Inactivation of bacterial pathogens following exposure to light from a 405-nanometer light-emitting diode array

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Abstract

This study demonstrates the susceptibility of a variety of medically important bacteria to inactivation by 405-nm light from an array of light-emitting diodes (LEDs), without the application of exogenous photosensitizer molecules. Selected bacterial pathogens, all commonly associated with hospital-acquired infections, were exposed to the 405-nm LED array, and the results show that both gram-positive and gram-negative species were successfully inactivated, with the general trend showing gram-positive species to be more susceptible than gram-negative bacteria. Detailed investigation of the bactericidal effect of the blue-light treatment on Staphylococcus aureus suspensions, for a range of different population densities, demonstrated that 405-nm LED array illumination can cause complete inactivation at high population densities: inactivation levels corresponding to a 9-log(10) reduction were achieved. The results, which show the inactivation of a wide range of medically important bacteria including methicillin-resistant Staphylococcus aureus, demonstrate that, with further development, narrow-spectrum 405-nm visible-light illumination from an LED source has the potential to provide a novel decontamination method with a wide range of potential applications.
Original languageEnglish
Pages (from-to)1932-1937
Number of pages6
JournalApplied and Environmental Microbiology
Volume75
Issue number7
Early online date6 Feb 2009
DOIs
Publication statusPublished - Apr 2009

Keywords

  • delta aminolevulinic acid
  • photodynamic therapy
  • blue light
  • in vitro
  • staphylococcus aureus
  • escherichia-coli
  • visible light
  • porphyrin
  • spectrum

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