Inactivation of micro-organisms isolated from infected lower limb arthroplasties using high-intensity narrow-spectrum (HINS) light

Sanjay Gupta, Michelle MacLean, John Anderson, Scott MacGregor, Dominic Meek, Mary Grant

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

High-Intensity Narrow-Spectrum (HINS) light is a novel blue light inactivation technology which kills bacteria through a photodynamic process, and has been shown to have bactericidal activity against a wide range of species. Specimens from patients with infected hip and knee arthroplasties were collected over a one year period (1 May 2009 – 30 April 2010). A range of these microbial isolates was tested for sensitivity to HINS-light. During testing, suspensions of the pathogens were exposed to increasing doses of HINS-light (of 123mW/cm2 irradiance). Non-light exposed control samples were also used. The samples were then plated onto agar plates and incubated at 37°C for 24 hours before enumeration. Complete inactivation (greater than 4-log10 reduction) was achieved for all of the isolates. The typical inactivation curve showed a slow initial reaction followed by a rapid period of inactivation. The doses of HINS-light required ranged between 118 and 2214 J/cm2. Gram-positive bacteria were generally found to be more susceptible than Gram-negative. As HINS-light uses visible wavelengths, it can be safely used in the presence of patients and staff.This unique feature could lead to its possible use in the prevention of infection during surgery and post-operative dressing changes.
LanguageEnglish
Pages283
Number of pages288
JournalThe Bone and Joint Journal
Volume97-B
Issue number2
DOIs
Publication statusPublished - Feb 2015

Fingerprint

Arthroplasty
Lower Extremity
Light
Dosimetry
Bacteria
Knee Replacement Arthroplasties
Gram-Positive Bacteria
Pathogens
Bandages
Surgery
Agar
Hip
Suspensions
Technology
Wavelength
Testing
Infection

Keywords

  • lower limb arthroplasty
  • high intensity narrow spectrum light
  • bacteria

Cite this

@article{6f8a14130ad94f64bbee94fdb5170bdf,
title = "Inactivation of micro-organisms isolated from infected lower limb arthroplasties using high-intensity narrow-spectrum (HINS) light",
abstract = "High-Intensity Narrow-Spectrum (HINS) light is a novel blue light inactivation technology which kills bacteria through a photodynamic process, and has been shown to have bactericidal activity against a wide range of species. Specimens from patients with infected hip and knee arthroplasties were collected over a one year period (1 May 2009 – 30 April 2010). A range of these microbial isolates was tested for sensitivity to HINS-light. During testing, suspensions of the pathogens were exposed to increasing doses of HINS-light (of 123mW/cm2 irradiance). Non-light exposed control samples were also used. The samples were then plated onto agar plates and incubated at 37°C for 24 hours before enumeration. Complete inactivation (greater than 4-log10 reduction) was achieved for all of the isolates. The typical inactivation curve showed a slow initial reaction followed by a rapid period of inactivation. The doses of HINS-light required ranged between 118 and 2214 J/cm2. Gram-positive bacteria were generally found to be more susceptible than Gram-negative. As HINS-light uses visible wavelengths, it can be safely used in the presence of patients and staff.This unique feature could lead to its possible use in the prevention of infection during surgery and post-operative dressing changes.",
keywords = "lower limb arthroplasty, high intensity narrow spectrum light, bacteria",
author = "Sanjay Gupta and Michelle MacLean and John Anderson and Scott MacGregor and Dominic Meek and Mary Grant",
year = "2015",
month = "2",
doi = "10.1302/0301-620X.97B2.35154",
language = "English",
volume = "97-B",
pages = "283",
journal = "The Bone and Joint Journal",
issn = "2049-4394",
number = "2",

}

TY - JOUR

T1 - Inactivation of micro-organisms isolated from infected lower limb arthroplasties using high-intensity narrow-spectrum (HINS) light

AU - Gupta, Sanjay

AU - MacLean, Michelle

AU - Anderson, John

AU - MacGregor, Scott

AU - Meek, Dominic

AU - Grant, Mary

PY - 2015/2

Y1 - 2015/2

N2 - High-Intensity Narrow-Spectrum (HINS) light is a novel blue light inactivation technology which kills bacteria through a photodynamic process, and has been shown to have bactericidal activity against a wide range of species. Specimens from patients with infected hip and knee arthroplasties were collected over a one year period (1 May 2009 – 30 April 2010). A range of these microbial isolates was tested for sensitivity to HINS-light. During testing, suspensions of the pathogens were exposed to increasing doses of HINS-light (of 123mW/cm2 irradiance). Non-light exposed control samples were also used. The samples were then plated onto agar plates and incubated at 37°C for 24 hours before enumeration. Complete inactivation (greater than 4-log10 reduction) was achieved for all of the isolates. The typical inactivation curve showed a slow initial reaction followed by a rapid period of inactivation. The doses of HINS-light required ranged between 118 and 2214 J/cm2. Gram-positive bacteria were generally found to be more susceptible than Gram-negative. As HINS-light uses visible wavelengths, it can be safely used in the presence of patients and staff.This unique feature could lead to its possible use in the prevention of infection during surgery and post-operative dressing changes.

AB - High-Intensity Narrow-Spectrum (HINS) light is a novel blue light inactivation technology which kills bacteria through a photodynamic process, and has been shown to have bactericidal activity against a wide range of species. Specimens from patients with infected hip and knee arthroplasties were collected over a one year period (1 May 2009 – 30 April 2010). A range of these microbial isolates was tested for sensitivity to HINS-light. During testing, suspensions of the pathogens were exposed to increasing doses of HINS-light (of 123mW/cm2 irradiance). Non-light exposed control samples were also used. The samples were then plated onto agar plates and incubated at 37°C for 24 hours before enumeration. Complete inactivation (greater than 4-log10 reduction) was achieved for all of the isolates. The typical inactivation curve showed a slow initial reaction followed by a rapid period of inactivation. The doses of HINS-light required ranged between 118 and 2214 J/cm2. Gram-positive bacteria were generally found to be more susceptible than Gram-negative. As HINS-light uses visible wavelengths, it can be safely used in the presence of patients and staff.This unique feature could lead to its possible use in the prevention of infection during surgery and post-operative dressing changes.

KW - lower limb arthroplasty

KW - high intensity narrow spectrum light

KW - bacteria

U2 - 10.1302/0301-620X.97B2.35154

DO - 10.1302/0301-620X.97B2.35154

M3 - Article

VL - 97-B

SP - 283

JO - The Bone and Joint Journal

T2 - The Bone and Joint Journal

JF - The Bone and Joint Journal

SN - 2049-4394

IS - 2

ER -