec 2011|
|Publication status||Published - Feb 2012|
- collagen gel
- electric field treatment