Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 283 |
| Number of pages | 288 |
| Journal | The Bone and Joint Journal |
| Volume | 97-B |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 2015 |
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Keywords
- lower limb arthroplasty
- high intensity narrow spectrum light
- bacteria
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Inactivation of micro-organisms isolated from infected lower limb arthroplasties using high-intensity narrow-spectrum (HINS) light. / Gupta, Sanjay; MacLean, Michelle; Anderson, John; MacGregor, Scott; Meek, Dominic; Grant, Mary.
In: The Bone and Joint Journal, Vol. 97-B, No. 2, 02.2015, p. 283.Research output: Contribution to journal › Article
TY - JOUR
T1 - Inactivation of micro-organisms isolated from infected lower limb arthroplasties using high-intensity narrow-spectrum (HINS) light
AU - Gupta, Sanjay
AU - MacLean, Michelle
AU - Anderson, John
AU - MacGregor, Scott
AU - Meek, Dominic
AU - Grant, Mary
PY - 2015/2
Y1 - 2015/2
N2 - High-Intensity Narrow-Spectrum (HINS) light is a novel blue light inactivation technology which kills bacteria through a photodynamic process, and has been shown to have bactericidal activity against a wide range of species. Specimens from patients with infected hip and knee arthroplasties were collected over a one year period (1 May 2009 – 30 April 2010). A range of these microbial isolates was tested for sensitivity to HINS-light. During testing, suspensions of the pathogens were exposed to increasing doses of HINS-light (of 123mW/cm2 irradiance). Non-light exposed control samples were also used. The samples were then plated onto agar plates and incubated at 37°C for 24 hours before enumeration. Complete inactivation (greater than 4-log10 reduction) was achieved for all of the isolates. The typical inactivation curve showed a slow initial reaction followed by a rapid period of inactivation. The doses of HINS-light required ranged between 118 and 2214 J/cm2. Gram-positive bacteria were generally found to be more susceptible than Gram-negative. As HINS-light uses visible wavelengths, it can be safely used in the presence of patients and staff.This unique feature could lead to its possible use in the prevention of infection during surgery and post-operative dressing changes.
AB - High-Intensity Narrow-Spectrum (HINS) light is a novel blue light inactivation technology which kills bacteria through a photodynamic process, and has been shown to have bactericidal activity against a wide range of species. Specimens from patients with infected hip and knee arthroplasties were collected over a one year period (1 May 2009 – 30 April 2010). A range of these microbial isolates was tested for sensitivity to HINS-light. During testing, suspensions of the pathogens were exposed to increasing doses of HINS-light (of 123mW/cm2 irradiance). Non-light exposed control samples were also used. The samples were then plated onto agar plates and incubated at 37°C for 24 hours before enumeration. Complete inactivation (greater than 4-log10 reduction) was achieved for all of the isolates. The typical inactivation curve showed a slow initial reaction followed by a rapid period of inactivation. The doses of HINS-light required ranged between 118 and 2214 J/cm2. Gram-positive bacteria were generally found to be more susceptible than Gram-negative. As HINS-light uses visible wavelengths, it can be safely used in the presence of patients and staff.This unique feature could lead to its possible use in the prevention of infection during surgery and post-operative dressing changes.
KW - lower limb arthroplasty
KW - high intensity narrow spectrum light
KW - bacteria
U2 - 10.1302/0301-620X.97B2.35154
DO - 10.1302/0301-620X.97B2.35154
M3 - Article
VL - 97-B
SP - 283
JO - The Bone and Joint Journal
JF - The Bone and Joint Journal
SN - 2049-4394
IS - 2
ER -