Projects per year
Abstract
Vascular immune-inflammatory responses play a crucial role in the progression and outcome of atherosclerosis. The ability to assess localized inflammation through detection of specific vascular inflammatory biomarkers would significantly improve cardiovascular risk assessment and management; however, no multi parameter molecular imaging technologies have been established to date. Here, we report the targeted in vivo imaging of multiple vascular biomarkers using antibody-functionalized nanoparticles and surface-enhanced Raman scattering (SERS).
Methods: A series of antibody-functionalized gold nanoprobes (BFNP) were designed containing unique Raman signals in order to detect intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1) and P-selectin using SERS.
Results: SERS and BFNP were utilized to detect, discriminate and quantify ICAM-1, VCAM-1 and P-selectin in vitro on human endothelial cells and ex vivo in human coronary arteries. Ultimately, non-invasive multiplex imaging of adhesion molecules in a humanized mouse model was demonstrated in vivo following intravenous injection of the nanoprobes.
Conclusion: This study demonstrates that multiplexed SERS-based molecular imaging can indicate the status of vascular inflammation in vivo and gives promise for SERS as a clinical imaging technique for cardiovascular disease in the future.
Methods: A series of antibody-functionalized gold nanoprobes (BFNP) were designed containing unique Raman signals in order to detect intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1) and P-selectin using SERS.
Results: SERS and BFNP were utilized to detect, discriminate and quantify ICAM-1, VCAM-1 and P-selectin in vitro on human endothelial cells and ex vivo in human coronary arteries. Ultimately, non-invasive multiplex imaging of adhesion molecules in a humanized mouse model was demonstrated in vivo following intravenous injection of the nanoprobes.
Conclusion: This study demonstrates that multiplexed SERS-based molecular imaging can indicate the status of vascular inflammation in vivo and gives promise for SERS as a clinical imaging technique for cardiovascular disease in the future.
Original language | English |
---|---|
Pages (from-to) | 6195-6209 |
Number of pages | 15 |
Journal | Theranostics |
Volume | 8 |
Issue number | 22 |
Early online date | 29 Nov 2018 |
DOIs | |
Publication status | E-pub ahead of print - 29 Nov 2018 |
Keywords
- atherosclerosis
- molecular imaging
- multiplexing
- vascular inflammation
- surface-enhanced Raman spectroscopy (SERS)
Fingerprint
Dive into the research topics of 'In vivo multiplex molecular imaging of vascular inflammation using surface-enhanced Raman spectroscopy'. Together they form a unique fingerprint.Profiles
Projects
- 1 Finished
-
In Situ Nanoparticle Assemblies for Healthcare Diagnostics and Therapy
Graham, D. (Principal Investigator) & Faulds, K. (Co-investigator)
EPSRC (Engineering and Physical Sciences Research Council)
1/05/14 → 31/10/18
Project: Research