In vivo validation of a miniaturised electrochemical oxygen sensor for measuring intestinal oxygen tension

Mark E. Gray, Jamie R. K. Marland, Camelia Dunare, Ewen O. Blair, James Meehan, Andreas Tsiamis, Ian H. Kunkler, Alan F. Murray, David Argyle, Alex Dyson, Mervyn Singer, Mark A. Potter

Research output: Contribution to journalArticle

Abstract

Recent advances in the fields of electronics and microfabrication techniques have led to the development of implantable medical devices for use within the field of precision medicine. Monitoring visceral surface tissue O2 tension (ptO2) by means of an implantable sensor is potentially useful in many clinical situations including the peri-operative management of patients undergoing intestinal resection and anastomosis. This concept could provide a means by which treatment could be tailored to individual patients. This study describes the in vivo validation of a novel miniaturised electrochemical O2 sensor to provide real-time data on intestinal ptO2. A single O2 sensor was placed onto the serosal surface of the small intestine of anaesthetised rats that were exposed to ischaemic (superior mesenteric artery occlusion) and hypoxaemic (alterations in inspired fractional O2 concentrations) insults. Control experiments demonstrated that the sensors function and remain stable in an in vivo environment. Intestinal ptO2 decreased following superior mesenteric artery occlusion and with reductions in inspired O2 concentrations. These results were reversible after reinstating blood flow or increasing inspired O2 concentrations. We have successfully developed an anaesthetised rat intestinal ischaemic and hypoxic model for validation of a miniaturised O2 sensor to provide real-time measurement of intestinal ptO2. Our results support further validation of the sensors in physiological conditions using a large animal model to provide evidence of their use in clinical applications where monitoring visceral surface tissue O2 tension is important.
LanguageEnglish
PagesG242-G252
Number of pages11
JournalAmerican Journal of Physiology-Gastrointestinal and Liver Physiology
Volume317
Issue number2
Early online date12 Jun 2019
DOIs
Publication statusPublished - 1 Aug 2019

Fingerprint

Oxygen sensors
Electrochemical sensors
Oxygen
Sensors
Rats
Tissue
Monitoring
Microfabrication
Time measurement
Medicine
Animals
Blood
Electronic equipment
Experiments

Keywords

  • hypoxaemia
  • intestinal resection and anastomosis
  • intestinal serosal tissue oxygen tension
  • miniaturised electrochemical oxygen sensor
  • superior mesenteric artery occlusion

Cite this

Gray, Mark E. ; Marland, Jamie R. K. ; Dunare, Camelia ; Blair, Ewen O. ; Meehan, James ; Tsiamis, Andreas ; Kunkler, Ian H. ; Murray, Alan F. ; Argyle, David ; Dyson, Alex ; Singer, Mervyn ; Potter, Mark A. / In vivo validation of a miniaturised electrochemical oxygen sensor for measuring intestinal oxygen tension. In: American Journal of Physiology-Gastrointestinal and Liver Physiology. 2019 ; Vol. 317, No. 2. pp. G242-G252.
@article{8d00d74f5a224915b33a1cdef26082a7,
title = "In vivo validation of a miniaturised electrochemical oxygen sensor for measuring intestinal oxygen tension",
abstract = "Recent advances in the fields of electronics and microfabrication techniques have led to the development of implantable medical devices for use within the field of precision medicine. Monitoring visceral surface tissue O2 tension (ptO2) by means of an implantable sensor is potentially useful in many clinical situations including the peri-operative management of patients undergoing intestinal resection and anastomosis. This concept could provide a means by which treatment could be tailored to individual patients. This study describes the in vivo validation of a novel miniaturised electrochemical O2 sensor to provide real-time data on intestinal ptO2. A single O2 sensor was placed onto the serosal surface of the small intestine of anaesthetised rats that were exposed to ischaemic (superior mesenteric artery occlusion) and hypoxaemic (alterations in inspired fractional O2 concentrations) insults. Control experiments demonstrated that the sensors function and remain stable in an in vivo environment. Intestinal ptO2 decreased following superior mesenteric artery occlusion and with reductions in inspired O2 concentrations. These results were reversible after reinstating blood flow or increasing inspired O2 concentrations. We have successfully developed an anaesthetised rat intestinal ischaemic and hypoxic model for validation of a miniaturised O2 sensor to provide real-time measurement of intestinal ptO2. Our results support further validation of the sensors in physiological conditions using a large animal model to provide evidence of their use in clinical applications where monitoring visceral surface tissue O2 tension is important.",
keywords = "hypoxaemia, intestinal resection and anastomosis, intestinal serosal tissue oxygen tension, miniaturised electrochemical oxygen sensor, superior mesenteric artery occlusion",
author = "Gray, {Mark E.} and Marland, {Jamie R. K.} and Camelia Dunare and Blair, {Ewen O.} and James Meehan and Andreas Tsiamis and Kunkler, {Ian H.} and Murray, {Alan F.} and David Argyle and Alex Dyson and Mervyn Singer and Potter, {Mark A.}",
year = "2019",
month = "8",
day = "1",
doi = "10.1152/ajpgi.00050.2019",
language = "English",
volume = "317",
pages = "G242--G252",
journal = "American Journal of Physiology-Gastrointestinal and Liver Physiology",
issn = "0193-1857",
number = "2",

}

Gray, ME, Marland, JRK, Dunare, C, Blair, EO, Meehan, J, Tsiamis, A, Kunkler, IH, Murray, AF, Argyle, D, Dyson, A, Singer, M & Potter, MA 2019, 'In vivo validation of a miniaturised electrochemical oxygen sensor for measuring intestinal oxygen tension' American Journal of Physiology-Gastrointestinal and Liver Physiology, vol. 317, no. 2, pp. G242-G252. https://doi.org/10.1152/ajpgi.00050.2019

In vivo validation of a miniaturised electrochemical oxygen sensor for measuring intestinal oxygen tension. / Gray, Mark E.; Marland, Jamie R. K.; Dunare, Camelia; Blair, Ewen O.; Meehan, James; Tsiamis, Andreas; Kunkler, Ian H.; Murray, Alan F.; Argyle, David; Dyson, Alex; Singer, Mervyn; Potter, Mark A.

In: American Journal of Physiology-Gastrointestinal and Liver Physiology, Vol. 317, No. 2, 01.08.2019, p. G242-G252.

Research output: Contribution to journalArticle

TY - JOUR

T1 - In vivo validation of a miniaturised electrochemical oxygen sensor for measuring intestinal oxygen tension

AU - Gray, Mark E.

AU - Marland, Jamie R. K.

AU - Dunare, Camelia

AU - Blair, Ewen O.

AU - Meehan, James

AU - Tsiamis, Andreas

AU - Kunkler, Ian H.

AU - Murray, Alan F.

AU - Argyle, David

AU - Dyson, Alex

AU - Singer, Mervyn

AU - Potter, Mark A.

PY - 2019/8/1

Y1 - 2019/8/1

N2 - Recent advances in the fields of electronics and microfabrication techniques have led to the development of implantable medical devices for use within the field of precision medicine. Monitoring visceral surface tissue O2 tension (ptO2) by means of an implantable sensor is potentially useful in many clinical situations including the peri-operative management of patients undergoing intestinal resection and anastomosis. This concept could provide a means by which treatment could be tailored to individual patients. This study describes the in vivo validation of a novel miniaturised electrochemical O2 sensor to provide real-time data on intestinal ptO2. A single O2 sensor was placed onto the serosal surface of the small intestine of anaesthetised rats that were exposed to ischaemic (superior mesenteric artery occlusion) and hypoxaemic (alterations in inspired fractional O2 concentrations) insults. Control experiments demonstrated that the sensors function and remain stable in an in vivo environment. Intestinal ptO2 decreased following superior mesenteric artery occlusion and with reductions in inspired O2 concentrations. These results were reversible after reinstating blood flow or increasing inspired O2 concentrations. We have successfully developed an anaesthetised rat intestinal ischaemic and hypoxic model for validation of a miniaturised O2 sensor to provide real-time measurement of intestinal ptO2. Our results support further validation of the sensors in physiological conditions using a large animal model to provide evidence of their use in clinical applications where monitoring visceral surface tissue O2 tension is important.

AB - Recent advances in the fields of electronics and microfabrication techniques have led to the development of implantable medical devices for use within the field of precision medicine. Monitoring visceral surface tissue O2 tension (ptO2) by means of an implantable sensor is potentially useful in many clinical situations including the peri-operative management of patients undergoing intestinal resection and anastomosis. This concept could provide a means by which treatment could be tailored to individual patients. This study describes the in vivo validation of a novel miniaturised electrochemical O2 sensor to provide real-time data on intestinal ptO2. A single O2 sensor was placed onto the serosal surface of the small intestine of anaesthetised rats that were exposed to ischaemic (superior mesenteric artery occlusion) and hypoxaemic (alterations in inspired fractional O2 concentrations) insults. Control experiments demonstrated that the sensors function and remain stable in an in vivo environment. Intestinal ptO2 decreased following superior mesenteric artery occlusion and with reductions in inspired O2 concentrations. These results were reversible after reinstating blood flow or increasing inspired O2 concentrations. We have successfully developed an anaesthetised rat intestinal ischaemic and hypoxic model for validation of a miniaturised O2 sensor to provide real-time measurement of intestinal ptO2. Our results support further validation of the sensors in physiological conditions using a large animal model to provide evidence of their use in clinical applications where monitoring visceral surface tissue O2 tension is important.

KW - hypoxaemia

KW - intestinal resection and anastomosis

KW - intestinal serosal tissue oxygen tension

KW - miniaturised electrochemical oxygen sensor

KW - superior mesenteric artery occlusion

U2 - 10.1152/ajpgi.00050.2019

DO - 10.1152/ajpgi.00050.2019

M3 - Article

VL - 317

SP - G242-G252

JO - American Journal of Physiology-Gastrointestinal and Liver Physiology

T2 - American Journal of Physiology-Gastrointestinal and Liver Physiology

JF - American Journal of Physiology-Gastrointestinal and Liver Physiology

SN - 0193-1857

IS - 2

ER -