Inactivating alternative NADH dehydrogenases

enhancing fungal bioprocesses by improving growth and biomass yield?

Ioannis Voulgaris, Andrew O'Donnell, Linda Harvey, Brian McNeil

Research output: Contribution to journalArticle

11 Citations (Scopus)
9 Downloads (Pure)

Abstract

Debate still surrounds the physiological roles of the alternative respiratory enzymes found in many fungi and plants. It has been proposed that alternative NADH:ubiquinone oxidoreductases (NADH dehydrogenases) may protect against oxidative stress, conversely, elevated activity of these enzymes has been linked to senescence. Here we show that inhibition of these enzymes in a fungal protein expression system (Aspergillus niger) leads to significantly enhanced specific growth rate, substrate uptake, carbon dioxide evolution, higher protein content, and more efficient use of substrates. These findings are consistent with a protective role of the NADH dehydrogenases against oxidative stress, thus, when electron flow via these enzymes is blocked, flux through the main respiratory pathway rises, leading to enhanced ATP generation. We anticipate that our findings will stimulate further studies in fungal and plant cultures leading to significant improvements in these expression systems, and to deeper insights into the cellular roles of alternative respiration.

Original languageEnglish
Article number322
Number of pages9
JournalScientific Reports
Volume2
DOIs
Publication statusPublished - 20 Mar 2012

Fingerprint

NADH Dehydrogenase
Biomass
Enzymes
Growth
Oxidative Stress
Electron Transport Complex I
Fungal Proteins
Aspergillus niger
Carbon Dioxide
Respiration
Fungi
Adenosine Triphosphate
Electrons
Proteins

Keywords

  • adenosine triphosphate
  • biomass
  • carbon dioxide
  • fungi
  • glucose
  • NADH dehydrogenases
  • quaternary ammonium compounds
  • superoxides

Cite this

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title = "Inactivating alternative NADH dehydrogenases: enhancing fungal bioprocesses by improving growth and biomass yield?",
abstract = "Debate still surrounds the physiological roles of the alternative respiratory enzymes found in many fungi and plants. It has been proposed that alternative NADH:ubiquinone oxidoreductases (NADH dehydrogenases) may protect against oxidative stress, conversely, elevated activity of these enzymes has been linked to senescence. Here we show that inhibition of these enzymes in a fungal protein expression system (Aspergillus niger) leads to significantly enhanced specific growth rate, substrate uptake, carbon dioxide evolution, higher protein content, and more efficient use of substrates. These findings are consistent with a protective role of the NADH dehydrogenases against oxidative stress, thus, when electron flow via these enzymes is blocked, flux through the main respiratory pathway rises, leading to enhanced ATP generation. We anticipate that our findings will stimulate further studies in fungal and plant cultures leading to significant improvements in these expression systems, and to deeper insights into the cellular roles of alternative respiration.",
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Inactivating alternative NADH dehydrogenases : enhancing fungal bioprocesses by improving growth and biomass yield? / Voulgaris, Ioannis; O'Donnell, Andrew; Harvey, Linda; McNeil, Brian.

In: Scientific Reports, Vol. 2, 322, 20.03.2012.

Research output: Contribution to journalArticle

TY - JOUR

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T2 - enhancing fungal bioprocesses by improving growth and biomass yield?

AU - Voulgaris, Ioannis

AU - O'Donnell, Andrew

AU - Harvey, Linda

AU - McNeil, Brian

PY - 2012/3/20

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AB - Debate still surrounds the physiological roles of the alternative respiratory enzymes found in many fungi and plants. It has been proposed that alternative NADH:ubiquinone oxidoreductases (NADH dehydrogenases) may protect against oxidative stress, conversely, elevated activity of these enzymes has been linked to senescence. Here we show that inhibition of these enzymes in a fungal protein expression system (Aspergillus niger) leads to significantly enhanced specific growth rate, substrate uptake, carbon dioxide evolution, higher protein content, and more efficient use of substrates. These findings are consistent with a protective role of the NADH dehydrogenases against oxidative stress, thus, when electron flow via these enzymes is blocked, flux through the main respiratory pathway rises, leading to enhanced ATP generation. We anticipate that our findings will stimulate further studies in fungal and plant cultures leading to significant improvements in these expression systems, and to deeper insights into the cellular roles of alternative respiration.

KW - adenosine triphosphate

KW - biomass

KW - carbon dioxide

KW - fungi

KW - glucose

KW - NADH dehydrogenases

KW - quaternary ammonium compounds

KW - superoxides

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