Inactivation of bacterial pathogens following exposure to light from a 405-nanometer light-emitting diode array

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Abstract

This study demonstrates the susceptibility of a variety of medically important bacteria to inactivation by 405-nm light from an array of light-emitting diodes (LEDs), without the application of exogenous photosensitizer molecules. Selected bacterial pathogens, all commonly associated with hospital-acquired infections, were exposed to the 405-nm LED array, and the results show that both gram-positive and gram-negative species were successfully inactivated, with the general trend showing gram-positive species to be more susceptible than gram-negative bacteria. Detailed investigation of the bactericidal effect of the blue-light treatment on Staphylococcus aureus suspensions, for a range of different population densities, demonstrated that 405-nm LED array illumination can cause complete inactivation at high population densities: inactivation levels corresponding to a 9-log(10) reduction were achieved. The results, which show the inactivation of a wide range of medically important bacteria including methicillin-resistant Staphylococcus aureus, demonstrate that, with further development, narrow-spectrum 405-nm visible-light illumination from an LED source has the potential to provide a novel decontamination method with a wide range of potential applications.
Original languageEnglish
Pages (from-to)1932-1937
Number of pages6
JournalApplied and Environmental Microbiology
Volume75
Issue number7
Early online date6 Feb 2009
DOIs
Publication statusPublished - Apr 2009

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Pathogens
Light emitting diodes
inactivation
pathogen
Light
Bacteria
pathogens
Lighting
Photosensitizers
Population Density
lighting
Decontamination
population density
bacterium
Light sources
cross infection
antibacterial properties
bacteria
decontamination
blue light

Keywords

  • delta aminolevulinic acid
  • photodynamic therapy
  • blue light
  • in vitro
  • staphylococcus aureus
  • escherichia-coli
  • visible light
  • porphyrin
  • spectrum

Cite this

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title = "Inactivation of bacterial pathogens following exposure to light from a 405-nanometer light-emitting diode array",
abstract = "This study demonstrates the susceptibility of a variety of medically important bacteria to inactivation by 405-nm light from an array of light-emitting diodes (LEDs), without the application of exogenous photosensitizer molecules. Selected bacterial pathogens, all commonly associated with hospital-acquired infections, were exposed to the 405-nm LED array, and the results show that both gram-positive and gram-negative species were successfully inactivated, with the general trend showing gram-positive species to be more susceptible than gram-negative bacteria. Detailed investigation of the bactericidal effect of the blue-light treatment on Staphylococcus aureus suspensions, for a range of different population densities, demonstrated that 405-nm LED array illumination can cause complete inactivation at high population densities: inactivation levels corresponding to a 9-log(10) reduction were achieved. The results, which show the inactivation of a wide range of medically important bacteria including methicillin-resistant Staphylococcus aureus, demonstrate that, with further development, narrow-spectrum 405-nm visible-light illumination from an LED source has the potential to provide a novel decontamination method with a wide range of potential applications.",
keywords = "delta aminolevulinic acid, photodynamic therapy, blue light, in vitro, staphylococcus aureus, escherichia-coli, visible light, porphyrin, spectrum",
author = "Michelle Maclean and S.J. MacGregor and J.G. Anderson and G. Woolsey",
year = "2009",
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T1 - Inactivation of bacterial pathogens following exposure to light from a 405-nanometer light-emitting diode array

AU - Maclean, Michelle

AU - MacGregor, S.J.

AU - Anderson, J.G.

AU - Woolsey, G.

PY - 2009/4

Y1 - 2009/4

N2 - This study demonstrates the susceptibility of a variety of medically important bacteria to inactivation by 405-nm light from an array of light-emitting diodes (LEDs), without the application of exogenous photosensitizer molecules. Selected bacterial pathogens, all commonly associated with hospital-acquired infections, were exposed to the 405-nm LED array, and the results show that both gram-positive and gram-negative species were successfully inactivated, with the general trend showing gram-positive species to be more susceptible than gram-negative bacteria. Detailed investigation of the bactericidal effect of the blue-light treatment on Staphylococcus aureus suspensions, for a range of different population densities, demonstrated that 405-nm LED array illumination can cause complete inactivation at high population densities: inactivation levels corresponding to a 9-log(10) reduction were achieved. The results, which show the inactivation of a wide range of medically important bacteria including methicillin-resistant Staphylococcus aureus, demonstrate that, with further development, narrow-spectrum 405-nm visible-light illumination from an LED source has the potential to provide a novel decontamination method with a wide range of potential applications.

AB - This study demonstrates the susceptibility of a variety of medically important bacteria to inactivation by 405-nm light from an array of light-emitting diodes (LEDs), without the application of exogenous photosensitizer molecules. Selected bacterial pathogens, all commonly associated with hospital-acquired infections, were exposed to the 405-nm LED array, and the results show that both gram-positive and gram-negative species were successfully inactivated, with the general trend showing gram-positive species to be more susceptible than gram-negative bacteria. Detailed investigation of the bactericidal effect of the blue-light treatment on Staphylococcus aureus suspensions, for a range of different population densities, demonstrated that 405-nm LED array illumination can cause complete inactivation at high population densities: inactivation levels corresponding to a 9-log(10) reduction were achieved. The results, which show the inactivation of a wide range of medically important bacteria including methicillin-resistant Staphylococcus aureus, demonstrate that, with further development, narrow-spectrum 405-nm visible-light illumination from an LED source has the potential to provide a novel decontamination method with a wide range of potential applications.

KW - delta aminolevulinic acid

KW - photodynamic therapy

KW - blue light

KW - in vitro

KW - staphylococcus aureus

KW - escherichia-coli

KW - visible light

KW - porphyrin

KW - spectrum

U2 - 10.1128/AEM.01892-08

DO - 10.1128/AEM.01892-08

M3 - Article

VL - 75

SP - 1932

EP - 1937

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 7

ER -